Evidence of sharing of Klebsiella pneumoniae strains between healthy companion animals and cohabiting humans

Detalhes bibliográficos
Autor(a) principal: Marques, Cátia Filipa Saraiva
Data de Publicação: 2019
Outros Autores: Belas, Adriana, Aboim, Catarina, Cavaco-Silva, Patrícia, Trigueiro, Graça, Gama, Luis, Pomba, C.
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
Texto Completo: http://hdl.handle.net/10400.5/22110
Resumo: Research Areas: Microbiology
id RCAP_10c42e14b873eb0ac8b81c6bd3a41d9e
oai_identifier_str oai:www.repository.utl.pt:10400.5/22110
network_acronym_str RCAP
network_name_str Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
repository_id_str 7160
spelling Evidence of sharing of Klebsiella pneumoniae strains between healthy companion animals and cohabiting humansKlebsiella pneumoniaeanimal-human sharingclonal relatednesscompanion animalsdoghumansResearch Areas: MicrobiologyThis study aimed to characterize the fecal colonization and sharing of Klebsiella pneumoniae strains between companion animals and humans living in close contact. Fecal samples were collected from 50 healthy participants (24 humans, 18 dogs, and 8 cats) belonging to 18 households. Samples were plated onto MacConkey agar (MCK) plates with and without cefotaxime or meropenem supplementation. Up to five K. pneumoniae colonies per participant were compared by pulsed-field gel electrophoresis (PFGE) after XbaI restriction. K. pneumoniae strains with unique pulse types from each participant were characterized for antimicrobial susceptibility, virulence genes, and multilocus sequence type (MLST). Fecal K. pneumoniae pulse types were compared to those of clinical K. pneumoniae strains from animal and human patients with urinary tract infections (n = 104). K. pneumoniae colonization was detected in nonsupplemented MCK in around 38% of dogs (n = 7) and humans (n = 9). K. pneumoniae strains isolated from dogs belonged to sequence type 17 (ST17), ST188, ST252, ST281, ST423, ST1093, ST1241, ST3398, and ST3399. None of the K. pneumoniae strains were multidrug resistant or hypervirulent. Two households included multiple colonized participants. Notably, two colonized dogs within household 15 (H15) shared a strain each (ST252 and ST1241) with one coliving human. One dog from H16 shared one PFGE-undistinguishable K. pneumoniae ST17 strain with two humans from different households; however, the antimicrobial susceptibility phenotypes of these three strains differed. Two main virulence genotypes were detected, namely fimH-1 mrkD ycfM entB kfu and fimH-1 mrkD ycfM entB kpn. These results highlight the potential role of dogs as a reservoir of K. pneumoniae to humans and vice versa. Furthermore, to our best knowledge, this is the first report of healthy humans and dogs sharing K. pneumoniae strains that were undistinguishable by PFGE/MLST.American Society for MicrobiologyRepositório da Universidade de LisboaMarques, Cátia Filipa SaraivaBelas, AdrianaAboim, CatarinaCavaco-Silva, PatríciaTrigueiro, GraçaGama, LuisPomba, C.2021-09-29T23:16:22Z2019-04-032019-04-03T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/10400.5/22110engMarques C, Belas A, Aboim C, Cavaco-Silva P, Trigueiro G, Gama LT, Pomba C. 2019. Evidence of sharing of Klebsiella pneumoniae strains between healthy companion animals and cohabiting humans [article]. Journal of Clinical Microbiology, 57:6, e01537-18. Doi.org/10.1128/JCM.01537-18.10.1128/JCM.01537-181098-660Xinfo:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2023-03-06T14:51:40Zoai:www.repository.utl.pt:10400.5/22110Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-19T17:06:36.524478Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse
dc.title.none.fl_str_mv Evidence of sharing of Klebsiella pneumoniae strains between healthy companion animals and cohabiting humans
title Evidence of sharing of Klebsiella pneumoniae strains between healthy companion animals and cohabiting humans
spellingShingle Evidence of sharing of Klebsiella pneumoniae strains between healthy companion animals and cohabiting humans
Marques, Cátia Filipa Saraiva
Klebsiella pneumoniae
animal-human sharing
clonal relatedness
companion animals
dog
humans
title_short Evidence of sharing of Klebsiella pneumoniae strains between healthy companion animals and cohabiting humans
title_full Evidence of sharing of Klebsiella pneumoniae strains between healthy companion animals and cohabiting humans
title_fullStr Evidence of sharing of Klebsiella pneumoniae strains between healthy companion animals and cohabiting humans
title_full_unstemmed Evidence of sharing of Klebsiella pneumoniae strains between healthy companion animals and cohabiting humans
title_sort Evidence of sharing of Klebsiella pneumoniae strains between healthy companion animals and cohabiting humans
author Marques, Cátia Filipa Saraiva
author_facet Marques, Cátia Filipa Saraiva
Belas, Adriana
Aboim, Catarina
Cavaco-Silva, Patrícia
Trigueiro, Graça
Gama, Luis
Pomba, C.
author_role author
author2 Belas, Adriana
Aboim, Catarina
Cavaco-Silva, Patrícia
Trigueiro, Graça
Gama, Luis
Pomba, C.
author2_role author
author
author
author
author
author
dc.contributor.none.fl_str_mv Repositório da Universidade de Lisboa
dc.contributor.author.fl_str_mv Marques, Cátia Filipa Saraiva
Belas, Adriana
Aboim, Catarina
Cavaco-Silva, Patrícia
Trigueiro, Graça
Gama, Luis
Pomba, C.
dc.subject.por.fl_str_mv Klebsiella pneumoniae
animal-human sharing
clonal relatedness
companion animals
dog
humans
topic Klebsiella pneumoniae
animal-human sharing
clonal relatedness
companion animals
dog
humans
description Research Areas: Microbiology
publishDate 2019
dc.date.none.fl_str_mv 2019-04-03
2019-04-03T00:00:00Z
2021-09-29T23:16:22Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://hdl.handle.net/10400.5/22110
url http://hdl.handle.net/10400.5/22110
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv Marques C, Belas A, Aboim C, Cavaco-Silva P, Trigueiro G, Gama LT, Pomba C. 2019. Evidence of sharing of Klebsiella pneumoniae strains between healthy companion animals and cohabiting humans [article]. Journal of Clinical Microbiology, 57:6, e01537-18. Doi.org/10.1128/JCM.01537-18.
10.1128/JCM.01537-18
1098-660X
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv American Society for Microbiology
publisher.none.fl_str_mv American Society for Microbiology
dc.source.none.fl_str_mv reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron:RCAAP
instname_str Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron_str RCAAP
institution RCAAP
reponame_str Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
collection Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
repository.name.fl_str_mv Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
repository.mail.fl_str_mv
_version_ 1799131158657630208

Registros relacionados