Anti-malarial resistance in Mozambique
| Autor(a) principal: | |
|---|---|
| Data de Publicação: | 2023 |
| Outros Autores: | , , , , , , , , , , , |
| Tipo de documento: | Artigo |
| Idioma: | eng |
| Título da fonte: | Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
| Texto Completo: | http://hdl.handle.net/10362/154057 |
Resumo: | © 2023. The Author(s). |
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Anti-malarial resistance in MozambiqueAbsence of Plasmodium falciparum Kelch 13 (K13) propeller domain polymorphisms associated with resistance to artemisininsAnti-malarial resistancePlasmodium falciparumpolymorphisms associated with resistanceresistance to artemisininsMozambiqueQR MicrobiologyRA0421 Public health. Hygiene. Preventive MedicineRM Therapeutics. PharmacologyPublic Health, Environmental and Occupational HealthInfectious DiseasesParasitologyMolecular BiologyGeneticsSDG 3 - Good Health and Well-being© 2023. The Author(s).BACKGROUND: Malaria remains one of the most serious public health problems in sub-Saharan Africa and Mozambique is the world's fourth largest contributor, with 4.7% of disease cases and 3.6% of total deaths due to malaria. Its control relies on the fight against the vector and treatment of confirmed cases with anti-malarial drugs. Molecular surveillance is an important tool for monitoring the spread of anti-malarial drug resistance. METHODS: A cross-sectional study recruited 450 participants with malaria infection detected by Rapid Diagnostic Tests, from three different study sites (Niassa, Manica and Maputo) between April and August 2021. Correspondent blood samples were collected on filter paper (Whatman® FTA® cards), parasite DNA extracted and pfk13 gene sequenced using Sanger method. SIFT software (Sorting Intolerant From Tolerant) was used, predict whether an amino acid substitution affects protein function. RESULTS: No pfkelch13-mediated artemisinin resistance gene mutation was detected in this study settings. However, non-synonymous mutations were detected at prevalence of 10.2%, 6% and 5% in Niassa, Manica and Maputo, respectively. Most (56.3%) of the reported non-synonymous mutations were due to substitution at the first base of the codon, 25% at the second base and 18.8% at the third base. Additionally, 50% of non-synonymous mutations showed a SIFTscore bellow cut off value of 0.05, therefore, they were predicted to be deleterious. CONCLUSION: These results do not show an emergence of artemisinin resistance cases in Mozambique. However, the increased number of novel non-synonymous mutations highlights the relevance of increasing the number of studies focused on the molecular surveillance of artemisinin resistance markers, for its early detection.Instituto de Higiene e Medicina Tropical (IHMT)Global Health and Tropical Medicine (GHTM)Vector borne diseases and pathogens (VBD)RUNda Silva, ClementeMatias, DanielaDias, BrigiteCancio, BeatrizSilva, MiguelViegas, RúbenChivale, NordinoLuis, SoniaSalvador, CrizolgoDuarte, DeniseArnaldo, PauloEnosse, SoniaNogueira, Fatima2023-06-16T22:19:47Z2023-05-192023-05-19T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article6application/pdfhttp://hdl.handle.net/10362/154057eng1475-2875PURE: 61131706https://doi.org/10.1186/s12936-023-04589-0info:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2024-03-11T05:36:33Zoai:run.unl.pt:10362/154057Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-20T03:55:29.441084Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse |
| dc.title.none.fl_str_mv |
Anti-malarial resistance in Mozambique Absence of Plasmodium falciparum Kelch 13 (K13) propeller domain polymorphisms associated with resistance to artemisinins |
| title |
Anti-malarial resistance in Mozambique |
| spellingShingle |
Anti-malarial resistance in Mozambique da Silva, Clemente Anti-malarial resistance Plasmodium falciparum polymorphisms associated with resistance resistance to artemisinins Mozambique QR Microbiology RA0421 Public health. Hygiene. Preventive Medicine RM Therapeutics. Pharmacology Public Health, Environmental and Occupational Health Infectious Diseases Parasitology Molecular Biology Genetics SDG 3 - Good Health and Well-being |
| title_short |
Anti-malarial resistance in Mozambique |
| title_full |
Anti-malarial resistance in Mozambique |
| title_fullStr |
Anti-malarial resistance in Mozambique |
| title_full_unstemmed |
Anti-malarial resistance in Mozambique |
| title_sort |
Anti-malarial resistance in Mozambique |
| author |
da Silva, Clemente |
| author_facet |
da Silva, Clemente Matias, Daniela Dias, Brigite Cancio, Beatriz Silva, Miguel Viegas, Rúben Chivale, Nordino Luis, Sonia Salvador, Crizolgo Duarte, Denise Arnaldo, Paulo Enosse, Sonia Nogueira, Fatima |
| author_role |
author |
| author2 |
Matias, Daniela Dias, Brigite Cancio, Beatriz Silva, Miguel Viegas, Rúben Chivale, Nordino Luis, Sonia Salvador, Crizolgo Duarte, Denise Arnaldo, Paulo Enosse, Sonia Nogueira, Fatima |
| author2_role |
author author author author author author author author author author author author |
| dc.contributor.none.fl_str_mv |
Instituto de Higiene e Medicina Tropical (IHMT) Global Health and Tropical Medicine (GHTM) Vector borne diseases and pathogens (VBD) RUN |
| dc.contributor.author.fl_str_mv |
da Silva, Clemente Matias, Daniela Dias, Brigite Cancio, Beatriz Silva, Miguel Viegas, Rúben Chivale, Nordino Luis, Sonia Salvador, Crizolgo Duarte, Denise Arnaldo, Paulo Enosse, Sonia Nogueira, Fatima |
| dc.subject.por.fl_str_mv |
Anti-malarial resistance Plasmodium falciparum polymorphisms associated with resistance resistance to artemisinins Mozambique QR Microbiology RA0421 Public health. Hygiene. Preventive Medicine RM Therapeutics. Pharmacology Public Health, Environmental and Occupational Health Infectious Diseases Parasitology Molecular Biology Genetics SDG 3 - Good Health and Well-being |
| topic |
Anti-malarial resistance Plasmodium falciparum polymorphisms associated with resistance resistance to artemisinins Mozambique QR Microbiology RA0421 Public health. Hygiene. Preventive Medicine RM Therapeutics. Pharmacology Public Health, Environmental and Occupational Health Infectious Diseases Parasitology Molecular Biology Genetics SDG 3 - Good Health and Well-being |
| description |
© 2023. The Author(s). |
| publishDate |
2023 |
| dc.date.none.fl_str_mv |
2023-06-16T22:19:47Z 2023-05-19 2023-05-19T00:00:00Z |
| dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
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info:eu-repo/semantics/article |
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article |
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publishedVersion |
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http://hdl.handle.net/10362/154057 |
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http://hdl.handle.net/10362/154057 |
| dc.language.iso.fl_str_mv |
eng |
| language |
eng |
| dc.relation.none.fl_str_mv |
1475-2875 PURE: 61131706 https://doi.org/10.1186/s12936-023-04589-0 |
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info:eu-repo/semantics/openAccess |
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openAccess |
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6 application/pdf |
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reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação instacron:RCAAP |
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Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
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RCAAP |
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RCAAP |
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Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
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Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
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Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
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