An internal promoter drives the expression of a truncated form of ccc1 capable of protecting yeast from iron toxicity

Detalhes bibliográficos
Autor(a) principal: Amaral, Catarina
Data de Publicação: 2021
Outros Autores: Vicente, Cristina Teixeira, Caetano, Soraia Marques, Gaspar-Cordeiro, Ana, Yang, Yang, Cloetens, Peter, Romão, Célia V., Rodrigues-Pousada, Claudina, Pimentel, Catarina
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
Texto Completo: http://hdl.handle.net/10362/128804
Resumo: Funding Information: This work was supported by Project LISBOA-01-0145-FEDER-007660 (?Microbiologia Molecular, Estrutural e Celular?) funded by FEDER funds through COMPETE2020??Programa Operacional Competitividade e Internacionaliza??o? (POCI); ?Funda??o para a Ci?ncia e a Tecnologia? (FCT) grants EXPL/BIA-MIC/2525/2013 and programme IF (IF/00124/2015) to C.P.; grant PTDC/BIA-BQM/31317/2017 to C.V.R.; the European Union?s Horizon 2020 research and innovation programme under grant agreement No. 810856 and 857203; COST Action CA15133, supported by COST (European Cooperation in Science and Technology) and PPBI?Portuguese Platform of BioImaging (PPBI-POCI-01-0145-FEDER-022122) co-funded by national funds from OE??Or?amento de Estado? and by FEDER. C.A. (SFRH/BPD/74294/2010), S.M.C. (SFRH/BD/91077/2012), C.V.R. (SFRH/BPD/94050/2013) and A.G.-C. (SFRH/BD/118866/2016) were supported by FCT contracts or fellowships. Funding Information: Funding: This work was supported by Project LISBOA-01-0145-FEDER-007660 (“Microbiologia Molecular, ?strutural e Celular”) funded by F?D?R funds through COMP?T?2020—“Programa Operacional Competitividade e Internacionalização” (POCI); “Fundação para a Ciência e a Tecno-logia” (FCT) grants ?XPL/BIA-MIC/2525/2013 and programme IF (IF/00124/2015) to C.P.; grant PTDC/BIA-BQM/31317/2017 to C.V.R.; the ?uropean Union’s Horizon 2020 research and innovation programme under grant agreement No. 810856 and 857203; COST Action CA15133, supported by COST (European Cooperation in Science and Technology) and PPBI—Portuguese Platform of Bi-oImaging (PPBI-POCI-01-0145-FEDER-022122) co-funded by national funds from OE—“Orçamento de Estado” and by FEDER. C.A. (SFRH/BPD/74294/2010), S.M.C. (SFRH/BD/91077/2012), C.V.R. (SFRH/BPD/94050/2013) and A.G.-C. (SFRH/BD/118866/2016) were supported by FCT contracts or fellowships. Publisher Copyright: © 2021 by the authors. Licensee MDPI, Basel, Switzerland.
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spelling An internal promoter drives the expression of a truncated form of ccc1 capable of protecting yeast from iron toxicityAlternative promoterGene expressionIronIron metabolismToxicityTranscriptionVacuoleYeastMicrobiologyMicrobiology (medical)VirologyFunding Information: This work was supported by Project LISBOA-01-0145-FEDER-007660 (?Microbiologia Molecular, Estrutural e Celular?) funded by FEDER funds through COMPETE2020??Programa Operacional Competitividade e Internacionaliza??o? (POCI); ?Funda??o para a Ci?ncia e a Tecnologia? (FCT) grants EXPL/BIA-MIC/2525/2013 and programme IF (IF/00124/2015) to C.P.; grant PTDC/BIA-BQM/31317/2017 to C.V.R.; the European Union?s Horizon 2020 research and innovation programme under grant agreement No. 810856 and 857203; COST Action CA15133, supported by COST (European Cooperation in Science and Technology) and PPBI?Portuguese Platform of BioImaging (PPBI-POCI-01-0145-FEDER-022122) co-funded by national funds from OE??Or?amento de Estado? and by FEDER. C.A. (SFRH/BPD/74294/2010), S.M.C. (SFRH/BD/91077/2012), C.V.R. (SFRH/BPD/94050/2013) and A.G.-C. (SFRH/BD/118866/2016) were supported by FCT contracts or fellowships. Funding Information: Funding: This work was supported by Project LISBOA-01-0145-FEDER-007660 (“Microbiologia Molecular, ?strutural e Celular”) funded by F?D?R funds through COMP?T?2020—“Programa Operacional Competitividade e Internacionalização” (POCI); “Fundação para a Ciência e a Tecno-logia” (FCT) grants ?XPL/BIA-MIC/2525/2013 and programme IF (IF/00124/2015) to C.P.; grant PTDC/BIA-BQM/31317/2017 to C.V.R.; the ?uropean Union’s Horizon 2020 research and innovation programme under grant agreement No. 810856 and 857203; COST Action CA15133, supported by COST (European Cooperation in Science and Technology) and PPBI—Portuguese Platform of Bi-oImaging (PPBI-POCI-01-0145-FEDER-022122) co-funded by national funds from OE—“Orçamento de Estado” and by FEDER. C.A. (SFRH/BPD/74294/2010), S.M.C. (SFRH/BD/91077/2012), C.V.R. (SFRH/BPD/94050/2013) and A.G.-C. (SFRH/BD/118866/2016) were supported by FCT contracts or fellowships. Publisher Copyright: © 2021 by the authors. Licensee MDPI, Basel, Switzerland.In yeast, iron storage and detoxification depend on the Ccc1 transporter that mediates iron accumulation in vacuoles. While deletion of the CCC1 gene renders cells unable to survive under iron overload conditions, the deletion of its previously identified regulators only partially affects survival, indicating that the mechanisms controlling iron storage and detoxification in yeast are still far from well understood. This work reveals that CCC1 is equipped with a complex transcriptional structure comprising several regulatory regions. One of these is located inside the coding sequence of the gene and drives the expression of a short transcript encoding an N-terminally truncated protein, designated as s-Ccc1. s-Ccc1, though less efficiently than Ccc1, is able to promote metal accumulation in the vacuole, protecting cells against iron toxicity. While the expression of the s-Ccc1 appears to be repressed in the normal genomic context, our current data clearly demonstrates that it is functional and has the capacity to play a role under iron overload conditions.Instituto de Tecnologia Química e Biológica António Xavier (ITQB)RUNAmaral, CatarinaVicente, Cristina TeixeiraCaetano, Soraia MarquesGaspar-Cordeiro, AnaYang, YangCloetens, PeterRomão, Célia V.Rodrigues-Pousada, ClaudinaPimentel, Catarina2021-12-06T23:43:23Z2021-062021-06-01T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/10362/128804eng2076-2607PURE: 35228407https://doi.org/10.3390/microorganisms9061337info:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2024-03-11T05:08:11Zoai:run.unl.pt:10362/128804Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-20T03:46:24.293961Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse
dc.title.none.fl_str_mv An internal promoter drives the expression of a truncated form of ccc1 capable of protecting yeast from iron toxicity
title An internal promoter drives the expression of a truncated form of ccc1 capable of protecting yeast from iron toxicity
spellingShingle An internal promoter drives the expression of a truncated form of ccc1 capable of protecting yeast from iron toxicity
Amaral, Catarina
Alternative promoter
Gene expression
Iron
Iron metabolism
Toxicity
Transcription
Vacuole
Yeast
Microbiology
Microbiology (medical)
Virology
title_short An internal promoter drives the expression of a truncated form of ccc1 capable of protecting yeast from iron toxicity
title_full An internal promoter drives the expression of a truncated form of ccc1 capable of protecting yeast from iron toxicity
title_fullStr An internal promoter drives the expression of a truncated form of ccc1 capable of protecting yeast from iron toxicity
title_full_unstemmed An internal promoter drives the expression of a truncated form of ccc1 capable of protecting yeast from iron toxicity
title_sort An internal promoter drives the expression of a truncated form of ccc1 capable of protecting yeast from iron toxicity
author Amaral, Catarina
author_facet Amaral, Catarina
Vicente, Cristina Teixeira
Caetano, Soraia Marques
Gaspar-Cordeiro, Ana
Yang, Yang
Cloetens, Peter
Romão, Célia V.
Rodrigues-Pousada, Claudina
Pimentel, Catarina
author_role author
author2 Vicente, Cristina Teixeira
Caetano, Soraia Marques
Gaspar-Cordeiro, Ana
Yang, Yang
Cloetens, Peter
Romão, Célia V.
Rodrigues-Pousada, Claudina
Pimentel, Catarina
author2_role author
author
author
author
author
author
author
author
dc.contributor.none.fl_str_mv Instituto de Tecnologia Química e Biológica António Xavier (ITQB)
RUN
dc.contributor.author.fl_str_mv Amaral, Catarina
Vicente, Cristina Teixeira
Caetano, Soraia Marques
Gaspar-Cordeiro, Ana
Yang, Yang
Cloetens, Peter
Romão, Célia V.
Rodrigues-Pousada, Claudina
Pimentel, Catarina
dc.subject.por.fl_str_mv Alternative promoter
Gene expression
Iron
Iron metabolism
Toxicity
Transcription
Vacuole
Yeast
Microbiology
Microbiology (medical)
Virology
topic Alternative promoter
Gene expression
Iron
Iron metabolism
Toxicity
Transcription
Vacuole
Yeast
Microbiology
Microbiology (medical)
Virology
description Funding Information: This work was supported by Project LISBOA-01-0145-FEDER-007660 (?Microbiologia Molecular, Estrutural e Celular?) funded by FEDER funds through COMPETE2020??Programa Operacional Competitividade e Internacionaliza??o? (POCI); ?Funda??o para a Ci?ncia e a Tecnologia? (FCT) grants EXPL/BIA-MIC/2525/2013 and programme IF (IF/00124/2015) to C.P.; grant PTDC/BIA-BQM/31317/2017 to C.V.R.; the European Union?s Horizon 2020 research and innovation programme under grant agreement No. 810856 and 857203; COST Action CA15133, supported by COST (European Cooperation in Science and Technology) and PPBI?Portuguese Platform of BioImaging (PPBI-POCI-01-0145-FEDER-022122) co-funded by national funds from OE??Or?amento de Estado? and by FEDER. C.A. (SFRH/BPD/74294/2010), S.M.C. (SFRH/BD/91077/2012), C.V.R. (SFRH/BPD/94050/2013) and A.G.-C. (SFRH/BD/118866/2016) were supported by FCT contracts or fellowships. Funding Information: Funding: This work was supported by Project LISBOA-01-0145-FEDER-007660 (“Microbiologia Molecular, ?strutural e Celular”) funded by F?D?R funds through COMP?T?2020—“Programa Operacional Competitividade e Internacionalização” (POCI); “Fundação para a Ciência e a Tecno-logia” (FCT) grants ?XPL/BIA-MIC/2525/2013 and programme IF (IF/00124/2015) to C.P.; grant PTDC/BIA-BQM/31317/2017 to C.V.R.; the ?uropean Union’s Horizon 2020 research and innovation programme under grant agreement No. 810856 and 857203; COST Action CA15133, supported by COST (European Cooperation in Science and Technology) and PPBI—Portuguese Platform of Bi-oImaging (PPBI-POCI-01-0145-FEDER-022122) co-funded by national funds from OE—“Orçamento de Estado” and by FEDER. C.A. (SFRH/BPD/74294/2010), S.M.C. (SFRH/BD/91077/2012), C.V.R. (SFRH/BPD/94050/2013) and A.G.-C. (SFRH/BD/118866/2016) were supported by FCT contracts or fellowships. Publisher Copyright: © 2021 by the authors. Licensee MDPI, Basel, Switzerland.
publishDate 2021
dc.date.none.fl_str_mv 2021-12-06T23:43:23Z
2021-06
2021-06-01T00:00:00Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
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url http://hdl.handle.net/10362/128804
dc.language.iso.fl_str_mv eng
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dc.relation.none.fl_str_mv 2076-2607
PURE: 35228407
https://doi.org/10.3390/microorganisms9061337
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eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
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