Evaluation of cell toxicity and DNA and protein binding of green synthesized silver nanoparticles
Autor(a) principal: | |
---|---|
Data de Publicação: | 2018 |
Outros Autores: | , , , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
Texto Completo: | http://hdl.handle.net/10400.21/9121 |
Resumo: | Silver nanoparticles (AgNPs) were prepared by GREEN chemistry relying on the reduction of AgNO3 by phytochemicals present in black tea extract. AgNPs were fully characterized by transmission electron microscopy (TEM), ultraviolet-visible spectroscopy ((UV-vis)), X-ray diffraction (XRD) and energy dispersive absorption spectroscopy (EDS). The synthesized AgNPs induced a decrease of the cell viability in a dose-dependent manner with a low IC50 (0.5 +/- 0.1 mu M) for an ovarian carcinoma cell line (A2780) compared to primary human fibroblasts (IC50 5.0 +/- 0.1 mu M). The DNA binding capability of CT (calf thymus) DNA was investigated using electronic absorption and fluorescence spectroscopies, circular dichroism and viscosity titration methods. Additionally, the AgNPs strongly quench the intrinsic fluorescence of BSA, as determined by synchronous fluorescence spectra. |
id |
RCAP_10bd1e2d8f11de0698c7b06f8f240834 |
---|---|
oai_identifier_str |
oai:repositorio.ipl.pt:10400.21/9121 |
network_acronym_str |
RCAP |
network_name_str |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
repository_id_str |
7160 |
spelling |
Evaluation of cell toxicity and DNA and protein binding of green synthesized silver nanoparticlesGreen chemistrySilver nanoparticlesInteractionCytotoxicityCancer cell linesSilver nanoparticles (AgNPs) were prepared by GREEN chemistry relying on the reduction of AgNO3 by phytochemicals present in black tea extract. AgNPs were fully characterized by transmission electron microscopy (TEM), ultraviolet-visible spectroscopy ((UV-vis)), X-ray diffraction (XRD) and energy dispersive absorption spectroscopy (EDS). The synthesized AgNPs induced a decrease of the cell viability in a dose-dependent manner with a low IC50 (0.5 +/- 0.1 mu M) for an ovarian carcinoma cell line (A2780) compared to primary human fibroblasts (IC50 5.0 +/- 0.1 mu M). The DNA binding capability of CT (calf thymus) DNA was investigated using electronic absorption and fluorescence spectroscopies, circular dichroism and viscosity titration methods. Additionally, the AgNPs strongly quench the intrinsic fluorescence of BSA, as determined by synchronous fluorescence spectra.ElsevierRCIPLDa Costa Ribeiro, Ana PaulaAnbu, SAlegria, ElisabeteFernandes, AlexandraBaptista, PedroMendes, RitaMatias, A. S.Mendes, M.Guedes Da Silva, M. Fátima C.Pombeiro, Armando2018-12-03T12:02:02Z2018-052018-05-01T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/10400.21/9121engRIBEIRO, A. P. C.; [et al] – Evaluation of cell toxicity and DNA and protein binding of green synthesized silver nanoparticles. Biomedicine & Pharmacotherapy. ISSN 0753-3322. Vol. 101, (2018), pp. 137-1440753-332210.1016/j.biopha.2018.02.069info:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2023-08-03T09:57:26Zoai:repositorio.ipl.pt:10400.21/9121Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-19T20:17:45.739671Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse |
dc.title.none.fl_str_mv |
Evaluation of cell toxicity and DNA and protein binding of green synthesized silver nanoparticles |
title |
Evaluation of cell toxicity and DNA and protein binding of green synthesized silver nanoparticles |
spellingShingle |
Evaluation of cell toxicity and DNA and protein binding of green synthesized silver nanoparticles Da Costa Ribeiro, Ana Paula Green chemistry Silver nanoparticles Interaction Cytotoxicity Cancer cell lines |
title_short |
Evaluation of cell toxicity and DNA and protein binding of green synthesized silver nanoparticles |
title_full |
Evaluation of cell toxicity and DNA and protein binding of green synthesized silver nanoparticles |
title_fullStr |
Evaluation of cell toxicity and DNA and protein binding of green synthesized silver nanoparticles |
title_full_unstemmed |
Evaluation of cell toxicity and DNA and protein binding of green synthesized silver nanoparticles |
title_sort |
Evaluation of cell toxicity and DNA and protein binding of green synthesized silver nanoparticles |
author |
Da Costa Ribeiro, Ana Paula |
author_facet |
Da Costa Ribeiro, Ana Paula Anbu, S Alegria, Elisabete Fernandes, Alexandra Baptista, Pedro Mendes, Rita Matias, A. S. Mendes, M. Guedes Da Silva, M. Fátima C. Pombeiro, Armando |
author_role |
author |
author2 |
Anbu, S Alegria, Elisabete Fernandes, Alexandra Baptista, Pedro Mendes, Rita Matias, A. S. Mendes, M. Guedes Da Silva, M. Fátima C. Pombeiro, Armando |
author2_role |
author author author author author author author author author |
dc.contributor.none.fl_str_mv |
RCIPL |
dc.contributor.author.fl_str_mv |
Da Costa Ribeiro, Ana Paula Anbu, S Alegria, Elisabete Fernandes, Alexandra Baptista, Pedro Mendes, Rita Matias, A. S. Mendes, M. Guedes Da Silva, M. Fátima C. Pombeiro, Armando |
dc.subject.por.fl_str_mv |
Green chemistry Silver nanoparticles Interaction Cytotoxicity Cancer cell lines |
topic |
Green chemistry Silver nanoparticles Interaction Cytotoxicity Cancer cell lines |
description |
Silver nanoparticles (AgNPs) were prepared by GREEN chemistry relying on the reduction of AgNO3 by phytochemicals present in black tea extract. AgNPs were fully characterized by transmission electron microscopy (TEM), ultraviolet-visible spectroscopy ((UV-vis)), X-ray diffraction (XRD) and energy dispersive absorption spectroscopy (EDS). The synthesized AgNPs induced a decrease of the cell viability in a dose-dependent manner with a low IC50 (0.5 +/- 0.1 mu M) for an ovarian carcinoma cell line (A2780) compared to primary human fibroblasts (IC50 5.0 +/- 0.1 mu M). The DNA binding capability of CT (calf thymus) DNA was investigated using electronic absorption and fluorescence spectroscopies, circular dichroism and viscosity titration methods. Additionally, the AgNPs strongly quench the intrinsic fluorescence of BSA, as determined by synchronous fluorescence spectra. |
publishDate |
2018 |
dc.date.none.fl_str_mv |
2018-12-03T12:02:02Z 2018-05 2018-05-01T00:00:00Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://hdl.handle.net/10400.21/9121 |
url |
http://hdl.handle.net/10400.21/9121 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
RIBEIRO, A. P. C.; [et al] – Evaluation of cell toxicity and DNA and protein binding of green synthesized silver nanoparticles. Biomedicine & Pharmacotherapy. ISSN 0753-3322. Vol. 101, (2018), pp. 137-144 0753-3322 10.1016/j.biopha.2018.02.069 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.publisher.none.fl_str_mv |
Elsevier |
publisher.none.fl_str_mv |
Elsevier |
dc.source.none.fl_str_mv |
reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação instacron:RCAAP |
instname_str |
Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
instacron_str |
RCAAP |
institution |
RCAAP |
reponame_str |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
collection |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
repository.name.fl_str_mv |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
repository.mail.fl_str_mv |
|
_version_ |
1799133440870711296 |