Optimization of the determination of tocopherols in Agaricus sp. edible mushrooms by a normal phase liquid chromatographic method
Autor(a) principal: | |
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Data de Publicação: | 2008 |
Outros Autores: | , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
Texto Completo: | http://hdl.handle.net/10198/838 |
Resumo: | Individual tocopherol profile of five Agaricus mushroom species, widely consumed in Portugal, was obtained by a normal-phase high-performance liquid chromatography (NP-HPLC). It was used a simple solid–liquid extraction procedure without saponification step and the chromatographic separation was achieved using a YMC-Pack Polyamine II column using an isocratic elution with hexane/ethyl acetate (70:30, v/v) at a flow rate of 1.0 mL/min. The effluent was monitored by a fluorescence detector. All the compounds were separated in a period of time of 30 min. The method proved to be sensitive, reproducible and accurate, allowing the determination of tocopherols. |
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Optimization of the determination of tocopherols in Agaricus sp. edible mushrooms by a normal phase liquid chromatographic methodVitamin EHPLC AnalysisMushroomsIndividual tocopherol profile of five Agaricus mushroom species, widely consumed in Portugal, was obtained by a normal-phase high-performance liquid chromatography (NP-HPLC). It was used a simple solid–liquid extraction procedure without saponification step and the chromatographic separation was achieved using a YMC-Pack Polyamine II column using an isocratic elution with hexane/ethyl acetate (70:30, v/v) at a flow rate of 1.0 mL/min. The effluent was monitored by a fluorescence detector. All the compounds were separated in a period of time of 30 min. The method proved to be sensitive, reproducible and accurate, allowing the determination of tocopherols.ElsevierBiblioteca Digital do IPBBarros, LillianCorreia, Daniela M.Ferreira, Isabel C.F.R.Baptista, PaulaSantos-Buelga, Celestino2008-09-12T16:03:18Z20082008-01-01T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/10198/838engFood Chemistry. ISSN 0308-8146. 110:4 (2008) p. 1046-10500308-814610.1016/j.foodchem.2008.03.016info:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2023-11-21T10:03:57Zoai:bibliotecadigital.ipb.pt:10198/838Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-19T22:54:28.543086Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse |
dc.title.none.fl_str_mv |
Optimization of the determination of tocopherols in Agaricus sp. edible mushrooms by a normal phase liquid chromatographic method |
title |
Optimization of the determination of tocopherols in Agaricus sp. edible mushrooms by a normal phase liquid chromatographic method |
spellingShingle |
Optimization of the determination of tocopherols in Agaricus sp. edible mushrooms by a normal phase liquid chromatographic method Barros, Lillian Vitamin E HPLC Analysis Mushrooms |
title_short |
Optimization of the determination of tocopherols in Agaricus sp. edible mushrooms by a normal phase liquid chromatographic method |
title_full |
Optimization of the determination of tocopherols in Agaricus sp. edible mushrooms by a normal phase liquid chromatographic method |
title_fullStr |
Optimization of the determination of tocopherols in Agaricus sp. edible mushrooms by a normal phase liquid chromatographic method |
title_full_unstemmed |
Optimization of the determination of tocopherols in Agaricus sp. edible mushrooms by a normal phase liquid chromatographic method |
title_sort |
Optimization of the determination of tocopherols in Agaricus sp. edible mushrooms by a normal phase liquid chromatographic method |
author |
Barros, Lillian |
author_facet |
Barros, Lillian Correia, Daniela M. Ferreira, Isabel C.F.R. Baptista, Paula Santos-Buelga, Celestino |
author_role |
author |
author2 |
Correia, Daniela M. Ferreira, Isabel C.F.R. Baptista, Paula Santos-Buelga, Celestino |
author2_role |
author author author author |
dc.contributor.none.fl_str_mv |
Biblioteca Digital do IPB |
dc.contributor.author.fl_str_mv |
Barros, Lillian Correia, Daniela M. Ferreira, Isabel C.F.R. Baptista, Paula Santos-Buelga, Celestino |
dc.subject.por.fl_str_mv |
Vitamin E HPLC Analysis Mushrooms |
topic |
Vitamin E HPLC Analysis Mushrooms |
description |
Individual tocopherol profile of five Agaricus mushroom species, widely consumed in Portugal, was obtained by a normal-phase high-performance liquid chromatography (NP-HPLC). It was used a simple solid–liquid extraction procedure without saponification step and the chromatographic separation was achieved using a YMC-Pack Polyamine II column using an isocratic elution with hexane/ethyl acetate (70:30, v/v) at a flow rate of 1.0 mL/min. The effluent was monitored by a fluorescence detector. All the compounds were separated in a period of time of 30 min. The method proved to be sensitive, reproducible and accurate, allowing the determination of tocopherols. |
publishDate |
2008 |
dc.date.none.fl_str_mv |
2008-09-12T16:03:18Z 2008 2008-01-01T00:00:00Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://hdl.handle.net/10198/838 |
url |
http://hdl.handle.net/10198/838 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Food Chemistry. ISSN 0308-8146. 110:4 (2008) p. 1046-1050 0308-8146 10.1016/j.foodchem.2008.03.016 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.publisher.none.fl_str_mv |
Elsevier |
publisher.none.fl_str_mv |
Elsevier |
dc.source.none.fl_str_mv |
reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação instacron:RCAAP |
instname_str |
Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
instacron_str |
RCAAP |
institution |
RCAAP |
reponame_str |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
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Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
repository.name.fl_str_mv |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
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