A new cyanobacterial species with a protective effect on lettuce grown under salinity stress: envisaging sustainable agriculture practices

Detalhes bibliográficos
Autor(a) principal: Brito, Ângela
Data de Publicação: 2022
Outros Autores: Rocha, Mariana, Kaštovský, Jan, Vieira, Jorge, Vieira, Cristina P., Ramos, Vitor, Correia, Manuel, Santos, Marina, Mota, Rita, Roque, Jéssica, Pissarra, João, Melo, Paula, Tamagnini, Paula
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
Texto Completo: http://hdl.handle.net/10198/25087
Resumo: In this work, a new terrestrial cyanobacterial species, Oculatella lusitanica LEGE 161147, was isolated and characterized using a polyphasic approach. Morphologically, O. lusitanica shares characteristics with different Oculatella species (mainly with O. crustae-formantes), lacking distinctive features. However, the phylogeny based on the 16S rRNA gene sequence and the 16S-23S ITS secondary structures support the establishment of this isolate as a new species. O. lusitanica is placed within a clade mainly composed by other Oculatella terrestrial strains; however, it forms a separate lineage. In addition, our species differs from the other Oculatella described so far by lacking the V2 helix within the ITS region. Since cyanobacteria are known to release compounds that promote plant growth and/or increase their tolerance to stresses, the effect of this newly described cyanobacterial species on Lactuca sativa (lettuce) plants development and salinity stress resistance was evaluated. Our results showed that, although the cyanobacterium had no impact on plant growth under the conditions tested, it was able to mitigate the deleterious salinity stress effects on plant size, root and aerial part fresh weight, by eliciting the non-enzymatic antioxidant response system (proline, H2O2 and reduced glutathione). In addition, the microorganism was able to induce a priming effect on lettuce plants by stimulating defensive mechanisms under non-stress conditions, and enhances the activity of nitrogen metabolism-related enzymes glutamate dehydrogenase, glutamine synthetase and nitrate reductase. These results indicate that this native terrestrial cyanobacterial species could be employed as a tool in sustainable agricultural practices.
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spelling A new cyanobacterial species with a protective effect on lettuce grown under salinity stress: envisaging sustainable agriculture practicesBiostimulantsCyanobacteriaLettuceOculatella lusitanicaPhylogenySalinity stressIn this work, a new terrestrial cyanobacterial species, Oculatella lusitanica LEGE 161147, was isolated and characterized using a polyphasic approach. Morphologically, O. lusitanica shares characteristics with different Oculatella species (mainly with O. crustae-formantes), lacking distinctive features. However, the phylogeny based on the 16S rRNA gene sequence and the 16S-23S ITS secondary structures support the establishment of this isolate as a new species. O. lusitanica is placed within a clade mainly composed by other Oculatella terrestrial strains; however, it forms a separate lineage. In addition, our species differs from the other Oculatella described so far by lacking the V2 helix within the ITS region. Since cyanobacteria are known to release compounds that promote plant growth and/or increase their tolerance to stresses, the effect of this newly described cyanobacterial species on Lactuca sativa (lettuce) plants development and salinity stress resistance was evaluated. Our results showed that, although the cyanobacterium had no impact on plant growth under the conditions tested, it was able to mitigate the deleterious salinity stress effects on plant size, root and aerial part fresh weight, by eliciting the non-enzymatic antioxidant response system (proline, H2O2 and reduced glutathione). In addition, the microorganism was able to induce a priming effect on lettuce plants by stimulating defensive mechanisms under non-stress conditions, and enhances the activity of nitrogen metabolism-related enzymes glutamate dehydrogenase, glutamine synthetase and nitrate reductase. These results indicate that this native terrestrial cyanobacterial species could be employed as a tool in sustainable agricultural practices.This work was funded by National Funds through FCT—Fundação para a Ciência e a Tecnologia, I.P., under the projects PCIF/RPG/0077/2017, UIDB/04293/2020, UIDP/04293/2020, UIDB/05748/2020 and UIDP/05748/2020. This work was also funded by the FCT grant SFRH/BPD/115571/2016 (to AB) and LTAUSA 18008 (to JK)Biblioteca Digital do IPBBrito, ÂngelaRocha, MarianaKaštovský, JanVieira, JorgeVieira, Cristina P.Ramos, VitorCorreia, ManuelSantos, MarinaMota, RitaRoque, JéssicaPissarra, JoãoMelo, PaulaTamagnini, Paula2022-02-21T15:52:13Z20222022-01-01T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/10198/25087engBrito, Ângela; Rocha, Mariana; Kaštovský, Jan; Vieira, Jorge; Vieira, Cristina P.; Ramos, Vitor; Correia, Manuel; Santos, Marina; Mota, Rita; Roque, Jéssica; Pissarra, João; Melo, Paula; Tamagnini, Paula (2022). A new cyanobacterial species with a protective effect on lettuce grown under salinity stress: envisaging sustainable agriculture practices. Journal of Applied Phycology. ISSN 0921-8971. p. 1-140921-897110.1007/s10811-022-02692-4info:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2023-11-21T10:56:05Zoai:bibliotecadigital.ipb.pt:10198/25087Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-19T23:15:48.086017Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse
dc.title.none.fl_str_mv A new cyanobacterial species with a protective effect on lettuce grown under salinity stress: envisaging sustainable agriculture practices
title A new cyanobacterial species with a protective effect on lettuce grown under salinity stress: envisaging sustainable agriculture practices
spellingShingle A new cyanobacterial species with a protective effect on lettuce grown under salinity stress: envisaging sustainable agriculture practices
Brito, Ângela
Biostimulants
Cyanobacteria
Lettuce
Oculatella lusitanica
Phylogeny
Salinity stress
title_short A new cyanobacterial species with a protective effect on lettuce grown under salinity stress: envisaging sustainable agriculture practices
title_full A new cyanobacterial species with a protective effect on lettuce grown under salinity stress: envisaging sustainable agriculture practices
title_fullStr A new cyanobacterial species with a protective effect on lettuce grown under salinity stress: envisaging sustainable agriculture practices
title_full_unstemmed A new cyanobacterial species with a protective effect on lettuce grown under salinity stress: envisaging sustainable agriculture practices
title_sort A new cyanobacterial species with a protective effect on lettuce grown under salinity stress: envisaging sustainable agriculture practices
author Brito, Ângela
author_facet Brito, Ângela
Rocha, Mariana
Kaštovský, Jan
Vieira, Jorge
Vieira, Cristina P.
Ramos, Vitor
Correia, Manuel
Santos, Marina
Mota, Rita
Roque, Jéssica
Pissarra, João
Melo, Paula
Tamagnini, Paula
author_role author
author2 Rocha, Mariana
Kaštovský, Jan
Vieira, Jorge
Vieira, Cristina P.
Ramos, Vitor
Correia, Manuel
Santos, Marina
Mota, Rita
Roque, Jéssica
Pissarra, João
Melo, Paula
Tamagnini, Paula
author2_role author
author
author
author
author
author
author
author
author
author
author
author
dc.contributor.none.fl_str_mv Biblioteca Digital do IPB
dc.contributor.author.fl_str_mv Brito, Ângela
Rocha, Mariana
Kaštovský, Jan
Vieira, Jorge
Vieira, Cristina P.
Ramos, Vitor
Correia, Manuel
Santos, Marina
Mota, Rita
Roque, Jéssica
Pissarra, João
Melo, Paula
Tamagnini, Paula
dc.subject.por.fl_str_mv Biostimulants
Cyanobacteria
Lettuce
Oculatella lusitanica
Phylogeny
Salinity stress
topic Biostimulants
Cyanobacteria
Lettuce
Oculatella lusitanica
Phylogeny
Salinity stress
description In this work, a new terrestrial cyanobacterial species, Oculatella lusitanica LEGE 161147, was isolated and characterized using a polyphasic approach. Morphologically, O. lusitanica shares characteristics with different Oculatella species (mainly with O. crustae-formantes), lacking distinctive features. However, the phylogeny based on the 16S rRNA gene sequence and the 16S-23S ITS secondary structures support the establishment of this isolate as a new species. O. lusitanica is placed within a clade mainly composed by other Oculatella terrestrial strains; however, it forms a separate lineage. In addition, our species differs from the other Oculatella described so far by lacking the V2 helix within the ITS region. Since cyanobacteria are known to release compounds that promote plant growth and/or increase their tolerance to stresses, the effect of this newly described cyanobacterial species on Lactuca sativa (lettuce) plants development and salinity stress resistance was evaluated. Our results showed that, although the cyanobacterium had no impact on plant growth under the conditions tested, it was able to mitigate the deleterious salinity stress effects on plant size, root and aerial part fresh weight, by eliciting the non-enzymatic antioxidant response system (proline, H2O2 and reduced glutathione). In addition, the microorganism was able to induce a priming effect on lettuce plants by stimulating defensive mechanisms under non-stress conditions, and enhances the activity of nitrogen metabolism-related enzymes glutamate dehydrogenase, glutamine synthetase and nitrate reductase. These results indicate that this native terrestrial cyanobacterial species could be employed as a tool in sustainable agricultural practices.
publishDate 2022
dc.date.none.fl_str_mv 2022-02-21T15:52:13Z
2022
2022-01-01T00:00:00Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://hdl.handle.net/10198/25087
url http://hdl.handle.net/10198/25087
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv Brito, Ângela; Rocha, Mariana; Kaštovský, Jan; Vieira, Jorge; Vieira, Cristina P.; Ramos, Vitor; Correia, Manuel; Santos, Marina; Mota, Rita; Roque, Jéssica; Pissarra, João; Melo, Paula; Tamagnini, Paula (2022). A new cyanobacterial species with a protective effect on lettuce grown under salinity stress: envisaging sustainable agriculture practices. Journal of Applied Phycology. ISSN 0921-8971. p. 1-14
0921-8971
10.1007/s10811-022-02692-4
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
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