Independent recruitment of Igh alleles in V(D)J recombination

Detalhes bibliográficos
Autor(a) principal: Alves-Pereira, Clara F.
Data de Publicação: 2014
Outros Autores: de Freitas, Raquel, Lopes, Telma, Gardner, Rui, Marta, Filipa, Vieira, Paulo, Barreto, Vasco M.
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
Texto Completo: http://hdl.handle.net/10400.7/510
Resumo: How the vast majority of B cells express only one of the two alleles at their immunoglobulin loci remains a biological puzzle. Here, in mice reconstituted with a single haematopoietic stem cell, we demonstrate that each of the two immunoglobulin heavy chain (Igh) alleles has a similar probability to be the first to undergo V(H) to DJ(H) rearrangement. We also observe this similar probability in clones from multipotent and common lymphoid precursors. The extreme biases in the expression of the alleles that we find in more differentiated subsets are mostly due to constraints imposed by early rearrangements. Our data demonstrate that each of the two Igh alleles in a B cell behaves independently of the other, up to the moment when a successful rearrangement in one allele triggers a feedback mechanism that prevents further recombination.
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spelling Independent recruitment of Igh alleles in V(D)J recombinationBase SequenceCell DifferentiationClone CellsEpigenesis, GeneticGene Expression RegulationBiological sciencesImmunologyV(D)J RecombinationAllelesHow the vast majority of B cells express only one of the two alleles at their immunoglobulin loci remains a biological puzzle. Here, in mice reconstituted with a single haematopoietic stem cell, we demonstrate that each of the two immunoglobulin heavy chain (Igh) alleles has a similar probability to be the first to undergo V(H) to DJ(H) rearrangement. We also observe this similar probability in clones from multipotent and common lymphoid precursors. The extreme biases in the expression of the alleles that we find in more differentiated subsets are mostly due to constraints imposed by early rearrangements. Our data demonstrate that each of the two Igh alleles in a B cell behaves independently of the other, up to the moment when a successful rearrangement in one allele triggers a feedback mechanism that prevents further recombination.FCT fellowship: (SFRH/BD/33217/2007).Nature Publishing GroupARCAAlves-Pereira, Clara F.de Freitas, RaquelLopes, TelmaGardner, RuiMarta, FilipaVieira, PauloBarreto, Vasco M.2015-11-19T13:12:06Z2014-12-172014-12-17T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/10400.7/510engPereira, C. F. A. et al. Independent recruitment of Igh alleles in V(D)J recombination. Nat. Commun. 5:5623 doi: 10.1038/ncomms6623 (2014).10.1038/ncomms6623info:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2022-11-29T14:34:53Zoai:arca.igc.gulbenkian.pt:10400.7/510Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-19T16:11:46.562778Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse
dc.title.none.fl_str_mv Independent recruitment of Igh alleles in V(D)J recombination
title Independent recruitment of Igh alleles in V(D)J recombination
spellingShingle Independent recruitment of Igh alleles in V(D)J recombination
Alves-Pereira, Clara F.
Base Sequence
Cell Differentiation
Clone Cells
Epigenesis, Genetic
Gene Expression Regulation
Biological sciences
Immunology
V(D)J Recombination
Alleles
title_short Independent recruitment of Igh alleles in V(D)J recombination
title_full Independent recruitment of Igh alleles in V(D)J recombination
title_fullStr Independent recruitment of Igh alleles in V(D)J recombination
title_full_unstemmed Independent recruitment of Igh alleles in V(D)J recombination
title_sort Independent recruitment of Igh alleles in V(D)J recombination
author Alves-Pereira, Clara F.
author_facet Alves-Pereira, Clara F.
de Freitas, Raquel
Lopes, Telma
Gardner, Rui
Marta, Filipa
Vieira, Paulo
Barreto, Vasco M.
author_role author
author2 de Freitas, Raquel
Lopes, Telma
Gardner, Rui
Marta, Filipa
Vieira, Paulo
Barreto, Vasco M.
author2_role author
author
author
author
author
author
dc.contributor.none.fl_str_mv ARCA
dc.contributor.author.fl_str_mv Alves-Pereira, Clara F.
de Freitas, Raquel
Lopes, Telma
Gardner, Rui
Marta, Filipa
Vieira, Paulo
Barreto, Vasco M.
dc.subject.por.fl_str_mv Base Sequence
Cell Differentiation
Clone Cells
Epigenesis, Genetic
Gene Expression Regulation
Biological sciences
Immunology
V(D)J Recombination
Alleles
topic Base Sequence
Cell Differentiation
Clone Cells
Epigenesis, Genetic
Gene Expression Regulation
Biological sciences
Immunology
V(D)J Recombination
Alleles
description How the vast majority of B cells express only one of the two alleles at their immunoglobulin loci remains a biological puzzle. Here, in mice reconstituted with a single haematopoietic stem cell, we demonstrate that each of the two immunoglobulin heavy chain (Igh) alleles has a similar probability to be the first to undergo V(H) to DJ(H) rearrangement. We also observe this similar probability in clones from multipotent and common lymphoid precursors. The extreme biases in the expression of the alleles that we find in more differentiated subsets are mostly due to constraints imposed by early rearrangements. Our data demonstrate that each of the two Igh alleles in a B cell behaves independently of the other, up to the moment when a successful rearrangement in one allele triggers a feedback mechanism that prevents further recombination.
publishDate 2014
dc.date.none.fl_str_mv 2014-12-17
2014-12-17T00:00:00Z
2015-11-19T13:12:06Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://hdl.handle.net/10400.7/510
url http://hdl.handle.net/10400.7/510
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv Pereira, C. F. A. et al. Independent recruitment of Igh alleles in V(D)J recombination. Nat. Commun. 5:5623 doi: 10.1038/ncomms6623 (2014).
10.1038/ncomms6623
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Nature Publishing Group
publisher.none.fl_str_mv Nature Publishing Group
dc.source.none.fl_str_mv reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron:RCAAP
instname_str Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron_str RCAAP
institution RCAAP
reponame_str Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
collection Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
repository.name.fl_str_mv Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
repository.mail.fl_str_mv
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