Molecular tools to dissect the role of Dmrt2a and Dmrt2b in the left-right axis formation in zebrafish

Detalhes bibliográficos
Autor(a) principal: Santos, José Guilherme Pereira de Almeida
Data de Publicação: 2013
Tipo de documento: Dissertação
Idioma: eng
Título da fonte: Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
Texto Completo: http://hdl.handle.net/10362/10902
Resumo: Dissertação para obtenção do Grau de Mestre em Genética Molecular e Biomedicina
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spelling Molecular tools to dissect the role of Dmrt2a and Dmrt2b in the left-right axis formation in zebrafishDmrt2aDmrt2bLeft-rightSymmetryAsymmetryTALENsDissertação para obtenção do Grau de Mestre em Genética Molecular e BiomedicinaWe tend to view the vertebrate body as bilaterally symmetric, but in fact, this only happens from the outside. Internally, most of the organs from heart to liver are asymmetrically positioned. Skeleton and its associated muscles, symmetric structures of the vertebrate body, have its origins in the transient symmetric blocks of mesoderm called somites whereas the asymmetric morphogenesis of the internal organs is due to asymmetric gene expression in the lateral plate mesoderm (LPM). Previous studies using Morpholino (MO) technology have shown that dmrt2a is involved in these two processes in zebrafish. When Dmrt2a levels are reduced, asymmetric gene expression in the LPM becomes randomized and symmetric gene expression in the presomitic mesoderm (PSM) is disrupted. The paralogous of dmrt2a, the fish specific dmrt2b has been shown to be involved in regulating asymmetric gene expression in the LPM as well. Here we used the recent Transcription activator-like effector nucleases (TALENs) technology to generate dmrt2a and dmrt2b mutant alleles that will allow us in the future to uncover the downstream effectors of these transcription factors using high-throughput experiments. In addition, we overexpressed dmrt2a at the one-cell stage to characterize asymmetry versus symmetry phenotypes. The results show clearly the ability of TALEN technology to generate mutant alleles in zebrafish. Nevertheless, dmrt2a and dmrt2b homozygous mutants developed so far fail to recapitulate their previously described MO phenotypes which raise the question on what molecular mechanism(s) allow(s) zebrafish to cope with frameshift mutations. The overexpression of dmrt2a shows that a time window of opportunity during which symmetric embryonic territories are able to respond to asymmetric signals does exist during embryonic development.Faculdade de Ciências e TecnologiaSaúde, Maria LeonorRUNSantos, José Guilherme Pereira de Almeida2013-12-19T15:19:53Z20132013-01-01T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/masterThesisapplication/pdfhttp://hdl.handle.net/10362/10902enginfo:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2024-03-11T03:44:57Zoai:run.unl.pt:10362/10902Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-20T03:19:49.844859Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse
dc.title.none.fl_str_mv Molecular tools to dissect the role of Dmrt2a and Dmrt2b in the left-right axis formation in zebrafish
title Molecular tools to dissect the role of Dmrt2a and Dmrt2b in the left-right axis formation in zebrafish
spellingShingle Molecular tools to dissect the role of Dmrt2a and Dmrt2b in the left-right axis formation in zebrafish
Santos, José Guilherme Pereira de Almeida
Dmrt2a
Dmrt2b
Left-right
Symmetry
Asymmetry
TALENs
title_short Molecular tools to dissect the role of Dmrt2a and Dmrt2b in the left-right axis formation in zebrafish
title_full Molecular tools to dissect the role of Dmrt2a and Dmrt2b in the left-right axis formation in zebrafish
title_fullStr Molecular tools to dissect the role of Dmrt2a and Dmrt2b in the left-right axis formation in zebrafish
title_full_unstemmed Molecular tools to dissect the role of Dmrt2a and Dmrt2b in the left-right axis formation in zebrafish
title_sort Molecular tools to dissect the role of Dmrt2a and Dmrt2b in the left-right axis formation in zebrafish
author Santos, José Guilherme Pereira de Almeida
author_facet Santos, José Guilherme Pereira de Almeida
author_role author
dc.contributor.none.fl_str_mv Saúde, Maria Leonor
RUN
dc.contributor.author.fl_str_mv Santos, José Guilherme Pereira de Almeida
dc.subject.por.fl_str_mv Dmrt2a
Dmrt2b
Left-right
Symmetry
Asymmetry
TALENs
topic Dmrt2a
Dmrt2b
Left-right
Symmetry
Asymmetry
TALENs
description Dissertação para obtenção do Grau de Mestre em Genética Molecular e Biomedicina
publishDate 2013
dc.date.none.fl_str_mv 2013-12-19T15:19:53Z
2013
2013-01-01T00:00:00Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/masterThesis
format masterThesis
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://hdl.handle.net/10362/10902
url http://hdl.handle.net/10362/10902
dc.language.iso.fl_str_mv eng
language eng
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Faculdade de Ciências e Tecnologia
publisher.none.fl_str_mv Faculdade de Ciências e Tecnologia
dc.source.none.fl_str_mv reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron:RCAAP
instname_str Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron_str RCAAP
institution RCAAP
reponame_str Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
collection Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
repository.name.fl_str_mv Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
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