Independent recruitment of Igh alleles in V(D)J recombination
Autor(a) principal: | |
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Data de Publicação: | 2014 |
Outros Autores: | , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
Texto Completo: | http://hdl.handle.net/10400.7/510 |
Resumo: | How the vast majority of B cells express only one of the two alleles at their immunoglobulin loci remains a biological puzzle. Here, in mice reconstituted with a single haematopoietic stem cell, we demonstrate that each of the two immunoglobulin heavy chain (Igh) alleles has a similar probability to be the first to undergo V(H) to DJ(H) rearrangement. We also observe this similar probability in clones from multipotent and common lymphoid precursors. The extreme biases in the expression of the alleles that we find in more differentiated subsets are mostly due to constraints imposed by early rearrangements. Our data demonstrate that each of the two Igh alleles in a B cell behaves independently of the other, up to the moment when a successful rearrangement in one allele triggers a feedback mechanism that prevents further recombination. |
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Independent recruitment of Igh alleles in V(D)J recombinationBase SequenceCell DifferentiationClone CellsEpigenesis, GeneticGene Expression RegulationBiological sciencesImmunologyV(D)J RecombinationAllelesHow the vast majority of B cells express only one of the two alleles at their immunoglobulin loci remains a biological puzzle. Here, in mice reconstituted with a single haematopoietic stem cell, we demonstrate that each of the two immunoglobulin heavy chain (Igh) alleles has a similar probability to be the first to undergo V(H) to DJ(H) rearrangement. We also observe this similar probability in clones from multipotent and common lymphoid precursors. The extreme biases in the expression of the alleles that we find in more differentiated subsets are mostly due to constraints imposed by early rearrangements. Our data demonstrate that each of the two Igh alleles in a B cell behaves independently of the other, up to the moment when a successful rearrangement in one allele triggers a feedback mechanism that prevents further recombination.FCT fellowship: (SFRH/BD/33217/2007).Nature Publishing GroupARCAAlves-Pereira, Clara F.de Freitas, RaquelLopes, TelmaGardner, RuiMarta, FilipaVieira, PauloBarreto, Vasco M.2015-11-19T13:12:06Z2014-12-172014-12-17T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/10400.7/510engPereira, C. F. A. et al. Independent recruitment of Igh alleles in V(D)J recombination. Nat. Commun. 5:5623 doi: 10.1038/ncomms6623 (2014).10.1038/ncomms6623info:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2022-11-29T14:34:53Zoai:arca.igc.gulbenkian.pt:10400.7/510Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-19T16:11:46.562778Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse |
dc.title.none.fl_str_mv |
Independent recruitment of Igh alleles in V(D)J recombination |
title |
Independent recruitment of Igh alleles in V(D)J recombination |
spellingShingle |
Independent recruitment of Igh alleles in V(D)J recombination Alves-Pereira, Clara F. Base Sequence Cell Differentiation Clone Cells Epigenesis, Genetic Gene Expression Regulation Biological sciences Immunology V(D)J Recombination Alleles |
title_short |
Independent recruitment of Igh alleles in V(D)J recombination |
title_full |
Independent recruitment of Igh alleles in V(D)J recombination |
title_fullStr |
Independent recruitment of Igh alleles in V(D)J recombination |
title_full_unstemmed |
Independent recruitment of Igh alleles in V(D)J recombination |
title_sort |
Independent recruitment of Igh alleles in V(D)J recombination |
author |
Alves-Pereira, Clara F. |
author_facet |
Alves-Pereira, Clara F. de Freitas, Raquel Lopes, Telma Gardner, Rui Marta, Filipa Vieira, Paulo Barreto, Vasco M. |
author_role |
author |
author2 |
de Freitas, Raquel Lopes, Telma Gardner, Rui Marta, Filipa Vieira, Paulo Barreto, Vasco M. |
author2_role |
author author author author author author |
dc.contributor.none.fl_str_mv |
ARCA |
dc.contributor.author.fl_str_mv |
Alves-Pereira, Clara F. de Freitas, Raquel Lopes, Telma Gardner, Rui Marta, Filipa Vieira, Paulo Barreto, Vasco M. |
dc.subject.por.fl_str_mv |
Base Sequence Cell Differentiation Clone Cells Epigenesis, Genetic Gene Expression Regulation Biological sciences Immunology V(D)J Recombination Alleles |
topic |
Base Sequence Cell Differentiation Clone Cells Epigenesis, Genetic Gene Expression Regulation Biological sciences Immunology V(D)J Recombination Alleles |
description |
How the vast majority of B cells express only one of the two alleles at their immunoglobulin loci remains a biological puzzle. Here, in mice reconstituted with a single haematopoietic stem cell, we demonstrate that each of the two immunoglobulin heavy chain (Igh) alleles has a similar probability to be the first to undergo V(H) to DJ(H) rearrangement. We also observe this similar probability in clones from multipotent and common lymphoid precursors. The extreme biases in the expression of the alleles that we find in more differentiated subsets are mostly due to constraints imposed by early rearrangements. Our data demonstrate that each of the two Igh alleles in a B cell behaves independently of the other, up to the moment when a successful rearrangement in one allele triggers a feedback mechanism that prevents further recombination. |
publishDate |
2014 |
dc.date.none.fl_str_mv |
2014-12-17 2014-12-17T00:00:00Z 2015-11-19T13:12:06Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://hdl.handle.net/10400.7/510 |
url |
http://hdl.handle.net/10400.7/510 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Pereira, C. F. A. et al. Independent recruitment of Igh alleles in V(D)J recombination. Nat. Commun. 5:5623 doi: 10.1038/ncomms6623 (2014). 10.1038/ncomms6623 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.publisher.none.fl_str_mv |
Nature Publishing Group |
publisher.none.fl_str_mv |
Nature Publishing Group |
dc.source.none.fl_str_mv |
reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação instacron:RCAAP |
instname_str |
Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
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RCAAP |
institution |
RCAAP |
reponame_str |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
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Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
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Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
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1799130573000671232 |