Impact of simulated human gastrointestinal digestion on the bioactive fraction of upcycled pineapple by-products
Autor(a) principal: | |
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Data de Publicação: | 2022 |
Outros Autores: | , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
Texto Completo: | http://hdl.handle.net/1822/75351 |
Resumo: | Pineapple by-products (peels and stems) from fruit processing industries were evaluated to understand its potential application as a functional food. Therefore, the bioactive compounds of pineapple by-products were characterized for prebiotic and antioxidant activities. A total characterization of soluble carbohydrates profile (simples and complex carbohydrates), as well as polyphenols was performed, after removal of enzymatic fraction from pineapple crude juice, allowing the decrease of proteolytic activity and improving the other biological activities. Results showed that pineapple liquid fraction, from stem and peels, can be applied as a prebiotic enhancer, promoting the growth of five probiotic microorganisms (two strains of Lactobacillus sp. and three strains of Bifidobacterium sp.), as a single carbohydrate source. Moreover, through HPLC (High Performance Liquid Chromatography) analysis, 10 polyphenols were identified in pineapple liquid fractions, with some expected differences between both evaluated by-products. Gastrointestinal tract was simulated, in a continuous mode to understand the impact of pH changes and gastrointestinal enzymes into pineapple liquid fractions. Results showed a digestion of high molecular weight polysaccharides into small molecular weight tri-, di-, and monosaccharides. There was an increase of samples antioxidant activity through the gastrointestinal stage, followed by the release of specific polyphenols, such as chlorogenic, coumaric, and ferulic acids. The prebiotic activity did not improve throughout the simulation, in fact, the prebiotic potential decreased throughout the different stages. |
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Impact of simulated human gastrointestinal digestion on the bioactive fraction of upcycled pineapple by-productsPineapple by-productsPrebiotic activityAntioxidant activityBioactive compoundsGastrointestinal tract simulationScience & TechnologyPineapple by-products (peels and stems) from fruit processing industries were evaluated to understand its potential application as a functional food. Therefore, the bioactive compounds of pineapple by-products were characterized for prebiotic and antioxidant activities. A total characterization of soluble carbohydrates profile (simples and complex carbohydrates), as well as polyphenols was performed, after removal of enzymatic fraction from pineapple crude juice, allowing the decrease of proteolytic activity and improving the other biological activities. Results showed that pineapple liquid fraction, from stem and peels, can be applied as a prebiotic enhancer, promoting the growth of five probiotic microorganisms (two strains of Lactobacillus sp. and three strains of Bifidobacterium sp.), as a single carbohydrate source. Moreover, through HPLC (High Performance Liquid Chromatography) analysis, 10 polyphenols were identified in pineapple liquid fractions, with some expected differences between both evaluated by-products. Gastrointestinal tract was simulated, in a continuous mode to understand the impact of pH changes and gastrointestinal enzymes into pineapple liquid fractions. Results showed a digestion of high molecular weight polysaccharides into small molecular weight tri-, di-, and monosaccharides. There was an increase of samples antioxidant activity through the gastrointestinal stage, followed by the release of specific polyphenols, such as chlorogenic, coumaric, and ferulic acids. The prebiotic activity did not improve throughout the simulation, in fact, the prebiotic potential decreased throughout the different stages.This research was funded by CBQF under the FCT—Fundação para a Ciência e a Tecnologia through project Multibiorefinery—Multi-purpose strategies for the valorization of a wide range of agro forestry by-products and fisheries: A step forward in the creation of an integrated biorefinery, (POCI-01-0145-FEDER-0066), project UID/Multi/50016/2013 and by Ph.D. grant SFRH/BD/104074/2014 to Débora Campos.info:eu-repo/semantics/publishedVersionMDPIUniversidade do MinhoGómez-García, RicardoVilas-Boas, Ana A.Oliveira, AnaAmorim, ManuelaTeixeira, J. A.Pastrana, LorenzoPintado, Maria ManuelaCampos, Débora A.2022-01-052022-01-05T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/1822/75351engGómez-García, R.; Vilas-Boas, A.A.; Oliveira, A.; Amorim, M.; Teixeira, J.A.; Pastrana, L.; Pintado, M.M.; Campos, D.A. Impact of Simulated Human Gastrointestinal Digestion on the Bioactive Fraction of Upcycled Pineapple By-Products. Foods 2022, 11, 126. https://doi.org/10.3390/foods110101262304-815810.3390/foods11010126https://www.mdpi.com/2304-8158/11/1/126info:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2023-07-21T12:07:48Zoai:repositorium.sdum.uminho.pt:1822/75351Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-19T18:58:51.687561Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse |
dc.title.none.fl_str_mv |
Impact of simulated human gastrointestinal digestion on the bioactive fraction of upcycled pineapple by-products |
title |
Impact of simulated human gastrointestinal digestion on the bioactive fraction of upcycled pineapple by-products |
spellingShingle |
Impact of simulated human gastrointestinal digestion on the bioactive fraction of upcycled pineapple by-products Gómez-García, Ricardo Pineapple by-products Prebiotic activity Antioxidant activity Bioactive compounds Gastrointestinal tract simulation Science & Technology |
title_short |
Impact of simulated human gastrointestinal digestion on the bioactive fraction of upcycled pineapple by-products |
title_full |
Impact of simulated human gastrointestinal digestion on the bioactive fraction of upcycled pineapple by-products |
title_fullStr |
Impact of simulated human gastrointestinal digestion on the bioactive fraction of upcycled pineapple by-products |
title_full_unstemmed |
Impact of simulated human gastrointestinal digestion on the bioactive fraction of upcycled pineapple by-products |
title_sort |
Impact of simulated human gastrointestinal digestion on the bioactive fraction of upcycled pineapple by-products |
author |
Gómez-García, Ricardo |
author_facet |
Gómez-García, Ricardo Vilas-Boas, Ana A. Oliveira, Ana Amorim, Manuela Teixeira, J. A. Pastrana, Lorenzo Pintado, Maria Manuela Campos, Débora A. |
author_role |
author |
author2 |
Vilas-Boas, Ana A. Oliveira, Ana Amorim, Manuela Teixeira, J. A. Pastrana, Lorenzo Pintado, Maria Manuela Campos, Débora A. |
author2_role |
author author author author author author author |
dc.contributor.none.fl_str_mv |
Universidade do Minho |
dc.contributor.author.fl_str_mv |
Gómez-García, Ricardo Vilas-Boas, Ana A. Oliveira, Ana Amorim, Manuela Teixeira, J. A. Pastrana, Lorenzo Pintado, Maria Manuela Campos, Débora A. |
dc.subject.por.fl_str_mv |
Pineapple by-products Prebiotic activity Antioxidant activity Bioactive compounds Gastrointestinal tract simulation Science & Technology |
topic |
Pineapple by-products Prebiotic activity Antioxidant activity Bioactive compounds Gastrointestinal tract simulation Science & Technology |
description |
Pineapple by-products (peels and stems) from fruit processing industries were evaluated to understand its potential application as a functional food. Therefore, the bioactive compounds of pineapple by-products were characterized for prebiotic and antioxidant activities. A total characterization of soluble carbohydrates profile (simples and complex carbohydrates), as well as polyphenols was performed, after removal of enzymatic fraction from pineapple crude juice, allowing the decrease of proteolytic activity and improving the other biological activities. Results showed that pineapple liquid fraction, from stem and peels, can be applied as a prebiotic enhancer, promoting the growth of five probiotic microorganisms (two strains of Lactobacillus sp. and three strains of Bifidobacterium sp.), as a single carbohydrate source. Moreover, through HPLC (High Performance Liquid Chromatography) analysis, 10 polyphenols were identified in pineapple liquid fractions, with some expected differences between both evaluated by-products. Gastrointestinal tract was simulated, in a continuous mode to understand the impact of pH changes and gastrointestinal enzymes into pineapple liquid fractions. Results showed a digestion of high molecular weight polysaccharides into small molecular weight tri-, di-, and monosaccharides. There was an increase of samples antioxidant activity through the gastrointestinal stage, followed by the release of specific polyphenols, such as chlorogenic, coumaric, and ferulic acids. The prebiotic activity did not improve throughout the simulation, in fact, the prebiotic potential decreased throughout the different stages. |
publishDate |
2022 |
dc.date.none.fl_str_mv |
2022-01-05 2022-01-05T00:00:00Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://hdl.handle.net/1822/75351 |
url |
http://hdl.handle.net/1822/75351 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Gómez-García, R.; Vilas-Boas, A.A.; Oliveira, A.; Amorim, M.; Teixeira, J.A.; Pastrana, L.; Pintado, M.M.; Campos, D.A. Impact of Simulated Human Gastrointestinal Digestion on the Bioactive Fraction of Upcycled Pineapple By-Products. Foods 2022, 11, 126. https://doi.org/10.3390/foods11010126 2304-8158 10.3390/foods11010126 https://www.mdpi.com/2304-8158/11/1/126 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.publisher.none.fl_str_mv |
MDPI |
publisher.none.fl_str_mv |
MDPI |
dc.source.none.fl_str_mv |
reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação instacron:RCAAP |
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Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
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RCAAP |
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Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
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Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
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Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
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