Molecular epidemiology and risk factors of carbapenemase-producing Enterobacteriaceae isolates in Portuguese hospitals
Autor(a) principal: | |
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Data de Publicação: | 2018 |
Outros Autores: | , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
Texto Completo: | https://doi.org/10.3389/fmicb.2018.02834 |
Resumo: | In Portugal, the epidemiological stage for the spread of carbapenemase-producing Enterobacteriaceae (CPE) increased from sporadic isolates or single hospital clones (2010–2013), to hospital outbreaks, later. Here we report data from a 6-month study performed under the European Survey on Carbapenemase-Producing Enterobacteriaceae (EuSCAPE). During the study period, 67 isolates (61 Klebsiella pneumoniae and 6 Escherichia coli) non-susceptible to carbapenems were identified in participant hospital laboratories. We detected 37 blaKPC–type (including one new variant: blaKPC–21), 1 blaGES–5, and 1 blaGES–6 plus blaKPC–3, alone or in combination with other bla genes. Bioinformatics analysis of the KPC-21-producing E. coli identified the new variant blaKPC–21 in a 12,748 bp length plasmid. The blaKPC–21 gene was harbored on a non-Tn4401 element, presenting upstream a partial ISKpn6 (ΔISKpn6/ΔtraN) with the related left IR (IRL) and downstream a truncated Tn3 transposon. PFGE and MLST analysis showed an important diversity, as isolates belonged to distinct PFGE and STs profiles. In this study, we highlighted the presence of the high-risk clone E. coli sequence-type (ST) 131 clade C/H30. This worldwide disseminated E. coli lineage was already detected in Portugal among other antibiotic resistance reservoirs. This study highlights the intra- and inter-hospital spread and possible intercontinental circulation of CPE isolates. |
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Molecular epidemiology and risk factors of carbapenemase-producing Enterobacteriaceae isolates in Portuguese hospitalsResults From European survey on carbapenemase-producing Enterobacteriaceae (EuSCAPE)Carbapenemase-producing EnterobacteriaceaeEscherichia coliEuSCAPEKlebsiella pneumoniaeKPC-21PortugalMicrobiologyMicrobiology (medical)In Portugal, the epidemiological stage for the spread of carbapenemase-producing Enterobacteriaceae (CPE) increased from sporadic isolates or single hospital clones (2010–2013), to hospital outbreaks, later. Here we report data from a 6-month study performed under the European Survey on Carbapenemase-Producing Enterobacteriaceae (EuSCAPE). During the study period, 67 isolates (61 Klebsiella pneumoniae and 6 Escherichia coli) non-susceptible to carbapenems were identified in participant hospital laboratories. We detected 37 blaKPC–type (including one new variant: blaKPC–21), 1 blaGES–5, and 1 blaGES–6 plus blaKPC–3, alone or in combination with other bla genes. Bioinformatics analysis of the KPC-21-producing E. coli identified the new variant blaKPC–21 in a 12,748 bp length plasmid. The blaKPC–21 gene was harbored on a non-Tn4401 element, presenting upstream a partial ISKpn6 (ΔISKpn6/ΔtraN) with the related left IR (IRL) and downstream a truncated Tn3 transposon. PFGE and MLST analysis showed an important diversity, as isolates belonged to distinct PFGE and STs profiles. In this study, we highlighted the presence of the high-risk clone E. coli sequence-type (ST) 131 clade C/H30. This worldwide disseminated E. coli lineage was already detected in Portugal among other antibiotic resistance reservoirs. This study highlights the intra- and inter-hospital spread and possible intercontinental circulation of CPE isolates.NOVA Medical School|Faculdade de Ciências Médicas (NMS|FCM)Centre for Toxicogenomics and Human Health (ToxOmics)RUNManageiro, VeraRomão, RaquelMoura, Inês BarataSampaio, Daniel A.Vieira, LuísFerreira, EugéniaCaniça, Manuela2019-03-28T23:29:52Z2018-11-272018-11-27T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttps://doi.org/10.3389/fmicb.2018.02834eng1664-302XPURE: 12430040http://www.scopus.com/inward/record.url?scp=85057744733&partnerID=8YFLogxKhttps://doi.org/10.3389/fmicb.2018.02834info:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2024-03-11T04:30:51Zoai:run.unl.pt:10362/64925Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-20T03:34:13.502770Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse |
dc.title.none.fl_str_mv |
Molecular epidemiology and risk factors of carbapenemase-producing Enterobacteriaceae isolates in Portuguese hospitals Results From European survey on carbapenemase-producing Enterobacteriaceae (EuSCAPE) |
title |
Molecular epidemiology and risk factors of carbapenemase-producing Enterobacteriaceae isolates in Portuguese hospitals |
spellingShingle |
Molecular epidemiology and risk factors of carbapenemase-producing Enterobacteriaceae isolates in Portuguese hospitals Manageiro, Vera Carbapenemase-producing Enterobacteriaceae Escherichia coli EuSCAPE Klebsiella pneumoniae KPC-21 Portugal Microbiology Microbiology (medical) |
title_short |
Molecular epidemiology and risk factors of carbapenemase-producing Enterobacteriaceae isolates in Portuguese hospitals |
title_full |
Molecular epidemiology and risk factors of carbapenemase-producing Enterobacteriaceae isolates in Portuguese hospitals |
title_fullStr |
Molecular epidemiology and risk factors of carbapenemase-producing Enterobacteriaceae isolates in Portuguese hospitals |
title_full_unstemmed |
Molecular epidemiology and risk factors of carbapenemase-producing Enterobacteriaceae isolates in Portuguese hospitals |
title_sort |
Molecular epidemiology and risk factors of carbapenemase-producing Enterobacteriaceae isolates in Portuguese hospitals |
author |
Manageiro, Vera |
author_facet |
Manageiro, Vera Romão, Raquel Moura, Inês Barata Sampaio, Daniel A. Vieira, Luís Ferreira, Eugénia Caniça, Manuela |
author_role |
author |
author2 |
Romão, Raquel Moura, Inês Barata Sampaio, Daniel A. Vieira, Luís Ferreira, Eugénia Caniça, Manuela |
author2_role |
author author author author author author |
dc.contributor.none.fl_str_mv |
NOVA Medical School|Faculdade de Ciências Médicas (NMS|FCM) Centre for Toxicogenomics and Human Health (ToxOmics) RUN |
dc.contributor.author.fl_str_mv |
Manageiro, Vera Romão, Raquel Moura, Inês Barata Sampaio, Daniel A. Vieira, Luís Ferreira, Eugénia Caniça, Manuela |
dc.subject.por.fl_str_mv |
Carbapenemase-producing Enterobacteriaceae Escherichia coli EuSCAPE Klebsiella pneumoniae KPC-21 Portugal Microbiology Microbiology (medical) |
topic |
Carbapenemase-producing Enterobacteriaceae Escherichia coli EuSCAPE Klebsiella pneumoniae KPC-21 Portugal Microbiology Microbiology (medical) |
description |
In Portugal, the epidemiological stage for the spread of carbapenemase-producing Enterobacteriaceae (CPE) increased from sporadic isolates or single hospital clones (2010–2013), to hospital outbreaks, later. Here we report data from a 6-month study performed under the European Survey on Carbapenemase-Producing Enterobacteriaceae (EuSCAPE). During the study period, 67 isolates (61 Klebsiella pneumoniae and 6 Escherichia coli) non-susceptible to carbapenems were identified in participant hospital laboratories. We detected 37 blaKPC–type (including one new variant: blaKPC–21), 1 blaGES–5, and 1 blaGES–6 plus blaKPC–3, alone or in combination with other bla genes. Bioinformatics analysis of the KPC-21-producing E. coli identified the new variant blaKPC–21 in a 12,748 bp length plasmid. The blaKPC–21 gene was harbored on a non-Tn4401 element, presenting upstream a partial ISKpn6 (ΔISKpn6/ΔtraN) with the related left IR (IRL) and downstream a truncated Tn3 transposon. PFGE and MLST analysis showed an important diversity, as isolates belonged to distinct PFGE and STs profiles. In this study, we highlighted the presence of the high-risk clone E. coli sequence-type (ST) 131 clade C/H30. This worldwide disseminated E. coli lineage was already detected in Portugal among other antibiotic resistance reservoirs. This study highlights the intra- and inter-hospital spread and possible intercontinental circulation of CPE isolates. |
publishDate |
2018 |
dc.date.none.fl_str_mv |
2018-11-27 2018-11-27T00:00:00Z 2019-03-28T23:29:52Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
https://doi.org/10.3389/fmicb.2018.02834 |
url |
https://doi.org/10.3389/fmicb.2018.02834 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
1664-302X PURE: 12430040 http://www.scopus.com/inward/record.url?scp=85057744733&partnerID=8YFLogxK https://doi.org/10.3389/fmicb.2018.02834 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
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application/pdf |
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