A new cyanobacterial species with a protective effect on lettuce grown under salinity stress: envisaging sustainable agriculture practices
Autor(a) principal: | |
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Data de Publicação: | 2022 |
Outros Autores: | , , , , , , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
Texto Completo: | http://hdl.handle.net/10198/25087 |
Resumo: | In this work, a new terrestrial cyanobacterial species, Oculatella lusitanica LEGE 161147, was isolated and characterized using a polyphasic approach. Morphologically, O. lusitanica shares characteristics with different Oculatella species (mainly with O. crustae-formantes), lacking distinctive features. However, the phylogeny based on the 16S rRNA gene sequence and the 16S-23S ITS secondary structures support the establishment of this isolate as a new species. O. lusitanica is placed within a clade mainly composed by other Oculatella terrestrial strains; however, it forms a separate lineage. In addition, our species differs from the other Oculatella described so far by lacking the V2 helix within the ITS region. Since cyanobacteria are known to release compounds that promote plant growth and/or increase their tolerance to stresses, the effect of this newly described cyanobacterial species on Lactuca sativa (lettuce) plants development and salinity stress resistance was evaluated. Our results showed that, although the cyanobacterium had no impact on plant growth under the conditions tested, it was able to mitigate the deleterious salinity stress effects on plant size, root and aerial part fresh weight, by eliciting the non-enzymatic antioxidant response system (proline, H2O2 and reduced glutathione). In addition, the microorganism was able to induce a priming effect on lettuce plants by stimulating defensive mechanisms under non-stress conditions, and enhances the activity of nitrogen metabolism-related enzymes glutamate dehydrogenase, glutamine synthetase and nitrate reductase. These results indicate that this native terrestrial cyanobacterial species could be employed as a tool in sustainable agricultural practices. |
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A new cyanobacterial species with a protective effect on lettuce grown under salinity stress: envisaging sustainable agriculture practicesBiostimulantsCyanobacteriaLettuceOculatella lusitanicaPhylogenySalinity stressIn this work, a new terrestrial cyanobacterial species, Oculatella lusitanica LEGE 161147, was isolated and characterized using a polyphasic approach. Morphologically, O. lusitanica shares characteristics with different Oculatella species (mainly with O. crustae-formantes), lacking distinctive features. However, the phylogeny based on the 16S rRNA gene sequence and the 16S-23S ITS secondary structures support the establishment of this isolate as a new species. O. lusitanica is placed within a clade mainly composed by other Oculatella terrestrial strains; however, it forms a separate lineage. In addition, our species differs from the other Oculatella described so far by lacking the V2 helix within the ITS region. Since cyanobacteria are known to release compounds that promote plant growth and/or increase their tolerance to stresses, the effect of this newly described cyanobacterial species on Lactuca sativa (lettuce) plants development and salinity stress resistance was evaluated. Our results showed that, although the cyanobacterium had no impact on plant growth under the conditions tested, it was able to mitigate the deleterious salinity stress effects on plant size, root and aerial part fresh weight, by eliciting the non-enzymatic antioxidant response system (proline, H2O2 and reduced glutathione). In addition, the microorganism was able to induce a priming effect on lettuce plants by stimulating defensive mechanisms under non-stress conditions, and enhances the activity of nitrogen metabolism-related enzymes glutamate dehydrogenase, glutamine synthetase and nitrate reductase. These results indicate that this native terrestrial cyanobacterial species could be employed as a tool in sustainable agricultural practices.This work was funded by National Funds through FCT—Fundação para a Ciência e a Tecnologia, I.P., under the projects PCIF/RPG/0077/2017, UIDB/04293/2020, UIDP/04293/2020, UIDB/05748/2020 and UIDP/05748/2020. This work was also funded by the FCT grant SFRH/BPD/115571/2016 (to AB) and LTAUSA 18008 (to JK)Biblioteca Digital do IPBBrito, ÂngelaRocha, MarianaKaštovský, JanVieira, JorgeVieira, Cristina P.Ramos, VitorCorreia, ManuelSantos, MarinaMota, RitaRoque, JéssicaPissarra, JoãoMelo, PaulaTamagnini, Paula2022-02-21T15:52:13Z20222022-01-01T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/10198/25087engBrito, Ângela; Rocha, Mariana; Kaštovský, Jan; Vieira, Jorge; Vieira, Cristina P.; Ramos, Vitor; Correia, Manuel; Santos, Marina; Mota, Rita; Roque, Jéssica; Pissarra, João; Melo, Paula; Tamagnini, Paula (2022). A new cyanobacterial species with a protective effect on lettuce grown under salinity stress: envisaging sustainable agriculture practices. Journal of Applied Phycology. ISSN 0921-8971. p. 1-140921-897110.1007/s10811-022-02692-4info:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2023-11-21T10:56:05Zoai:bibliotecadigital.ipb.pt:10198/25087Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-19T23:15:48.086017Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse |
dc.title.none.fl_str_mv |
A new cyanobacterial species with a protective effect on lettuce grown under salinity stress: envisaging sustainable agriculture practices |
title |
A new cyanobacterial species with a protective effect on lettuce grown under salinity stress: envisaging sustainable agriculture practices |
spellingShingle |
A new cyanobacterial species with a protective effect on lettuce grown under salinity stress: envisaging sustainable agriculture practices Brito, Ângela Biostimulants Cyanobacteria Lettuce Oculatella lusitanica Phylogeny Salinity stress |
title_short |
A new cyanobacterial species with a protective effect on lettuce grown under salinity stress: envisaging sustainable agriculture practices |
title_full |
A new cyanobacterial species with a protective effect on lettuce grown under salinity stress: envisaging sustainable agriculture practices |
title_fullStr |
A new cyanobacterial species with a protective effect on lettuce grown under salinity stress: envisaging sustainable agriculture practices |
title_full_unstemmed |
A new cyanobacterial species with a protective effect on lettuce grown under salinity stress: envisaging sustainable agriculture practices |
title_sort |
A new cyanobacterial species with a protective effect on lettuce grown under salinity stress: envisaging sustainable agriculture practices |
author |
Brito, Ângela |
author_facet |
Brito, Ângela Rocha, Mariana Kaštovský, Jan Vieira, Jorge Vieira, Cristina P. Ramos, Vitor Correia, Manuel Santos, Marina Mota, Rita Roque, Jéssica Pissarra, João Melo, Paula Tamagnini, Paula |
author_role |
author |
author2 |
Rocha, Mariana Kaštovský, Jan Vieira, Jorge Vieira, Cristina P. Ramos, Vitor Correia, Manuel Santos, Marina Mota, Rita Roque, Jéssica Pissarra, João Melo, Paula Tamagnini, Paula |
author2_role |
author author author author author author author author author author author author |
dc.contributor.none.fl_str_mv |
Biblioteca Digital do IPB |
dc.contributor.author.fl_str_mv |
Brito, Ângela Rocha, Mariana Kaštovský, Jan Vieira, Jorge Vieira, Cristina P. Ramos, Vitor Correia, Manuel Santos, Marina Mota, Rita Roque, Jéssica Pissarra, João Melo, Paula Tamagnini, Paula |
dc.subject.por.fl_str_mv |
Biostimulants Cyanobacteria Lettuce Oculatella lusitanica Phylogeny Salinity stress |
topic |
Biostimulants Cyanobacteria Lettuce Oculatella lusitanica Phylogeny Salinity stress |
description |
In this work, a new terrestrial cyanobacterial species, Oculatella lusitanica LEGE 161147, was isolated and characterized using a polyphasic approach. Morphologically, O. lusitanica shares characteristics with different Oculatella species (mainly with O. crustae-formantes), lacking distinctive features. However, the phylogeny based on the 16S rRNA gene sequence and the 16S-23S ITS secondary structures support the establishment of this isolate as a new species. O. lusitanica is placed within a clade mainly composed by other Oculatella terrestrial strains; however, it forms a separate lineage. In addition, our species differs from the other Oculatella described so far by lacking the V2 helix within the ITS region. Since cyanobacteria are known to release compounds that promote plant growth and/or increase their tolerance to stresses, the effect of this newly described cyanobacterial species on Lactuca sativa (lettuce) plants development and salinity stress resistance was evaluated. Our results showed that, although the cyanobacterium had no impact on plant growth under the conditions tested, it was able to mitigate the deleterious salinity stress effects on plant size, root and aerial part fresh weight, by eliciting the non-enzymatic antioxidant response system (proline, H2O2 and reduced glutathione). In addition, the microorganism was able to induce a priming effect on lettuce plants by stimulating defensive mechanisms under non-stress conditions, and enhances the activity of nitrogen metabolism-related enzymes glutamate dehydrogenase, glutamine synthetase and nitrate reductase. These results indicate that this native terrestrial cyanobacterial species could be employed as a tool in sustainable agricultural practices. |
publishDate |
2022 |
dc.date.none.fl_str_mv |
2022-02-21T15:52:13Z 2022 2022-01-01T00:00:00Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://hdl.handle.net/10198/25087 |
url |
http://hdl.handle.net/10198/25087 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Brito, Ângela; Rocha, Mariana; Kaštovský, Jan; Vieira, Jorge; Vieira, Cristina P.; Ramos, Vitor; Correia, Manuel; Santos, Marina; Mota, Rita; Roque, Jéssica; Pissarra, João; Melo, Paula; Tamagnini, Paula (2022). A new cyanobacterial species with a protective effect on lettuce grown under salinity stress: envisaging sustainable agriculture practices. Journal of Applied Phycology. ISSN 0921-8971. p. 1-14 0921-8971 10.1007/s10811-022-02692-4 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
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openAccess |
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application/pdf |
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Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
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Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
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