Understanding the physiology of glutamate receptors by use of a protocol for neuronal staining
Autor(a) principal: | |
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Data de Publicação: | 2003 |
Outros Autores: | , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
Texto Completo: | http://hdl.handle.net/10316/12639 https://doi.org/10.1152/advan.00053.2002 |
Resumo: | Teaching students about the physiology of neurotransmitter receptors usually requires practical lessons with the use of sophisticated equipment and complex analysis of data. Here, we report our experience in teaching medical students with a simple, practical protocol that transforms the physiology of glutamate receptors into neuronal staining, observable under bright-field microscopy. Essentially, the students were challenged to selectively stain a subpopulation of cultured neurons expressing Ca(2+)-permeable alpha-amino-3-hydroxy-5-methylisoxazole-4-propionate (AMPA) receptors (a subgroup of ionotropic glutamate receptors). Neurons expressing this type of receptors were loaded with Co(2+) (in substitution for Ca(2+)) after nondesensitizing activation of AMPA receptors. After precipitation, the Co(2+) was revealed after treatment with silver. At the end of the procedure, the neurons expressing Ca(2+)-permeable AMPA receptors were visually identified under bright-field microscopy. The procedure allowed the visualization of the complete dendritic network of the stained neurons and allowed the students to learn very efficiently about the physiology of glutamate receptors |
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Understanding the physiology of glutamate receptors by use of a protocol for neuronal stainingNeuronsCobalt StainingTeaching students about the physiology of neurotransmitter receptors usually requires practical lessons with the use of sophisticated equipment and complex analysis of data. Here, we report our experience in teaching medical students with a simple, practical protocol that transforms the physiology of glutamate receptors into neuronal staining, observable under bright-field microscopy. Essentially, the students were challenged to selectively stain a subpopulation of cultured neurons expressing Ca(2+)-permeable alpha-amino-3-hydroxy-5-methylisoxazole-4-propionate (AMPA) receptors (a subgroup of ionotropic glutamate receptors). Neurons expressing this type of receptors were loaded with Co(2+) (in substitution for Ca(2+)) after nondesensitizing activation of AMPA receptors. After precipitation, the Co(2+) was revealed after treatment with silver. At the end of the procedure, the neurons expressing Ca(2+)-permeable AMPA receptors were visually identified under bright-field microscopy. The procedure allowed the visualization of the complete dendritic network of the stained neurons and allowed the students to learn very efficiently about the physiology of glutamate receptorsThe American Physiological Society2003-12info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articlehttp://hdl.handle.net/10316/12639http://hdl.handle.net/10316/12639https://doi.org/10.1152/advan.00053.2002engAdvances in Physiology Education. 27:1-4 (2003) 78-851043-4046Malva, João O.Vieira, Ana P.Oliveira, Catarina R.info:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2022-05-25T04:06:32Zoai:estudogeral.uc.pt:10316/12639Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-19T20:43:37.574957Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse |
dc.title.none.fl_str_mv |
Understanding the physiology of glutamate receptors by use of a protocol for neuronal staining |
title |
Understanding the physiology of glutamate receptors by use of a protocol for neuronal staining |
spellingShingle |
Understanding the physiology of glutamate receptors by use of a protocol for neuronal staining Malva, João O. Neurons Cobalt Staining |
title_short |
Understanding the physiology of glutamate receptors by use of a protocol for neuronal staining |
title_full |
Understanding the physiology of glutamate receptors by use of a protocol for neuronal staining |
title_fullStr |
Understanding the physiology of glutamate receptors by use of a protocol for neuronal staining |
title_full_unstemmed |
Understanding the physiology of glutamate receptors by use of a protocol for neuronal staining |
title_sort |
Understanding the physiology of glutamate receptors by use of a protocol for neuronal staining |
author |
Malva, João O. |
author_facet |
Malva, João O. Vieira, Ana P. Oliveira, Catarina R. |
author_role |
author |
author2 |
Vieira, Ana P. Oliveira, Catarina R. |
author2_role |
author author |
dc.contributor.author.fl_str_mv |
Malva, João O. Vieira, Ana P. Oliveira, Catarina R. |
dc.subject.por.fl_str_mv |
Neurons Cobalt Staining |
topic |
Neurons Cobalt Staining |
description |
Teaching students about the physiology of neurotransmitter receptors usually requires practical lessons with the use of sophisticated equipment and complex analysis of data. Here, we report our experience in teaching medical students with a simple, practical protocol that transforms the physiology of glutamate receptors into neuronal staining, observable under bright-field microscopy. Essentially, the students were challenged to selectively stain a subpopulation of cultured neurons expressing Ca(2+)-permeable alpha-amino-3-hydroxy-5-methylisoxazole-4-propionate (AMPA) receptors (a subgroup of ionotropic glutamate receptors). Neurons expressing this type of receptors were loaded with Co(2+) (in substitution for Ca(2+)) after nondesensitizing activation of AMPA receptors. After precipitation, the Co(2+) was revealed after treatment with silver. At the end of the procedure, the neurons expressing Ca(2+)-permeable AMPA receptors were visually identified under bright-field microscopy. The procedure allowed the visualization of the complete dendritic network of the stained neurons and allowed the students to learn very efficiently about the physiology of glutamate receptors |
publishDate |
2003 |
dc.date.none.fl_str_mv |
2003-12 |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://hdl.handle.net/10316/12639 http://hdl.handle.net/10316/12639 https://doi.org/10.1152/advan.00053.2002 |
url |
http://hdl.handle.net/10316/12639 https://doi.org/10.1152/advan.00053.2002 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Advances in Physiology Education. 27:1-4 (2003) 78-85 1043-4046 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.publisher.none.fl_str_mv |
The American Physiological Society |
publisher.none.fl_str_mv |
The American Physiological Society |
dc.source.none.fl_str_mv |
reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação instacron:RCAAP |
instname_str |
Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
instacron_str |
RCAAP |
institution |
RCAAP |
reponame_str |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
collection |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
repository.name.fl_str_mv |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
repository.mail.fl_str_mv |
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1799133707933581312 |