Molecular Epidemiology and Risk Factors of Carbapenemase-Producing Enterobacteriaceae Isolates in Portuguese Hospitals: Results From European Survey on Carbapenemase-Producing Enterobacteriaceae (EuSCAPE)

Detalhes bibliográficos
Autor(a) principal: Manageiro, Vera
Data de Publicação: 2018
Outros Autores: Romão, Raquel, Moura, Inês Barata, Sampaio, Daniel A., Vieira, Luís, Ferreira, Eugénia, Caniça, Manuela
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
Texto Completo: http://hdl.handle.net/10400.18/6330
Resumo: Free PMC Article: https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6277554/
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spelling Molecular Epidemiology and Risk Factors of Carbapenemase-Producing Enterobacteriaceae Isolates in Portuguese Hospitals: Results From European Survey on Carbapenemase-Producing Enterobacteriaceae (EuSCAPE)Escherichia coliEuSCAPEKPC-21Klebsiella pneumoniaePortugalCarbapenemase-producing EnterobacteriaceaeResistência aos AntimicrobianosFree PMC Article: https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6277554/In Portugal, the epidemiological stage for the spread of carbapenemase-producing Enterobacteriaceae (CPE) increased from sporadic isolates or single hospital clones (2010-2013), to hospital outbreaks, later. Here we report data from a 6-month study performed under the European Survey on Carbapenemase-Producing Enterobacteriaceae (EuSCAPE). During the study period, 67 isolates (61 Klebsiella pneumoniae and 6 Escherichia coli) non-susceptible to carbapenems were identified in participant hospital laboratories. We detected 37 blaKPC-type (including one new variant: blaKPC-21), 1 blaGES-5, and 1 blaGES-6 plus blaKPC-3, alone or in combination with other bla genes. Bioinformatics analysis of the KPC-21-producing E. coli identified the new variant blaKPC-21 in a 12,748 bp length plasmid. The blaKPC-21 gene was harbored on a non-Tn4401 element, presenting upstream a partial ISKpn6 (ΔISKpn6/ΔtraN) with the related left IR (IRL) and downstream a truncated Tn3 transposon. PFGE and MLST analysis showed an important diversity, as isolates belonged to distinct PFGE and STs profiles. In this study, we highlighted the presence of the high-risk clone E. coli sequence-type (ST) 131 clade C/H30. This worldwide disseminated E. coli lineage was already detected in Portugal among other antibiotic resistance reservoirs. This study highlights the intra- and inter-hospital spread and possible intercontinental circulation of CPE isolates.VM was supported by Fundação para a Ciência e a Tecnologia (FCT) fellowship (Grant SFRH/BPD/ 77486/2011), financed by the European Social Funds (COMPETE-FEDER) and national funds of the Portuguese Ministry of Education and Science (POPH-QREN). The authors thank FCT for Project grant UID/MULTI/00211/2013.Frontiers MediaRepositório Científico do Instituto Nacional de SaúdeManageiro, VeraRomão, RaquelMoura, Inês BarataSampaio, Daniel A.Vieira, LuísFerreira, EugéniaCaniça, Manuela2019-03-27T13:14:54Z2018-11-272018-11-27T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/10400.18/6330engFront Microbiol. 2018 Nov 27;9:2834. doi: 10.3389/fmicb.2018.02834. eCollection 2018.10.3389/fmicb.2018.02834info:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2023-07-20T15:41:21Zoai:repositorio.insa.pt:10400.18/6330Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-19T18:40:58.799589Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse
dc.title.none.fl_str_mv Molecular Epidemiology and Risk Factors of Carbapenemase-Producing Enterobacteriaceae Isolates in Portuguese Hospitals: Results From European Survey on Carbapenemase-Producing Enterobacteriaceae (EuSCAPE)
title Molecular Epidemiology and Risk Factors of Carbapenemase-Producing Enterobacteriaceae Isolates in Portuguese Hospitals: Results From European Survey on Carbapenemase-Producing Enterobacteriaceae (EuSCAPE)
spellingShingle Molecular Epidemiology and Risk Factors of Carbapenemase-Producing Enterobacteriaceae Isolates in Portuguese Hospitals: Results From European Survey on Carbapenemase-Producing Enterobacteriaceae (EuSCAPE)
Manageiro, Vera
Escherichia coli
EuSCAPE
KPC-21
Klebsiella pneumoniae
Portugal
Carbapenemase-producing Enterobacteriaceae
Resistência aos Antimicrobianos
title_short Molecular Epidemiology and Risk Factors of Carbapenemase-Producing Enterobacteriaceae Isolates in Portuguese Hospitals: Results From European Survey on Carbapenemase-Producing Enterobacteriaceae (EuSCAPE)
title_full Molecular Epidemiology and Risk Factors of Carbapenemase-Producing Enterobacteriaceae Isolates in Portuguese Hospitals: Results From European Survey on Carbapenemase-Producing Enterobacteriaceae (EuSCAPE)
title_fullStr Molecular Epidemiology and Risk Factors of Carbapenemase-Producing Enterobacteriaceae Isolates in Portuguese Hospitals: Results From European Survey on Carbapenemase-Producing Enterobacteriaceae (EuSCAPE)
title_full_unstemmed Molecular Epidemiology and Risk Factors of Carbapenemase-Producing Enterobacteriaceae Isolates in Portuguese Hospitals: Results From European Survey on Carbapenemase-Producing Enterobacteriaceae (EuSCAPE)
title_sort Molecular Epidemiology and Risk Factors of Carbapenemase-Producing Enterobacteriaceae Isolates in Portuguese Hospitals: Results From European Survey on Carbapenemase-Producing Enterobacteriaceae (EuSCAPE)
author Manageiro, Vera
author_facet Manageiro, Vera
Romão, Raquel
Moura, Inês Barata
Sampaio, Daniel A.
Vieira, Luís
Ferreira, Eugénia
Caniça, Manuela
author_role author
author2 Romão, Raquel
Moura, Inês Barata
Sampaio, Daniel A.
Vieira, Luís
Ferreira, Eugénia
Caniça, Manuela
author2_role author
author
author
author
author
author
dc.contributor.none.fl_str_mv Repositório Científico do Instituto Nacional de Saúde
dc.contributor.author.fl_str_mv Manageiro, Vera
Romão, Raquel
Moura, Inês Barata
Sampaio, Daniel A.
Vieira, Luís
Ferreira, Eugénia
Caniça, Manuela
dc.subject.por.fl_str_mv Escherichia coli
EuSCAPE
KPC-21
Klebsiella pneumoniae
Portugal
Carbapenemase-producing Enterobacteriaceae
Resistência aos Antimicrobianos
topic Escherichia coli
EuSCAPE
KPC-21
Klebsiella pneumoniae
Portugal
Carbapenemase-producing Enterobacteriaceae
Resistência aos Antimicrobianos
description Free PMC Article: https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6277554/
publishDate 2018
dc.date.none.fl_str_mv 2018-11-27
2018-11-27T00:00:00Z
2019-03-27T13:14:54Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://hdl.handle.net/10400.18/6330
url http://hdl.handle.net/10400.18/6330
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv Front Microbiol. 2018 Nov 27;9:2834. doi: 10.3389/fmicb.2018.02834. eCollection 2018.
10.3389/fmicb.2018.02834
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Frontiers Media
publisher.none.fl_str_mv Frontiers Media
dc.source.none.fl_str_mv reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron:RCAAP
instname_str Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron_str RCAAP
institution RCAAP
reponame_str Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
collection Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
repository.name.fl_str_mv Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
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