Partitioning and separation of α-lactalbumin and β-lactoglobulin in polyethylene glycol/ammonium sulphate aqueous two-phase systems

• Autor(a) principal: Rodrigues, L. R.
• Data de Publicação: 2001
• Outros Autores: Venâncio, Armando, Teixeira, J. A.
• Tipo de documento: Artigo
• Idioma: eng
• Título da fonte: Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
• Texto Completo: http://hdl.handle.net/1822/932
• topic: Ammonium sulphate; α-lactalbumin; β-lactoglobulin; PEG; Whey; beta-lactoglobulin; Science & Technology

The partition behaviour of α-lactalbumin (αla) and β-lactoglobulin (βlg) on PEG/(NH4)2SO4 system was studied. For purified proteins, a partition coefficient of 12.8 for αla and 0.34 for βlg, with mass recovery yields of 96.7% for αla in the upper phase and 83.8% for βlg in the lower phase was obtained, in 18% (w/w) PEG 900/14% (w/w) (NH4)2SO4 system, at pH 7. PEG/(NH4)2SO4 system was an economical alternative for the recovery and separation of the two proteins in cheese whey, allowing a 50% reduction in costs. An efficient and inexpensive separation of both proteins in cheese whey could be achieved, by using 16% (w/w) PEG 900/15% (w/w) (NH4)2SO4, at pH 7.5.