Pulmonary toxicity and gene expression changes after short-term inhalation exposure to surface-modified copper oxide nanoparticles

Detalhes bibliográficos
Autor(a) principal: Gosens, Ilse
Data de Publicação: 2021
Outros Autores: Costa, Pedro M., Olsson, Magnus, Stone, Vicki, Costa, Anna L., Brunelli, Andrea, Badetti, Elena, Bonetto, Alessandro, Bokkers, Bas G. H., de Jong, Wim H., Williams, Andrew, Halappanavar, Sabina, Fadeel, Bengt, Cassee, Flemming R.
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
Texto Completo: http://hdl.handle.net/10362/128469
Resumo: SPR, E/121504
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spelling Pulmonary toxicity and gene expression changes after short-term inhalation exposure to surface-modified copper oxide nanoparticlesCopper oxideNanoparticlesPulmonary toxicitySafe-by-designTranscriptomicsMaterials Science (miscellaneous)Safety, Risk, Reliability and QualitySafety ResearchPublic Health, Environmental and Occupational HealthSDG 3 - Good Health and Well-beingSPR, E/121504Copper oxide nanoparticles (CuO NPs) have previously been shown to cause dose-dependent pulmonary toxicity following inhalation. Here, CuO NPs (10 nm), coated with polyethylenimine (PEI) or ascorbate (ASC) resulting in positively or negatively charged NPs, respectively, were evaluated. Rats were exposed nose-only to similar exposure dose levels of ASC or PEI coated CuO NPs for 5 consecutive days. On day 6 and day 27 post-exposure, pulmonary toxicity markers in bronchoalveolar lavage fluid (BALF), lung histopathology and genome-wide transcriptomic changes in lungs, were assessed. BALF analyses showed a dose-dependent pulmonary inflammation and cell damage, which was supported by the lung histopathological findings of hypertrophy/hyperplasia of bronchiolar and alveolar epithelium, interstitial and alveolar inflammation, and paracortical histiocytosis in mediastinal lymph nodes for both types of CuO NPs. Transcriptomics analysis showed that pathways related to inflammation and cell proliferation were significantly activated. Additionally, we found evidence for the dysregulation of drug metabolism-related genes, especially in rats exposed to ASC-coated CuO NPs. Overall, no differences in the type of toxic effects and potency between the two surface coatings could be established, except with respect to the (regional) dose that initiates bronchiolar and alveolar hypertrophy. This disproves our hypothesis that differences in surface coatings affect the pulmonary toxicity of CuO NPs.DCV - Departamento de Ciências da VidaUCIBIO - Applied Molecular Biosciences UnitRUNGosens, IlseCosta, Pedro M.Olsson, MagnusStone, VickiCosta, Anna L.Brunelli, AndreaBadetti, ElenaBonetto, AlessandroBokkers, Bas G. H.de Jong, Wim H.Williams, AndrewHalappanavar, SabinaFadeel, BengtCassee, Flemming R.2021-11-29T23:40:04Z2021-042021-04-01T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/10362/128469eng2452-0748PURE: 35107942https://doi.org/10.1016/j.impact.2021.100313info:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2024-03-11T05:08:03Zoai:run.unl.pt:10362/128469Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-20T03:46:19.040141Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse
dc.title.none.fl_str_mv Pulmonary toxicity and gene expression changes after short-term inhalation exposure to surface-modified copper oxide nanoparticles
title Pulmonary toxicity and gene expression changes after short-term inhalation exposure to surface-modified copper oxide nanoparticles
spellingShingle Pulmonary toxicity and gene expression changes after short-term inhalation exposure to surface-modified copper oxide nanoparticles
Gosens, Ilse
Copper oxide
Nanoparticles
Pulmonary toxicity
Safe-by-design
Transcriptomics
Materials Science (miscellaneous)
Safety, Risk, Reliability and Quality
Safety Research
Public Health, Environmental and Occupational Health
SDG 3 - Good Health and Well-being
title_short Pulmonary toxicity and gene expression changes after short-term inhalation exposure to surface-modified copper oxide nanoparticles
title_full Pulmonary toxicity and gene expression changes after short-term inhalation exposure to surface-modified copper oxide nanoparticles
title_fullStr Pulmonary toxicity and gene expression changes after short-term inhalation exposure to surface-modified copper oxide nanoparticles
title_full_unstemmed Pulmonary toxicity and gene expression changes after short-term inhalation exposure to surface-modified copper oxide nanoparticles
title_sort Pulmonary toxicity and gene expression changes after short-term inhalation exposure to surface-modified copper oxide nanoparticles
author Gosens, Ilse
author_facet Gosens, Ilse
Costa, Pedro M.
Olsson, Magnus
Stone, Vicki
Costa, Anna L.
Brunelli, Andrea
Badetti, Elena
Bonetto, Alessandro
Bokkers, Bas G. H.
de Jong, Wim H.
Williams, Andrew
Halappanavar, Sabina
Fadeel, Bengt
Cassee, Flemming R.
author_role author
author2 Costa, Pedro M.
Olsson, Magnus
Stone, Vicki
Costa, Anna L.
Brunelli, Andrea
Badetti, Elena
Bonetto, Alessandro
Bokkers, Bas G. H.
de Jong, Wim H.
Williams, Andrew
Halappanavar, Sabina
Fadeel, Bengt
Cassee, Flemming R.
author2_role author
author
author
author
author
author
author
author
author
author
author
author
author
dc.contributor.none.fl_str_mv DCV - Departamento de Ciências da Vida
UCIBIO - Applied Molecular Biosciences Unit
RUN
dc.contributor.author.fl_str_mv Gosens, Ilse
Costa, Pedro M.
Olsson, Magnus
Stone, Vicki
Costa, Anna L.
Brunelli, Andrea
Badetti, Elena
Bonetto, Alessandro
Bokkers, Bas G. H.
de Jong, Wim H.
Williams, Andrew
Halappanavar, Sabina
Fadeel, Bengt
Cassee, Flemming R.
dc.subject.por.fl_str_mv Copper oxide
Nanoparticles
Pulmonary toxicity
Safe-by-design
Transcriptomics
Materials Science (miscellaneous)
Safety, Risk, Reliability and Quality
Safety Research
Public Health, Environmental and Occupational Health
SDG 3 - Good Health and Well-being
topic Copper oxide
Nanoparticles
Pulmonary toxicity
Safe-by-design
Transcriptomics
Materials Science (miscellaneous)
Safety, Risk, Reliability and Quality
Safety Research
Public Health, Environmental and Occupational Health
SDG 3 - Good Health and Well-being
description SPR, E/121504
publishDate 2021
dc.date.none.fl_str_mv 2021-11-29T23:40:04Z
2021-04
2021-04-01T00:00:00Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://hdl.handle.net/10362/128469
url http://hdl.handle.net/10362/128469
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv 2452-0748
PURE: 35107942
https://doi.org/10.1016/j.impact.2021.100313
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.source.none.fl_str_mv reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron:RCAAP
instname_str Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron_str RCAAP
institution RCAAP
reponame_str Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
collection Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
repository.name.fl_str_mv Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
repository.mail.fl_str_mv
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