Establishment of ecotoxicological tests of Cadmium and Ciprofloxacin on Phaeodactylum tricornutum
Autor(a) principal: | |
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Data de Publicação: | 2023 |
Outros Autores: | , |
Tipo de documento: | Relatório |
Idioma: | eng |
Título da fonte: | Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
Texto Completo: | http://hdl.handle.net/10773/40028 |
Resumo: | Diatoms are photosynthetic organisms that are very important for the marine ecosystem and are the basis of primary production in these habitats. Knowing the importance of this group, this work aims to investigate if chemical stress affects the Epigenetic of diatoms. For that, it was verified the resistance of the microalgae Phaeodactylum tricornutum when expose to the antibiotic ciprofloxacin and to the metal cadmium. An attempt was made to verify what would be the best conditions to extract the greatest amount of DNA possible. It was verified that increasing concentrations of the antibiotic and the metal decreased the growth rate and the yield in the productivity of the cultures. It was also found that the cells when exposed to constant light show a decrease in health after 3 days under the conditions, leading to a low amount of extracted DNA. |
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Establishment of ecotoxicological tests of Cadmium and Ciprofloxacin on Phaeodactylum tricornutumBiomass productionCulture conditionToxicity testDiatoms are photosynthetic organisms that are very important for the marine ecosystem and are the basis of primary production in these habitats. Knowing the importance of this group, this work aims to investigate if chemical stress affects the Epigenetic of diatoms. For that, it was verified the resistance of the microalgae Phaeodactylum tricornutum when expose to the antibiotic ciprofloxacin and to the metal cadmium. An attempt was made to verify what would be the best conditions to extract the greatest amount of DNA possible. It was verified that increasing concentrations of the antibiotic and the metal decreased the growth rate and the yield in the productivity of the cultures. It was also found that the cells when exposed to constant light show a decrease in health after 3 days under the conditions, leading to a low amount of extracted DNA.As diatomáceas são organismos fotossintéticos muito importantes para o ecossistema marinho e são a base da produção primária nestes habitats. Sabendo da importância deste grupo, este trabalho tem como objetivo investigar se o stress químico afecta a epigenética das diatomáceas. Para isso, foi verificada a resistência da microalga Phaeodactylum tricornutum quando exposta ao antibiótico ciprofloxacina e ao metal cádmio. Procurou-se verificar quais seriam as melhores condições para extrair a maior quantidade possível de DNA. Verificou-se que concentrações crescentes do antibiótico e do metal diminuíam a taxa de crescimento e o rendimento das culturas. Verificou-se também que as células quando expostas à luz constante apresentam uma diminuição da saúde após 3 dias sob as condições, levando a uma baixa quantidade de DNA extraído.2024-01-09T15:40:10Z2023-01-01T00:00:00Z2023info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/reportapplication/pdfhttp://hdl.handle.net/10773/40028engPinhal, MafaldaSerôdio, JoãoFrankenbach, Siljainfo:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2024-02-22T12:17:33Zoai:ria.ua.pt:10773/40028Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-20T03:09:48.519178Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse |
dc.title.none.fl_str_mv |
Establishment of ecotoxicological tests of Cadmium and Ciprofloxacin on Phaeodactylum tricornutum |
title |
Establishment of ecotoxicological tests of Cadmium and Ciprofloxacin on Phaeodactylum tricornutum |
spellingShingle |
Establishment of ecotoxicological tests of Cadmium and Ciprofloxacin on Phaeodactylum tricornutum Pinhal, Mafalda Biomass production Culture condition Toxicity test |
title_short |
Establishment of ecotoxicological tests of Cadmium and Ciprofloxacin on Phaeodactylum tricornutum |
title_full |
Establishment of ecotoxicological tests of Cadmium and Ciprofloxacin on Phaeodactylum tricornutum |
title_fullStr |
Establishment of ecotoxicological tests of Cadmium and Ciprofloxacin on Phaeodactylum tricornutum |
title_full_unstemmed |
Establishment of ecotoxicological tests of Cadmium and Ciprofloxacin on Phaeodactylum tricornutum |
title_sort |
Establishment of ecotoxicological tests of Cadmium and Ciprofloxacin on Phaeodactylum tricornutum |
author |
Pinhal, Mafalda |
author_facet |
Pinhal, Mafalda Serôdio, João Frankenbach, Silja |
author_role |
author |
author2 |
Serôdio, João Frankenbach, Silja |
author2_role |
author author |
dc.contributor.author.fl_str_mv |
Pinhal, Mafalda Serôdio, João Frankenbach, Silja |
dc.subject.por.fl_str_mv |
Biomass production Culture condition Toxicity test |
topic |
Biomass production Culture condition Toxicity test |
description |
Diatoms are photosynthetic organisms that are very important for the marine ecosystem and are the basis of primary production in these habitats. Knowing the importance of this group, this work aims to investigate if chemical stress affects the Epigenetic of diatoms. For that, it was verified the resistance of the microalgae Phaeodactylum tricornutum when expose to the antibiotic ciprofloxacin and to the metal cadmium. An attempt was made to verify what would be the best conditions to extract the greatest amount of DNA possible. It was verified that increasing concentrations of the antibiotic and the metal decreased the growth rate and the yield in the productivity of the cultures. It was also found that the cells when exposed to constant light show a decrease in health after 3 days under the conditions, leading to a low amount of extracted DNA. |
publishDate |
2023 |
dc.date.none.fl_str_mv |
2023-01-01T00:00:00Z 2023 2024-01-09T15:40:10Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/report |
format |
report |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://hdl.handle.net/10773/40028 |
url |
http://hdl.handle.net/10773/40028 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.source.none.fl_str_mv |
reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação instacron:RCAAP |
instname_str |
Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
instacron_str |
RCAAP |
institution |
RCAAP |
reponame_str |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
collection |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
repository.name.fl_str_mv |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
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1799137748780580865 |