Application of the Cre-loxP system for multiple gene disruption in the yeast kluyveromyces marxianus

Detalhes bibliográficos
Autor(a) principal: Ribeiro, Orquídea
Data de Publicação: 2007
Outros Autores: Gombert, Andreas Karoly, Teixeira, J. A., Domingues, Lucília
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
Texto Completo: http://hdl.handle.net/1822/6738
Resumo: The yeast Kluyveromyces marxianus presents several interesting features that make this species a promising industrial yeast for the production of several compounds. In order to take full advantage of this yeast and its particular properties, proper tools for gene disruption and metabolic engineering are needed. The Cre-loxP system is a very versatile tool that allows for gene marker rescue, resulting in mutant strains free of exogenous selective markers, which is a very important aspect for industrial application. As the Cre-loxP system works in some non-conventional yeasts, namely Kluyveromyces lactis, we wished to know whether it also works in K. marxianus. Here, we report the validation of this system in K. marxianus CBS 6556, by disrupting two copies of the LAC4 gene, which encodes a β-galactosidase activity.
id RCAP_f1a3a2cea001bec56bd672c2a3325087
oai_identifier_str oai:repositorium.sdum.uminho.pt:1822/6738
network_acronym_str RCAP
network_name_str Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
repository_id_str 7160
spelling Application of the Cre-loxP system for multiple gene disruption in the yeast kluyveromyces marxianusCre-loxP systemKluyveromyces marxianusGene disruptionβ-GalactosidaseGene targeting efficiencybeta-galactosidaseScience & TechnologyThe yeast Kluyveromyces marxianus presents several interesting features that make this species a promising industrial yeast for the production of several compounds. In order to take full advantage of this yeast and its particular properties, proper tools for gene disruption and metabolic engineering are needed. The Cre-loxP system is a very versatile tool that allows for gene marker rescue, resulting in mutant strains free of exogenous selective markers, which is a very important aspect for industrial application. As the Cre-loxP system works in some non-conventional yeasts, namely Kluyveromyces lactis, we wished to know whether it also works in K. marxianus. Here, we report the validation of this system in K. marxianus CBS 6556, by disrupting two copies of the LAC4 gene, which encodes a β-galactosidase activity.Coordenação de Aperfeiçoamento de Pessoal de Nível Superior - (CAPES), Brazil;Agência de Inovação - projecto UMINHO/POCI-Zimlac/BI/2/05Elsevier B.V.Universidade do MinhoRibeiro, OrquídeaGombert, Andreas KarolyTeixeira, J. A.Domingues, Lucília2007-082007-08-01T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/1822/6738engRIBEIRO, Orquídea [et al.] - Application of the Cre-loxP system for multiple gene disruption in the yeast kluyveromyces marxianus. "Journal of Biotechnology" [Em linha]. 131:1 (Ago. 2007) 20-26. [Consult. 19 Jul. 2007. Disponível em WWW:<URL: http://www.sciencedirect.com/science/journal/01681656. ISSN 0168-1656.0168-165610.1016/j.jbiotec.2007.05.02717624462http://www.sciencedirect.com/info:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2023-07-21T12:17:08Zoai:repositorium.sdum.uminho.pt:1822/6738Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-19T19:09:41.649863Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse
dc.title.none.fl_str_mv Application of the Cre-loxP system for multiple gene disruption in the yeast kluyveromyces marxianus
title Application of the Cre-loxP system for multiple gene disruption in the yeast kluyveromyces marxianus
spellingShingle Application of the Cre-loxP system for multiple gene disruption in the yeast kluyveromyces marxianus
Ribeiro, Orquídea
Cre-loxP system
Kluyveromyces marxianus
Gene disruption
β-Galactosidase
Gene targeting efficiency
beta-galactosidase
Science & Technology
title_short Application of the Cre-loxP system for multiple gene disruption in the yeast kluyveromyces marxianus
title_full Application of the Cre-loxP system for multiple gene disruption in the yeast kluyveromyces marxianus
title_fullStr Application of the Cre-loxP system for multiple gene disruption in the yeast kluyveromyces marxianus
title_full_unstemmed Application of the Cre-loxP system for multiple gene disruption in the yeast kluyveromyces marxianus
title_sort Application of the Cre-loxP system for multiple gene disruption in the yeast kluyveromyces marxianus
author Ribeiro, Orquídea
author_facet Ribeiro, Orquídea
Gombert, Andreas Karoly
Teixeira, J. A.
Domingues, Lucília
author_role author
author2 Gombert, Andreas Karoly
Teixeira, J. A.
Domingues, Lucília
author2_role author
author
author
dc.contributor.none.fl_str_mv Universidade do Minho
dc.contributor.author.fl_str_mv Ribeiro, Orquídea
Gombert, Andreas Karoly
Teixeira, J. A.
Domingues, Lucília
dc.subject.por.fl_str_mv Cre-loxP system
Kluyveromyces marxianus
Gene disruption
β-Galactosidase
Gene targeting efficiency
beta-galactosidase
Science & Technology
topic Cre-loxP system
Kluyveromyces marxianus
Gene disruption
β-Galactosidase
Gene targeting efficiency
beta-galactosidase
Science & Technology
description The yeast Kluyveromyces marxianus presents several interesting features that make this species a promising industrial yeast for the production of several compounds. In order to take full advantage of this yeast and its particular properties, proper tools for gene disruption and metabolic engineering are needed. The Cre-loxP system is a very versatile tool that allows for gene marker rescue, resulting in mutant strains free of exogenous selective markers, which is a very important aspect for industrial application. As the Cre-loxP system works in some non-conventional yeasts, namely Kluyveromyces lactis, we wished to know whether it also works in K. marxianus. Here, we report the validation of this system in K. marxianus CBS 6556, by disrupting two copies of the LAC4 gene, which encodes a β-galactosidase activity.
publishDate 2007
dc.date.none.fl_str_mv 2007-08
2007-08-01T00:00:00Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://hdl.handle.net/1822/6738
url http://hdl.handle.net/1822/6738
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv RIBEIRO, Orquídea [et al.] - Application of the Cre-loxP system for multiple gene disruption in the yeast kluyveromyces marxianus. "Journal of Biotechnology" [Em linha]. 131:1 (Ago. 2007) 20-26. [Consult. 19 Jul. 2007. Disponível em WWW:<URL: http://www.sciencedirect.com/science/journal/01681656. ISSN 0168-1656.
0168-1656
10.1016/j.jbiotec.2007.05.027
17624462
http://www.sciencedirect.com/
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Elsevier B.V.
publisher.none.fl_str_mv Elsevier B.V.
dc.source.none.fl_str_mv reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron:RCAAP
instname_str Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron_str RCAAP
institution RCAAP
reponame_str Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
collection Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
repository.name.fl_str_mv Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
repository.mail.fl_str_mv
_version_ 1799132523572232192

Registros relacionados