Label-free quantum dot conjugates for human protein IL-2 based on molecularly imprinted polymers
Autor(a) principal: | |
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Data de Publicação: | 2020 |
Outros Autores: | , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
Texto Completo: | http://hdl.handle.net/1822/62598 |
Resumo: | Herein, the development of a fluorescent-based sensor by combining quantum dots (QDs) with molecularly-imprinted technology (MIP), intensively optimized to generate exceptional operating features is presented. This sensor is designed to target human interleukin-2 (IL-2) in synthetic human serum. IL-2 is a regulatory protein released as a triggered response from the immune system towards an inflammation. For this purpose, cadmium telluride (CdTe) QDs are prepared with 3-mercaptopropionic acid (MPA) and modified afterwards to produce an IL-2 imprinted polymer with methacrylic acid and N,N´-methylenebis(acrylamide), upon removal of the template under optimized conditions. During IL-2 rebinding, the fluorescence intensity of CdTe |
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Label-free quantum dot conjugates for human protein IL-2 based on molecularly imprinted polymersQuantum dotsmolecularly imprinted polymerconjugated-QDsproteininterleukin-2cancer biomarkerScience & TechnologyHerein, the development of a fluorescent-based sensor by combining quantum dots (QDs) with molecularly-imprinted technology (MIP), intensively optimized to generate exceptional operating features is presented. This sensor is designed to target human interleukin-2 (IL-2) in synthetic human serum. IL-2 is a regulatory protein released as a triggered response from the immune system towards an inflammation. For this purpose, cadmium telluride (CdTe) QDs are prepared with 3-mercaptopropionic acid (MPA) and modified afterwards to produce an IL-2 imprinted polymer with methacrylic acid and N,N´-methylenebis(acrylamide), upon removal of the template under optimized conditions. During IL-2 rebinding, the fluorescence intensity of CdTeThe authors acknowledge the financial support of European Research Council though the Starting Grant, 3P’s Starting Grant/ERC (GA 311086) and the financial support of Portugal 2020, FEDER and NORTE2020 through the project Strip2Sense (NORTE-01-0145-FEDER024358). AMLP also aknowledges Fundação para a Ciência e Tecnologia, I.P., for the grant reference SFRH/BPD/116067/2016.info:eu-repo/semantics/publishedVersionElsevierUniversidade do MinhoPiloto, MargaridaRibeiro, David S. M.Rodrigues, S. Sofia M.Santos, João L. M.Sales, M. G. F.2020-022020-02-01T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/1822/62598engPiloto, Margarida; David S. M. Ribeiro; S. Sofia M. Rodrigues; João L. M. Santos; Sales, M. G. F., Label-free quantum dot conjugates for human protein IL-2 based on molecularly imprinted polymers. Sensors and Actuators B-Chemical, 304(127343), 20200925-400510.1016/j.snb.2019.127343https://www.journals.elsevier.com/sensors-and-actuators-b-chemicalinfo:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2023-07-21T12:25:48Zoai:repositorium.sdum.uminho.pt:1822/62598Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-19T19:20:06.625716Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse |
dc.title.none.fl_str_mv |
Label-free quantum dot conjugates for human protein IL-2 based on molecularly imprinted polymers |
title |
Label-free quantum dot conjugates for human protein IL-2 based on molecularly imprinted polymers |
spellingShingle |
Label-free quantum dot conjugates for human protein IL-2 based on molecularly imprinted polymers Piloto, Margarida Quantum dots molecularly imprinted polymer conjugated-QDs protein interleukin-2 cancer biomarker Science & Technology |
title_short |
Label-free quantum dot conjugates for human protein IL-2 based on molecularly imprinted polymers |
title_full |
Label-free quantum dot conjugates for human protein IL-2 based on molecularly imprinted polymers |
title_fullStr |
Label-free quantum dot conjugates for human protein IL-2 based on molecularly imprinted polymers |
title_full_unstemmed |
Label-free quantum dot conjugates for human protein IL-2 based on molecularly imprinted polymers |
title_sort |
Label-free quantum dot conjugates for human protein IL-2 based on molecularly imprinted polymers |
author |
Piloto, Margarida |
author_facet |
Piloto, Margarida Ribeiro, David S. M. Rodrigues, S. Sofia M. Santos, João L. M. Sales, M. G. F. |
author_role |
author |
author2 |
Ribeiro, David S. M. Rodrigues, S. Sofia M. Santos, João L. M. Sales, M. G. F. |
author2_role |
author author author author |
dc.contributor.none.fl_str_mv |
Universidade do Minho |
dc.contributor.author.fl_str_mv |
Piloto, Margarida Ribeiro, David S. M. Rodrigues, S. Sofia M. Santos, João L. M. Sales, M. G. F. |
dc.subject.por.fl_str_mv |
Quantum dots molecularly imprinted polymer conjugated-QDs protein interleukin-2 cancer biomarker Science & Technology |
topic |
Quantum dots molecularly imprinted polymer conjugated-QDs protein interleukin-2 cancer biomarker Science & Technology |
description |
Herein, the development of a fluorescent-based sensor by combining quantum dots (QDs) with molecularly-imprinted technology (MIP), intensively optimized to generate exceptional operating features is presented. This sensor is designed to target human interleukin-2 (IL-2) in synthetic human serum. IL-2 is a regulatory protein released as a triggered response from the immune system towards an inflammation. For this purpose, cadmium telluride (CdTe) QDs are prepared with 3-mercaptopropionic acid (MPA) and modified afterwards to produce an IL-2 imprinted polymer with methacrylic acid and N,N´-methylenebis(acrylamide), upon removal of the template under optimized conditions. During IL-2 rebinding, the fluorescence intensity of CdTe |
publishDate |
2020 |
dc.date.none.fl_str_mv |
2020-02 2020-02-01T00:00:00Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://hdl.handle.net/1822/62598 |
url |
http://hdl.handle.net/1822/62598 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Piloto, Margarida; David S. M. Ribeiro; S. Sofia M. Rodrigues; João L. M. Santos; Sales, M. G. F., Label-free quantum dot conjugates for human protein IL-2 based on molecularly imprinted polymers. Sensors and Actuators B-Chemical, 304(127343), 2020 0925-4005 10.1016/j.snb.2019.127343 https://www.journals.elsevier.com/sensors-and-actuators-b-chemical |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.publisher.none.fl_str_mv |
Elsevier |
publisher.none.fl_str_mv |
Elsevier |
dc.source.none.fl_str_mv |
reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação instacron:RCAAP |
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Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
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RCAAP |
institution |
RCAAP |
reponame_str |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
collection |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
repository.name.fl_str_mv |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
repository.mail.fl_str_mv |
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1799132662711975936 |