Survival of clinical and food isolates of Listeria monocytogenes through simulated gastrointestinal tract conditions
Autor(a) principal: | |
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Data de Publicação: | 2010 |
Outros Autores: | , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
Texto Completo: | http://hdl.handle.net/1822/33161 |
Resumo: | Twenty-seven strains of Listeria monocytogenes previously isolated from food (n=16) and human patients of listeriosis (n=11) were characterized and compared based on their ability to survive through the simulated gastrointestinal tract conditions. Cells were exposed (60 or 120min) to low pH in the presence of pepsin, to simulate the digestion in the stomach, and subsequently to bile salts to simulate the digestion in the small intestine (60 or 120min). Their survival was shown to be origin- (food and clinical) and strain dependent (p<0.001) and also significantly dependent on the imposed simulated gastric conditions (long vs. quick exposure) (p<0.001). In comparison to the food isolates, the clinical strains were in general more resistant and survived better to the two challenges imposed. Some of the tested strains, after the exposure to low pH in the presence of pepsin, became injured and subsequently more susceptible to the bile salts challenge. It was demonstrated that one of the most important natural barriers against foodborne pathogens might not be effective since it was shown that L. monocytogenes isolates that survived through the pH challenge were also able to survive the subsequent challenge to bile salts. |
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Survival of clinical and food isolates of Listeria monocytogenes through simulated gastrointestinal tract conditionsScience & TechnologyTwenty-seven strains of Listeria monocytogenes previously isolated from food (n=16) and human patients of listeriosis (n=11) were characterized and compared based on their ability to survive through the simulated gastrointestinal tract conditions. Cells were exposed (60 or 120min) to low pH in the presence of pepsin, to simulate the digestion in the stomach, and subsequently to bile salts to simulate the digestion in the small intestine (60 or 120min). Their survival was shown to be origin- (food and clinical) and strain dependent (p<0.001) and also significantly dependent on the imposed simulated gastric conditions (long vs. quick exposure) (p<0.001). In comparison to the food isolates, the clinical strains were in general more resistant and survived better to the two challenges imposed. Some of the tested strains, after the exposure to low pH in the presence of pepsin, became injured and subsequently more susceptible to the bile salts challenge. It was demonstrated that one of the most important natural barriers against foodborne pathogens might not be effective since it was shown that L. monocytogenes isolates that survived through the pH challenge were also able to survive the subsequent challenge to bile salts.This work was supported by CT project-2006-016264-Truefood. Traditional United Europe Food, and by FCT project-PTDC/AGR-ALI/64662/2006- Listeria monocytogenes in foods: contributing data for risk assessment.Mary Ann Liebert Inc.Universidade do MinhoRamalheira, R.Almeida, Marta A. S.Azeredo, JoanaBrandão, Teresa R. S.Almeida, G.Silva, J.Teixeira, P.20102010-01-01T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/1822/33161engRamalheira, R.; Almeida, Marta; Azeredo, Joana; Brandão, T. R. S.; Almeida, G.; Silva, J.; Teixeira, P., Survival of clinical and food isolates of Listeria monocytogenes through simulated gastrointestinal tract conditions. Foodborne Pathogens and Disease, 7(2), 121-128, 20101535-31411556-712510.1089/fpd.2009.031919785538info:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2023-07-21T12:04:47Zoai:repositorium.sdum.uminho.pt:1822/33161Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-19T18:55:06.836021Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse |
dc.title.none.fl_str_mv |
Survival of clinical and food isolates of Listeria monocytogenes through simulated gastrointestinal tract conditions |
title |
Survival of clinical and food isolates of Listeria monocytogenes through simulated gastrointestinal tract conditions |
spellingShingle |
Survival of clinical and food isolates of Listeria monocytogenes through simulated gastrointestinal tract conditions Ramalheira, R. Science & Technology |
title_short |
Survival of clinical and food isolates of Listeria monocytogenes through simulated gastrointestinal tract conditions |
title_full |
Survival of clinical and food isolates of Listeria monocytogenes through simulated gastrointestinal tract conditions |
title_fullStr |
Survival of clinical and food isolates of Listeria monocytogenes through simulated gastrointestinal tract conditions |
title_full_unstemmed |
Survival of clinical and food isolates of Listeria monocytogenes through simulated gastrointestinal tract conditions |
title_sort |
Survival of clinical and food isolates of Listeria monocytogenes through simulated gastrointestinal tract conditions |
author |
Ramalheira, R. |
author_facet |
Ramalheira, R. Almeida, Marta A. S. Azeredo, Joana Brandão, Teresa R. S. Almeida, G. Silva, J. Teixeira, P. |
author_role |
author |
author2 |
Almeida, Marta A. S. Azeredo, Joana Brandão, Teresa R. S. Almeida, G. Silva, J. Teixeira, P. |
author2_role |
author author author author author author |
dc.contributor.none.fl_str_mv |
Universidade do Minho |
dc.contributor.author.fl_str_mv |
Ramalheira, R. Almeida, Marta A. S. Azeredo, Joana Brandão, Teresa R. S. Almeida, G. Silva, J. Teixeira, P. |
dc.subject.por.fl_str_mv |
Science & Technology |
topic |
Science & Technology |
description |
Twenty-seven strains of Listeria monocytogenes previously isolated from food (n=16) and human patients of listeriosis (n=11) were characterized and compared based on their ability to survive through the simulated gastrointestinal tract conditions. Cells were exposed (60 or 120min) to low pH in the presence of pepsin, to simulate the digestion in the stomach, and subsequently to bile salts to simulate the digestion in the small intestine (60 or 120min). Their survival was shown to be origin- (food and clinical) and strain dependent (p<0.001) and also significantly dependent on the imposed simulated gastric conditions (long vs. quick exposure) (p<0.001). In comparison to the food isolates, the clinical strains were in general more resistant and survived better to the two challenges imposed. Some of the tested strains, after the exposure to low pH in the presence of pepsin, became injured and subsequently more susceptible to the bile salts challenge. It was demonstrated that one of the most important natural barriers against foodborne pathogens might not be effective since it was shown that L. monocytogenes isolates that survived through the pH challenge were also able to survive the subsequent challenge to bile salts. |
publishDate |
2010 |
dc.date.none.fl_str_mv |
2010 2010-01-01T00:00:00Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://hdl.handle.net/1822/33161 |
url |
http://hdl.handle.net/1822/33161 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Ramalheira, R.; Almeida, Marta; Azeredo, Joana; Brandão, T. R. S.; Almeida, G.; Silva, J.; Teixeira, P., Survival of clinical and food isolates of Listeria monocytogenes through simulated gastrointestinal tract conditions. Foodborne Pathogens and Disease, 7(2), 121-128, 2010 1535-3141 1556-7125 10.1089/fpd.2009.0319 19785538 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.publisher.none.fl_str_mv |
Mary Ann Liebert Inc. |
publisher.none.fl_str_mv |
Mary Ann Liebert Inc. |
dc.source.none.fl_str_mv |
reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação instacron:RCAAP |
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Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
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RCAAP |
institution |
RCAAP |
reponame_str |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
collection |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
repository.name.fl_str_mv |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
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1799132335440920576 |