Cyanobacterial nitrogenases: phylogenetic diversity, regulation and functional predictions
Autor(a) principal: | |
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Data de Publicação: | 2016 |
Outros Autores: | , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | LOCUS Repositório Institucional da UFV |
Texto Completo: | http://dx.doi.org/10.1590/1678-4685-gmb-2016-0050 http://www.locus.ufv.br/handle/123456789/12227 |
Resumo: | Cyanobacteria is a remarkable group of prokaryotic photosynthetic microorganisms, with several genera capable of fixing atmospheric nitrogen (N2) and presenting a wide range of morphologies. Although the nitrogenase complex is not present in all cyanobacterial taxa, it is spread across several cyanobacterial strains. The nitrogenase complex has also a high theoretical potential for biofuel production, since H2 is a by-product produced during N2 fixation. In this review we discuss the significance of a relatively wide variety of cell morphologies and metabolic strategies that allow spatial and temporal separation of N2 fixation from photosynthesis in cyanobacteria. Phylogenetic reconstructions based on 16S rRNA and nifD gene sequences shed light on the evolutionary history of the two genes. Our results demonstrated that (i) sequences of genes involved in nitrogen fixation (nifD) from several morphologically distinct strains of cyanobacteria are grouped in similarity with their morphology classification and phylogeny, and (ii) nifD genes from heterocytous strains share a common ancestor. By using this data we also discuss the evolutionary importance of processes such as horizontal gene transfer and genetic duplication for nitrogenase evolution and diversification. Finally, we discuss the importance of H2 synthesis in cyanobacteria, as well as strategies and challenges to improve cyanobacterial H2 production. |
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Esteves-Ferreira, Alberto A.Cavalcanti, João Henrique FrotaVaz, Marcelo Gomes Marçal VieiraAlvarenga, Luna V.Nunes-Nesi, AdrianoAraújo, Wagner L.2017-10-20T10:18:55Z2017-10-20T10:18:55Z2016-12-2116784685http://dx.doi.org/10.1590/1678-4685-gmb-2016-0050http://www.locus.ufv.br/handle/123456789/12227Cyanobacteria is a remarkable group of prokaryotic photosynthetic microorganisms, with several genera capable of fixing atmospheric nitrogen (N2) and presenting a wide range of morphologies. Although the nitrogenase complex is not present in all cyanobacterial taxa, it is spread across several cyanobacterial strains. The nitrogenase complex has also a high theoretical potential for biofuel production, since H2 is a by-product produced during N2 fixation. In this review we discuss the significance of a relatively wide variety of cell morphologies and metabolic strategies that allow spatial and temporal separation of N2 fixation from photosynthesis in cyanobacteria. Phylogenetic reconstructions based on 16S rRNA and nifD gene sequences shed light on the evolutionary history of the two genes. Our results demonstrated that (i) sequences of genes involved in nitrogen fixation (nifD) from several morphologically distinct strains of cyanobacteria are grouped in similarity with their morphology classification and phylogeny, and (ii) nifD genes from heterocytous strains share a common ancestor. By using this data we also discuss the evolutionary importance of processes such as horizontal gene transfer and genetic duplication for nitrogenase evolution and diversification. Finally, we discuss the importance of H2 synthesis in cyanobacteria, as well as strategies and challenges to improve cyanobacterial H2 production.engGenetics and Molecular Biologyvol.40 n.1, 261-275, Mar 2017CyanobacteriaEvolutionHydrogenNitrogen fixationMolecular phylogenyCyanobacterial nitrogenases: phylogenetic diversity, regulation and functional predictionsinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfinfo:eu-repo/semantics/openAccessreponame:LOCUS Repositório Institucional da UFVinstname:Universidade Federal de Viçosa (UFV)instacron:UFVORIGINAL1415-4757-gmb-1678-4685-GMB-2016-0050.pdf1415-4757-gmb-1678-4685-GMB-2016-0050.pdfTexto completoapplication/pdf5035828https://locus.ufv.br//bitstream/123456789/12227/1/1415-4757-gmb-1678-4685-GMB-2016-0050.pdf69a2f9101a5ef6176dff97a68be9cf91MD51LICENSElicense.txtlicense.txttext/plain; charset=utf-81748https://locus.ufv.br//bitstream/123456789/12227/2/license.txt8a4605be74aa9ea9d79846c1fba20a33MD52THUMBNAIL1415-4757-gmb-1678-4685-GMB-2016-0050.pdf.jpg1415-4757-gmb-1678-4685-GMB-2016-0050.pdf.jpgIM Thumbnailimage/jpeg6047https://locus.ufv.br//bitstream/123456789/12227/3/1415-4757-gmb-1678-4685-GMB-2016-0050.pdf.jpgcefde30b13a7cf02e7e9db4df4596685MD53123456789/122272017-10-20 22:00:40.737oai:locus.ufv.br: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Repositório InstitucionalPUBhttps://www.locus.ufv.br/oai/requestfabiojreis@ufv.bropendoar:21452017-10-21T01:00:40LOCUS Repositório Institucional da UFV - Universidade Federal de Viçosa (UFV)false |
dc.title.en.fl_str_mv |
Cyanobacterial nitrogenases: phylogenetic diversity, regulation and functional predictions |
title |
Cyanobacterial nitrogenases: phylogenetic diversity, regulation and functional predictions |
spellingShingle |
Cyanobacterial nitrogenases: phylogenetic diversity, regulation and functional predictions Esteves-Ferreira, Alberto A. Cyanobacteria Evolution Hydrogen Nitrogen fixation Molecular phylogeny |
title_short |
Cyanobacterial nitrogenases: phylogenetic diversity, regulation and functional predictions |
title_full |
Cyanobacterial nitrogenases: phylogenetic diversity, regulation and functional predictions |
title_fullStr |
Cyanobacterial nitrogenases: phylogenetic diversity, regulation and functional predictions |
title_full_unstemmed |
Cyanobacterial nitrogenases: phylogenetic diversity, regulation and functional predictions |
title_sort |
Cyanobacterial nitrogenases: phylogenetic diversity, regulation and functional predictions |
author |
Esteves-Ferreira, Alberto A. |
author_facet |
Esteves-Ferreira, Alberto A. Cavalcanti, João Henrique Frota Vaz, Marcelo Gomes Marçal Vieira Alvarenga, Luna V. Nunes-Nesi, Adriano Araújo, Wagner L. |
author_role |
author |
author2 |
Cavalcanti, João Henrique Frota Vaz, Marcelo Gomes Marçal Vieira Alvarenga, Luna V. Nunes-Nesi, Adriano Araújo, Wagner L. |
author2_role |
author author author author author |
dc.contributor.author.fl_str_mv |
Esteves-Ferreira, Alberto A. Cavalcanti, João Henrique Frota Vaz, Marcelo Gomes Marçal Vieira Alvarenga, Luna V. Nunes-Nesi, Adriano Araújo, Wagner L. |
dc.subject.pt-BR.fl_str_mv |
Cyanobacteria Evolution Hydrogen Nitrogen fixation Molecular phylogeny |
topic |
Cyanobacteria Evolution Hydrogen Nitrogen fixation Molecular phylogeny |
description |
Cyanobacteria is a remarkable group of prokaryotic photosynthetic microorganisms, with several genera capable of fixing atmospheric nitrogen (N2) and presenting a wide range of morphologies. Although the nitrogenase complex is not present in all cyanobacterial taxa, it is spread across several cyanobacterial strains. The nitrogenase complex has also a high theoretical potential for biofuel production, since H2 is a by-product produced during N2 fixation. In this review we discuss the significance of a relatively wide variety of cell morphologies and metabolic strategies that allow spatial and temporal separation of N2 fixation from photosynthesis in cyanobacteria. Phylogenetic reconstructions based on 16S rRNA and nifD gene sequences shed light on the evolutionary history of the two genes. Our results demonstrated that (i) sequences of genes involved in nitrogen fixation (nifD) from several morphologically distinct strains of cyanobacteria are grouped in similarity with their morphology classification and phylogeny, and (ii) nifD genes from heterocytous strains share a common ancestor. By using this data we also discuss the evolutionary importance of processes such as horizontal gene transfer and genetic duplication for nitrogenase evolution and diversification. Finally, we discuss the importance of H2 synthesis in cyanobacteria, as well as strategies and challenges to improve cyanobacterial H2 production. |
publishDate |
2016 |
dc.date.issued.fl_str_mv |
2016-12-21 |
dc.date.accessioned.fl_str_mv |
2017-10-20T10:18:55Z |
dc.date.available.fl_str_mv |
2017-10-20T10:18:55Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.1590/1678-4685-gmb-2016-0050 http://www.locus.ufv.br/handle/123456789/12227 |
dc.identifier.issn.none.fl_str_mv |
16784685 |
identifier_str_mv |
16784685 |
url |
http://dx.doi.org/10.1590/1678-4685-gmb-2016-0050 http://www.locus.ufv.br/handle/123456789/12227 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.ispartofseries.pt-BR.fl_str_mv |
vol.40 n.1, 261-275, Mar 2017 |
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info:eu-repo/semantics/openAccess |
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openAccess |
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Genetics and Molecular Biology |
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Genetics and Molecular Biology |
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