Towards non-invasive intravital microscopy: advantages of using the ear lobe instead of the cremaster muscle

Detalhes bibliográficos
Autor(a) principal: Mota-Silva, Iara
Data de Publicação: 2023
Outros Autores: Castanho, Miguel A. R. B., Silva-Herdade, Ana S.
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
Texto Completo: http://hdl.handle.net/10451/57606
Resumo: © 2023 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
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spelling Towards non-invasive intravital microscopy: advantages of using the ear lobe instead of the cremaster muscleIntravital microscopyLeukostasisInflammationMicrocirculation© 2023 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).Inflammation is essential in the protection of the organism and wound repair, but in cases of chronic inflammation can also cause microvasculature deterioration. Thus, inflammation monitorization studies are important to test potential therapeutics. The intravital microscopy (IVM) technique monitors leukocyte trafficking in vivo, being a commonly used procedure to report systemic conditions. Although the cremaster muscle, an established protocol for IVM, may affect the hemodynamics because of its surgical preparation, only male animals are used, and longitudinal studies over time are not feasible. Thinking how this impacts future studies, our aim is to understand if the IVM technique can be successfully performed using the ear lobe instead of the cremaster muscle. Elevated IL-1β plasmatic concentrations confirmed the systemic inflammation developed in a diabetic animal model, while the elevated number of adherent and rolling leukocytes in the ear lobe allowed for the same conclusion. Thus, this study demonstrates that albeit its thickness, the ear lobe protocol for IVM is efficient, non-invasive, more reliable, cost-effective and timesaving.The project leading to these results has received funding from “la Caixa” Foundation under the project code HR21-00605, ID 100010434 and FCT, I.P under the agreement LCF/PR/HP21/52310015.MDPIRepositório da Universidade de LisboaMota-Silva, IaraCastanho, Miguel A. R. B.Silva-Herdade, Ana S.2023-05-25T10:55:49Z20232023-01-01T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/10451/57606engLife 2023, 13(4), 88710.3390/life130408872075-1729info:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2023-11-08T17:06:12Zoai:repositorio.ul.pt:10451/57606Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-19T22:08:05.820534Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse
dc.title.none.fl_str_mv Towards non-invasive intravital microscopy: advantages of using the ear lobe instead of the cremaster muscle
title Towards non-invasive intravital microscopy: advantages of using the ear lobe instead of the cremaster muscle
spellingShingle Towards non-invasive intravital microscopy: advantages of using the ear lobe instead of the cremaster muscle
Mota-Silva, Iara
Intravital microscopy
Leukostasis
Inflammation
Microcirculation
title_short Towards non-invasive intravital microscopy: advantages of using the ear lobe instead of the cremaster muscle
title_full Towards non-invasive intravital microscopy: advantages of using the ear lobe instead of the cremaster muscle
title_fullStr Towards non-invasive intravital microscopy: advantages of using the ear lobe instead of the cremaster muscle
title_full_unstemmed Towards non-invasive intravital microscopy: advantages of using the ear lobe instead of the cremaster muscle
title_sort Towards non-invasive intravital microscopy: advantages of using the ear lobe instead of the cremaster muscle
author Mota-Silva, Iara
author_facet Mota-Silva, Iara
Castanho, Miguel A. R. B.
Silva-Herdade, Ana S.
author_role author
author2 Castanho, Miguel A. R. B.
Silva-Herdade, Ana S.
author2_role author
author
dc.contributor.none.fl_str_mv Repositório da Universidade de Lisboa
dc.contributor.author.fl_str_mv Mota-Silva, Iara
Castanho, Miguel A. R. B.
Silva-Herdade, Ana S.
dc.subject.por.fl_str_mv Intravital microscopy
Leukostasis
Inflammation
Microcirculation
topic Intravital microscopy
Leukostasis
Inflammation
Microcirculation
description © 2023 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
publishDate 2023
dc.date.none.fl_str_mv 2023-05-25T10:55:49Z
2023
2023-01-01T00:00:00Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
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dc.identifier.uri.fl_str_mv http://hdl.handle.net/10451/57606
url http://hdl.handle.net/10451/57606
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv Life 2023, 13(4), 887
10.3390/life13040887
2075-1729
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publisher.none.fl_str_mv MDPI
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