Phase topology and percolation in two-component lipid bilayers: A Monte Carlo approach
Autor(a) principal: | |
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Data de Publicação: | 1997 |
Outros Autores: | , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
Texto Completo: | http://hdl.handle.net/10400.1/11113 |
Resumo: | Monte Carlo simulations of fluorescence recovery after photobleaching (FRAP) experiments on two-component lipid bilayers systems in the solid-fluid phase coexistence region were carried out to study the geometry and size of fluid domains in these bilayers. The gel phase was simulated by superposable elliptical domains, which were either of predetermined dimensions, increasing in number with increasing gel phase fraction, or of predetermined number, increasing in dimensions with increasing gel phase fraction. The simulations were done from two perspectives: 1) a time-independent analysis of fractional fluorescence recovery as a function of fractional fluid phase in the system; and 2) a time-dependent analysis of fractional fluorescence recovery as a function of time al a given fraction of fluid phase in the system. The time-dependent simulations result in recovery curves that are directly comparable to experimental FRAP curves and provide topological and geometrical models for the coexisting phases that are consistent with the experimental result. |
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Phase topology and percolation in two-component lipid bilayers: A Monte Carlo approach2-Phase phosphatidylcholine bilayerLateral diffusionTranslational diffusionPhospholipid membranesBinary-mixtures2-ComponentFluidConnectivityObstaclesCholesterolMonte Carlo simulations of fluorescence recovery after photobleaching (FRAP) experiments on two-component lipid bilayers systems in the solid-fluid phase coexistence region were carried out to study the geometry and size of fluid domains in these bilayers. The gel phase was simulated by superposable elliptical domains, which were either of predetermined dimensions, increasing in number with increasing gel phase fraction, or of predetermined number, increasing in dimensions with increasing gel phase fraction. The simulations were done from two perspectives: 1) a time-independent analysis of fractional fluorescence recovery as a function of fractional fluid phase in the system; and 2) a time-dependent analysis of fractional fluorescence recovery as a function of time al a given fraction of fluid phase in the system. The time-dependent simulations result in recovery curves that are directly comparable to experimental FRAP curves and provide topological and geometrical models for the coexisting phases that are consistent with the experimental result.Biophysical SocietySapientiaCoelho, FPVaz, WinchilMelo, E2018-12-07T14:52:32Z1997-041997-04-01T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/10400.1/11113eng0006-349510.1016/S0006-3495(97)78798-8info:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2023-07-24T10:22:51Zoai:sapientia.ualg.pt:10400.1/11113Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-19T20:02:38.322226Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse |
dc.title.none.fl_str_mv |
Phase topology and percolation in two-component lipid bilayers: A Monte Carlo approach |
title |
Phase topology and percolation in two-component lipid bilayers: A Monte Carlo approach |
spellingShingle |
Phase topology and percolation in two-component lipid bilayers: A Monte Carlo approach Coelho, FP 2-Phase phosphatidylcholine bilayer Lateral diffusion Translational diffusion Phospholipid membranes Binary-mixtures 2-Component Fluid Connectivity Obstacles Cholesterol |
title_short |
Phase topology and percolation in two-component lipid bilayers: A Monte Carlo approach |
title_full |
Phase topology and percolation in two-component lipid bilayers: A Monte Carlo approach |
title_fullStr |
Phase topology and percolation in two-component lipid bilayers: A Monte Carlo approach |
title_full_unstemmed |
Phase topology and percolation in two-component lipid bilayers: A Monte Carlo approach |
title_sort |
Phase topology and percolation in two-component lipid bilayers: A Monte Carlo approach |
author |
Coelho, FP |
author_facet |
Coelho, FP Vaz, Winchil Melo, E |
author_role |
author |
author2 |
Vaz, Winchil Melo, E |
author2_role |
author author |
dc.contributor.none.fl_str_mv |
Sapientia |
dc.contributor.author.fl_str_mv |
Coelho, FP Vaz, Winchil Melo, E |
dc.subject.por.fl_str_mv |
2-Phase phosphatidylcholine bilayer Lateral diffusion Translational diffusion Phospholipid membranes Binary-mixtures 2-Component Fluid Connectivity Obstacles Cholesterol |
topic |
2-Phase phosphatidylcholine bilayer Lateral diffusion Translational diffusion Phospholipid membranes Binary-mixtures 2-Component Fluid Connectivity Obstacles Cholesterol |
description |
Monte Carlo simulations of fluorescence recovery after photobleaching (FRAP) experiments on two-component lipid bilayers systems in the solid-fluid phase coexistence region were carried out to study the geometry and size of fluid domains in these bilayers. The gel phase was simulated by superposable elliptical domains, which were either of predetermined dimensions, increasing in number with increasing gel phase fraction, or of predetermined number, increasing in dimensions with increasing gel phase fraction. The simulations were done from two perspectives: 1) a time-independent analysis of fractional fluorescence recovery as a function of fractional fluid phase in the system; and 2) a time-dependent analysis of fractional fluorescence recovery as a function of time al a given fraction of fluid phase in the system. The time-dependent simulations result in recovery curves that are directly comparable to experimental FRAP curves and provide topological and geometrical models for the coexisting phases that are consistent with the experimental result. |
publishDate |
1997 |
dc.date.none.fl_str_mv |
1997-04 1997-04-01T00:00:00Z 2018-12-07T14:52:32Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://hdl.handle.net/10400.1/11113 |
url |
http://hdl.handle.net/10400.1/11113 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
0006-3495 10.1016/S0006-3495(97)78798-8 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.publisher.none.fl_str_mv |
Biophysical Society |
publisher.none.fl_str_mv |
Biophysical Society |
dc.source.none.fl_str_mv |
reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação instacron:RCAAP |
instname_str |
Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
instacron_str |
RCAAP |
institution |
RCAAP |
reponame_str |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
collection |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
repository.name.fl_str_mv |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
repository.mail.fl_str_mv |
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1799133260815532032 |