Label-free quantum dot conjugates for human protein IL-2 based on molecularly imprinted polymers

Detalhes bibliográficos
Autor(a) principal: Piloto, Margarida
Data de Publicação: 2020
Outros Autores: Ribeiro, David S. M., Rodrigues, S. Sofia M., Santos, João L. M., Sales, M. G. F.
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
Texto Completo: http://hdl.handle.net/1822/62598
Resumo: Herein, the development of a fluorescent-based sensor by combining quantum dots (QDs) with molecularly-imprinted technology (MIP), intensively optimized to generate exceptional operating features is presented. This sensor is designed to target human interleukin-2 (IL-2) in synthetic human serum. IL-2 is a regulatory protein released as a triggered response from the immune system towards an inflammation. For this purpose, cadmium telluride (CdTe) QDs are prepared with 3-mercaptopropionic acid (MPA) and modified afterwards to produce an IL-2 imprinted polymer with methacrylic acid and N,N´-methylenebis(acrylamide), upon removal of the template under optimized conditions. During IL-2 rebinding, the fluorescence intensity of CdTe
id RCAP_159c1ae4cd5d4fb0b66895bc8053362e
oai_identifier_str oai:repositorium.sdum.uminho.pt:1822/62598
network_acronym_str RCAP
network_name_str Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
repository_id_str 7160
spelling Label-free quantum dot conjugates for human protein IL-2 based on molecularly imprinted polymersQuantum dotsmolecularly imprinted polymerconjugated-QDsproteininterleukin-2cancer biomarkerScience & TechnologyHerein, the development of a fluorescent-based sensor by combining quantum dots (QDs) with molecularly-imprinted technology (MIP), intensively optimized to generate exceptional operating features is presented. This sensor is designed to target human interleukin-2 (IL-2) in synthetic human serum. IL-2 is a regulatory protein released as a triggered response from the immune system towards an inflammation. For this purpose, cadmium telluride (CdTe) QDs are prepared with 3-mercaptopropionic acid (MPA) and modified afterwards to produce an IL-2 imprinted polymer with methacrylic acid and N,N´-methylenebis(acrylamide), upon removal of the template under optimized conditions. During IL-2 rebinding, the fluorescence intensity of CdTeThe authors acknowledge the financial support of European Research Council though the Starting Grant, 3P’s Starting Grant/ERC (GA 311086) and the financial support of Portugal 2020, FEDER and NORTE2020 through the project Strip2Sense (NORTE-01-0145-FEDER024358). AMLP also aknowledges Fundação para a Ciência e Tecnologia, I.P., for the grant reference SFRH/BPD/116067/2016.info:eu-repo/semantics/publishedVersionElsevierUniversidade do MinhoPiloto, MargaridaRibeiro, David S. M.Rodrigues, S. Sofia M.Santos, João L. M.Sales, M. G. F.2020-022020-02-01T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/1822/62598engPiloto, Margarida; David S. M. Ribeiro; S. Sofia M. Rodrigues; João L. M. Santos; Sales, M. G. F., Label-free quantum dot conjugates for human protein IL-2 based on molecularly imprinted polymers. Sensors and Actuators B-Chemical, 304(127343), 20200925-400510.1016/j.snb.2019.127343https://www.journals.elsevier.com/sensors-and-actuators-b-chemicalinfo:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2023-07-21T12:25:48Zoai:repositorium.sdum.uminho.pt:1822/62598Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-19T19:20:06.625716Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse
dc.title.none.fl_str_mv Label-free quantum dot conjugates for human protein IL-2 based on molecularly imprinted polymers
title Label-free quantum dot conjugates for human protein IL-2 based on molecularly imprinted polymers
spellingShingle Label-free quantum dot conjugates for human protein IL-2 based on molecularly imprinted polymers
Piloto, Margarida
Quantum dots
molecularly imprinted polymer
conjugated-QDs
protein
interleukin-2
cancer biomarker
Science & Technology
title_short Label-free quantum dot conjugates for human protein IL-2 based on molecularly imprinted polymers
title_full Label-free quantum dot conjugates for human protein IL-2 based on molecularly imprinted polymers
title_fullStr Label-free quantum dot conjugates for human protein IL-2 based on molecularly imprinted polymers
title_full_unstemmed Label-free quantum dot conjugates for human protein IL-2 based on molecularly imprinted polymers
title_sort Label-free quantum dot conjugates for human protein IL-2 based on molecularly imprinted polymers
author Piloto, Margarida
author_facet Piloto, Margarida
Ribeiro, David S. M.
Rodrigues, S. Sofia M.
Santos, João L. M.
Sales, M. G. F.
author_role author
author2 Ribeiro, David S. M.
Rodrigues, S. Sofia M.
Santos, João L. M.
Sales, M. G. F.
author2_role author
author
author
author
dc.contributor.none.fl_str_mv Universidade do Minho
dc.contributor.author.fl_str_mv Piloto, Margarida
Ribeiro, David S. M.
Rodrigues, S. Sofia M.
Santos, João L. M.
Sales, M. G. F.
dc.subject.por.fl_str_mv Quantum dots
molecularly imprinted polymer
conjugated-QDs
protein
interleukin-2
cancer biomarker
Science & Technology
topic Quantum dots
molecularly imprinted polymer
conjugated-QDs
protein
interleukin-2
cancer biomarker
Science & Technology
description Herein, the development of a fluorescent-based sensor by combining quantum dots (QDs) with molecularly-imprinted technology (MIP), intensively optimized to generate exceptional operating features is presented. This sensor is designed to target human interleukin-2 (IL-2) in synthetic human serum. IL-2 is a regulatory protein released as a triggered response from the immune system towards an inflammation. For this purpose, cadmium telluride (CdTe) QDs are prepared with 3-mercaptopropionic acid (MPA) and modified afterwards to produce an IL-2 imprinted polymer with methacrylic acid and N,N´-methylenebis(acrylamide), upon removal of the template under optimized conditions. During IL-2 rebinding, the fluorescence intensity of CdTe
publishDate 2020
dc.date.none.fl_str_mv 2020-02
2020-02-01T00:00:00Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://hdl.handle.net/1822/62598
url http://hdl.handle.net/1822/62598
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv Piloto, Margarida; David S. M. Ribeiro; S. Sofia M. Rodrigues; João L. M. Santos; Sales, M. G. F., Label-free quantum dot conjugates for human protein IL-2 based on molecularly imprinted polymers. Sensors and Actuators B-Chemical, 304(127343), 2020
0925-4005
10.1016/j.snb.2019.127343
https://www.journals.elsevier.com/sensors-and-actuators-b-chemical
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Elsevier
publisher.none.fl_str_mv Elsevier
dc.source.none.fl_str_mv reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron:RCAAP
instname_str Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron_str RCAAP
institution RCAAP
reponame_str Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
collection Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
repository.name.fl_str_mv Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
repository.mail.fl_str_mv
_version_ 1799132662711975936

Registros relacionados