Cre-loxP-based system for removal and reuse of selection markers in Ashbya gossypii targeted engineering

Detalhes bibliográficos
Autor(a) principal: Aguiar, Tatiana Quinta
Data de Publicação: 2014
Outros Autores: Dinis, Cláudia, Domingues, Lucília
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
Texto Completo: http://hdl.handle.net/1822/31659
Resumo: The filamentous ascomycete Ashbya gossypii is amenable to genetic manipulation and is an excellent model system for studying eukaryotic cell biology. However, the number of selection markers in current use for both targeted gene integration and disruption in this fungus are very limited. Therefore, the Cre-loxP recombination system was adapted for use in A. gossypii and its effectiveness in recycling marker genes was demonstrated by constructing both single and double deleted Agura3 and Agade1 auxotrophic strains free of exogenous markers. In spite of its wide use, this is the first report in which the Cre-loxP system was applied to A. gossypii, opening new perspectives for targeted engineering of this fungus with several promising biotechnological applications.
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spelling Cre-loxP-based system for removal and reuse of selection markers in Ashbya gossypii targeted engineeringAshbya gossypiiCre-loxP systemGene targetingMarker recyclingRecombinase expressionScience & TechnologyThe filamentous ascomycete Ashbya gossypii is amenable to genetic manipulation and is an excellent model system for studying eukaryotic cell biology. However, the number of selection markers in current use for both targeted gene integration and disruption in this fungus are very limited. Therefore, the Cre-loxP recombination system was adapted for use in A. gossypii and its effectiveness in recycling marker genes was demonstrated by constructing both single and double deleted Agura3 and Agade1 auxotrophic strains free of exogenous markers. In spite of its wide use, this is the first report in which the Cre-loxP system was applied to A. gossypii, opening new perspectives for targeted engineering of this fungus with several promising biotechnological applications.This work was supported by Fundacao para a Ciencia e a Tecnologia, Portugal, through Project AshByofactory (PTDC/EBB-EBI/101985/2008 - FCOMP-01-0124-FEDER-009701), Project RECI/BBB-EBI/0179/2012 - FCOMP-01-0124-FEDER-027462, Strategic Project PEst-OE/EQB/LA0023/2013, Project BioInd (NORTE-07-0124-FEDER-000028) co-funded by the Programa Operacional Regional do Norte (ON.2 - O Novo Norte), QREN, FEDER, and MIT-Portugal Program PhD Grant SFRH/BD/39112/2007 to Tatiana Q. Aguiar.Academic PressUniversidade do MinhoAguiar, Tatiana QuintaDinis, CláudiaDomingues, Lucília20142014-01-01T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/1822/31659engAguiar, T. Q.; Dinis, C.; Domingues, Lucília, Cre-loxP-based system for removal and reuse of selection markers in Ashbya gossypii targeted engineering. Fungal Genetics and Biology, 68, 1-8, 20141087-18451096-093710.1016/j.fgb.2014.04.00924792968info:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2023-07-21T12:01:06Zoai:repositorium.sdum.uminho.pt:1822/31659Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-19T18:51:01.437488Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse
dc.title.none.fl_str_mv Cre-loxP-based system for removal and reuse of selection markers in Ashbya gossypii targeted engineering
title Cre-loxP-based system for removal and reuse of selection markers in Ashbya gossypii targeted engineering
spellingShingle Cre-loxP-based system for removal and reuse of selection markers in Ashbya gossypii targeted engineering
Aguiar, Tatiana Quinta
Ashbya gossypii
Cre-loxP system
Gene targeting
Marker recycling
Recombinase expression
Science & Technology
title_short Cre-loxP-based system for removal and reuse of selection markers in Ashbya gossypii targeted engineering
title_full Cre-loxP-based system for removal and reuse of selection markers in Ashbya gossypii targeted engineering
title_fullStr Cre-loxP-based system for removal and reuse of selection markers in Ashbya gossypii targeted engineering
title_full_unstemmed Cre-loxP-based system for removal and reuse of selection markers in Ashbya gossypii targeted engineering
title_sort Cre-loxP-based system for removal and reuse of selection markers in Ashbya gossypii targeted engineering
author Aguiar, Tatiana Quinta
author_facet Aguiar, Tatiana Quinta
Dinis, Cláudia
Domingues, Lucília
author_role author
author2 Dinis, Cláudia
Domingues, Lucília
author2_role author
author
dc.contributor.none.fl_str_mv Universidade do Minho
dc.contributor.author.fl_str_mv Aguiar, Tatiana Quinta
Dinis, Cláudia
Domingues, Lucília
dc.subject.por.fl_str_mv Ashbya gossypii
Cre-loxP system
Gene targeting
Marker recycling
Recombinase expression
Science & Technology
topic Ashbya gossypii
Cre-loxP system
Gene targeting
Marker recycling
Recombinase expression
Science & Technology
description The filamentous ascomycete Ashbya gossypii is amenable to genetic manipulation and is an excellent model system for studying eukaryotic cell biology. However, the number of selection markers in current use for both targeted gene integration and disruption in this fungus are very limited. Therefore, the Cre-loxP recombination system was adapted for use in A. gossypii and its effectiveness in recycling marker genes was demonstrated by constructing both single and double deleted Agura3 and Agade1 auxotrophic strains free of exogenous markers. In spite of its wide use, this is the first report in which the Cre-loxP system was applied to A. gossypii, opening new perspectives for targeted engineering of this fungus with several promising biotechnological applications.
publishDate 2014
dc.date.none.fl_str_mv 2014
2014-01-01T00:00:00Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://hdl.handle.net/1822/31659
url http://hdl.handle.net/1822/31659
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv Aguiar, T. Q.; Dinis, C.; Domingues, Lucília, Cre-loxP-based system for removal and reuse of selection markers in Ashbya gossypii targeted engineering. Fungal Genetics and Biology, 68, 1-8, 2014
1087-1845
1096-0937
10.1016/j.fgb.2014.04.009
24792968
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Academic Press
publisher.none.fl_str_mv Academic Press
dc.source.none.fl_str_mv reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron:RCAAP
instname_str Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron_str RCAAP
institution RCAAP
reponame_str Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
collection Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
repository.name.fl_str_mv Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
repository.mail.fl_str_mv
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