Understanding the physiology of glutamate receptors by use of a protocol for neuronal staining

Detalhes bibliográficos
Autor(a) principal: Malva, João O.
Data de Publicação: 2003
Outros Autores: Vieira, Ana P., Oliveira, Catarina R.
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
Texto Completo: http://hdl.handle.net/10316/12639
https://doi.org/10.1152/advan.00053.2002
Resumo: Teaching students about the physiology of neurotransmitter receptors usually requires practical lessons with the use of sophisticated equipment and complex analysis of data. Here, we report our experience in teaching medical students with a simple, practical protocol that transforms the physiology of glutamate receptors into neuronal staining, observable under bright-field microscopy. Essentially, the students were challenged to selectively stain a subpopulation of cultured neurons expressing Ca(2+)-permeable alpha-amino-3-hydroxy-5-methylisoxazole-4-propionate (AMPA) receptors (a subgroup of ionotropic glutamate receptors). Neurons expressing this type of receptors were loaded with Co(2+) (in substitution for Ca(2+)) after nondesensitizing activation of AMPA receptors. After precipitation, the Co(2+) was revealed after treatment with silver. At the end of the procedure, the neurons expressing Ca(2+)-permeable AMPA receptors were visually identified under bright-field microscopy. The procedure allowed the visualization of the complete dendritic network of the stained neurons and allowed the students to learn very efficiently about the physiology of glutamate receptors
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spelling Understanding the physiology of glutamate receptors by use of a protocol for neuronal stainingNeuronsCobalt StainingTeaching students about the physiology of neurotransmitter receptors usually requires practical lessons with the use of sophisticated equipment and complex analysis of data. Here, we report our experience in teaching medical students with a simple, practical protocol that transforms the physiology of glutamate receptors into neuronal staining, observable under bright-field microscopy. Essentially, the students were challenged to selectively stain a subpopulation of cultured neurons expressing Ca(2+)-permeable alpha-amino-3-hydroxy-5-methylisoxazole-4-propionate (AMPA) receptors (a subgroup of ionotropic glutamate receptors). Neurons expressing this type of receptors were loaded with Co(2+) (in substitution for Ca(2+)) after nondesensitizing activation of AMPA receptors. After precipitation, the Co(2+) was revealed after treatment with silver. At the end of the procedure, the neurons expressing Ca(2+)-permeable AMPA receptors were visually identified under bright-field microscopy. The procedure allowed the visualization of the complete dendritic network of the stained neurons and allowed the students to learn very efficiently about the physiology of glutamate receptorsThe American Physiological Society2003-12info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articlehttp://hdl.handle.net/10316/12639http://hdl.handle.net/10316/12639https://doi.org/10.1152/advan.00053.2002engAdvances in Physiology Education. 27:1-4 (2003) 78-851043-4046Malva, João O.Vieira, Ana P.Oliveira, Catarina R.info:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2022-05-25T04:06:32Zoai:estudogeral.uc.pt:10316/12639Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-19T20:43:37.574957Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse
dc.title.none.fl_str_mv Understanding the physiology of glutamate receptors by use of a protocol for neuronal staining
title Understanding the physiology of glutamate receptors by use of a protocol for neuronal staining
spellingShingle Understanding the physiology of glutamate receptors by use of a protocol for neuronal staining
Malva, João O.
Neurons
Cobalt Staining
title_short Understanding the physiology of glutamate receptors by use of a protocol for neuronal staining
title_full Understanding the physiology of glutamate receptors by use of a protocol for neuronal staining
title_fullStr Understanding the physiology of glutamate receptors by use of a protocol for neuronal staining
title_full_unstemmed Understanding the physiology of glutamate receptors by use of a protocol for neuronal staining
title_sort Understanding the physiology of glutamate receptors by use of a protocol for neuronal staining
author Malva, João O.
author_facet Malva, João O.
Vieira, Ana P.
Oliveira, Catarina R.
author_role author
author2 Vieira, Ana P.
Oliveira, Catarina R.
author2_role author
author
dc.contributor.author.fl_str_mv Malva, João O.
Vieira, Ana P.
Oliveira, Catarina R.
dc.subject.por.fl_str_mv Neurons
Cobalt Staining
topic Neurons
Cobalt Staining
description Teaching students about the physiology of neurotransmitter receptors usually requires practical lessons with the use of sophisticated equipment and complex analysis of data. Here, we report our experience in teaching medical students with a simple, practical protocol that transforms the physiology of glutamate receptors into neuronal staining, observable under bright-field microscopy. Essentially, the students were challenged to selectively stain a subpopulation of cultured neurons expressing Ca(2+)-permeable alpha-amino-3-hydroxy-5-methylisoxazole-4-propionate (AMPA) receptors (a subgroup of ionotropic glutamate receptors). Neurons expressing this type of receptors were loaded with Co(2+) (in substitution for Ca(2+)) after nondesensitizing activation of AMPA receptors. After precipitation, the Co(2+) was revealed after treatment with silver. At the end of the procedure, the neurons expressing Ca(2+)-permeable AMPA receptors were visually identified under bright-field microscopy. The procedure allowed the visualization of the complete dendritic network of the stained neurons and allowed the students to learn very efficiently about the physiology of glutamate receptors
publishDate 2003
dc.date.none.fl_str_mv 2003-12
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://hdl.handle.net/10316/12639
http://hdl.handle.net/10316/12639
https://doi.org/10.1152/advan.00053.2002
url http://hdl.handle.net/10316/12639
https://doi.org/10.1152/advan.00053.2002
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv Advances in Physiology Education. 27:1-4 (2003) 78-85
1043-4046
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
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dc.publisher.none.fl_str_mv The American Physiological Society
publisher.none.fl_str_mv The American Physiological Society
dc.source.none.fl_str_mv reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
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