Fractionation and recovery of whey proteins by hydrophobic interaction chromatography

Detalhes bibliográficos
Autor(a) principal: Santos, Maria João
Data de Publicação: 2011
Outros Autores: Teixeira, J. A., Rodrigues, L. R.
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
Texto Completo: http://hdl.handle.net/1822/11930
Resumo: A method for the recovery and fractionation of whey proteins from a whey protein concentrate (80%, w/w) by hydrophobic interaction chromatography is proposed. Standard proteins and WPC 80 dissolved in phosphate buffer with ammonium sulfate 1M were loaded in a HiPrep Octyl Sepharose FF column coupled to a fast protein liquid chromatography (FPLC) system and eluted by decreasing the ionic strength of the buffer using a salt gradient. The results showed that the most hydrophobic protein from whey is -lactalbumin and the less hydrophobic is lactoferrin. It was possible to recover 45.2% of -lactoglobulin using the HiPrep Octyl Sepharose FF column from the whey protein concentrate mixture with 99.6% purity on total protein basis
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spelling Fractionation and recovery of whey proteins by hydrophobic interaction chromatographyHydrophobic interaction chromatographywhey proteinsb-lactoglobulinrecovery & isolationRecoveryIsolationβ-LactoglobulinScience & TechnologyA method for the recovery and fractionation of whey proteins from a whey protein concentrate (80%, w/w) by hydrophobic interaction chromatography is proposed. Standard proteins and WPC 80 dissolved in phosphate buffer with ammonium sulfate 1M were loaded in a HiPrep Octyl Sepharose FF column coupled to a fast protein liquid chromatography (FPLC) system and eluted by decreasing the ionic strength of the buffer using a salt gradient. The results showed that the most hydrophobic protein from whey is -lactalbumin and the less hydrophobic is lactoferrin. It was possible to recover 45.2% of -lactoglobulin using the HiPrep Octyl Sepharose FF column from the whey protein concentrate mixture with 99.6% purity on total protein basisElsevierUniversidade do MinhoSantos, Maria JoãoTeixeira, J. A.Rodrigues, L. R.2011-03-222011-03-22T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/1822/11930eng"Journal of Chromatography B". ISSN 1570-0232. 879 (2011) 475-479.1570-023210.1016/j.jchromb.2011.01.00321292570info:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2023-07-21T11:56:29Zoai:repositorium.sdum.uminho.pt:1822/11930Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-19T18:46:05.166736Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse
dc.title.none.fl_str_mv Fractionation and recovery of whey proteins by hydrophobic interaction chromatography
title Fractionation and recovery of whey proteins by hydrophobic interaction chromatography
spellingShingle Fractionation and recovery of whey proteins by hydrophobic interaction chromatography
Santos, Maria João
Hydrophobic interaction chromatography
whey proteins
b-lactoglobulin
recovery & isolation
Recovery
Isolation
β-Lactoglobulin
Science & Technology
title_short Fractionation and recovery of whey proteins by hydrophobic interaction chromatography
title_full Fractionation and recovery of whey proteins by hydrophobic interaction chromatography
title_fullStr Fractionation and recovery of whey proteins by hydrophobic interaction chromatography
title_full_unstemmed Fractionation and recovery of whey proteins by hydrophobic interaction chromatography
title_sort Fractionation and recovery of whey proteins by hydrophobic interaction chromatography
author Santos, Maria João
author_facet Santos, Maria João
Teixeira, J. A.
Rodrigues, L. R.
author_role author
author2 Teixeira, J. A.
Rodrigues, L. R.
author2_role author
author
dc.contributor.none.fl_str_mv Universidade do Minho
dc.contributor.author.fl_str_mv Santos, Maria João
Teixeira, J. A.
Rodrigues, L. R.
dc.subject.por.fl_str_mv Hydrophobic interaction chromatography
whey proteins
b-lactoglobulin
recovery & isolation
Recovery
Isolation
β-Lactoglobulin
Science & Technology
topic Hydrophobic interaction chromatography
whey proteins
b-lactoglobulin
recovery & isolation
Recovery
Isolation
β-Lactoglobulin
Science & Technology
description A method for the recovery and fractionation of whey proteins from a whey protein concentrate (80%, w/w) by hydrophobic interaction chromatography is proposed. Standard proteins and WPC 80 dissolved in phosphate buffer with ammonium sulfate 1M were loaded in a HiPrep Octyl Sepharose FF column coupled to a fast protein liquid chromatography (FPLC) system and eluted by decreasing the ionic strength of the buffer using a salt gradient. The results showed that the most hydrophobic protein from whey is -lactalbumin and the less hydrophobic is lactoferrin. It was possible to recover 45.2% of -lactoglobulin using the HiPrep Octyl Sepharose FF column from the whey protein concentrate mixture with 99.6% purity on total protein basis
publishDate 2011
dc.date.none.fl_str_mv 2011-03-22
2011-03-22T00:00:00Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://hdl.handle.net/1822/11930
url http://hdl.handle.net/1822/11930
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv "Journal of Chromatography B". ISSN 1570-0232. 879 (2011) 475-479.
1570-0232
10.1016/j.jchromb.2011.01.003
21292570
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Elsevier
publisher.none.fl_str_mv Elsevier
dc.source.none.fl_str_mv reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron:RCAAP
instname_str Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron_str RCAAP
institution RCAAP
reponame_str Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
collection Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
repository.name.fl_str_mv Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
repository.mail.fl_str_mv
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