Identification of Dmrt2a downstream genes during zebrafish early development using a timely controlled approach

Detalhes bibliográficos
Autor(a) principal: Pinto, Rita Alexandra
Data de Publicação: 2018
Outros Autores: Almeida-Santos, José, Lourenço, Raquel, Saúde, Leonor
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
Texto Completo: http://hdl.handle.net/10362/40735
Resumo: This research was supported by FCT (Portugal) grant (PTDC/SAU-BID/119627/2010) given to L.S. L.S. was supported by an IF contract from FCT (Portugal). R.A.P. was supported by a PhD fellowship (SFRH/BD/87607/2012) from FCT (Portugal). Publication was sponsored by LISBOA-01-0145-FEDER-007391, project co-funded by FEDER through POR Lisboa 2020 - Programa Operacional Regional de Lisboa, PORTUGAL 2020 and by Fundacao para a Ciencia e a Tecnologia.
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spelling Identification of Dmrt2a downstream genes during zebrafish early development using a timely controlled approachDmrt2admrt2a transgenicDmrt2bTALEN mutants,MicroarraysZebrafishThis research was supported by FCT (Portugal) grant (PTDC/SAU-BID/119627/2010) given to L.S. L.S. was supported by an IF contract from FCT (Portugal). R.A.P. was supported by a PhD fellowship (SFRH/BD/87607/2012) from FCT (Portugal). Publication was sponsored by LISBOA-01-0145-FEDER-007391, project co-funded by FEDER through POR Lisboa 2020 - Programa Operacional Regional de Lisboa, PORTUGAL 2020 and by Fundacao para a Ciencia e a Tecnologia.BACKGROUND: Dmrt2a is a zinc finger like transcription factor with several roles during zebrafish early development: left-right asymmetry, synchronisation of the somite clock genes and fast muscle differentiation. Despite the described functions, Dmrt2a mechanism of action is unknown. Therefore, with this work, we propose to identify Dmrt2a downstream genes during zebrafish early development. RESULTS: We generated and validated a heat-shock inducible transgenic line, to timely control dmrt2a overexpression, and dmrt2a mutant lines. We characterised dmrt2a overexpression phenotype and verified that it was very similar to the one described after knockdown of this gene, with left-right asymmetry defects and desynchronisation of somite clock genes. Additionally, we identified a new phenotype of somite border malformation. We generated several dmrt2a mutant lines, but we only detected a weak to negligible phenotype. As dmrt2a has a paralog gene, dmrt2b, with similar functions and expression pattern, we evaluated the possibility of redundancy. We found that dmrt2b does not seem to compensate the lack of dmrt2a. Furthermore, we took advantage of one of our mutant lines to confirm dmrt2a morpholino specificity, which was previously shown to be a robust knockdown tool in two independent studies. Using the described genetic tools to perform and validate a microarray, we were able to identify six genes downstream of Dmrt2a: foxj1b, pxdc1b, cxcl12b, etv2, foxc1b and cyp1a. CONCLUSIONS: In this work, we generated and validated several genetic tools for dmrt2a and identified six genes downstream of this transcription factor. The identified genes will be crucial to the future understanding of Dmrt2a mechanism of action in zebrafish.NOVA Medical School|Faculdade de Ciências Médicas (NMS|FCM)Centro de Estudos de Doenças Crónicas (CEDOC)RUNPinto, Rita AlexandraAlmeida-Santos, JoséLourenço, RaquelSaúde, Leonor2018-07-02T22:11:05Z2018-06-192018-06-19T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/10362/40735eng1471-213XPURE: 4443042https://doi.org/10.1186/s12861-018-0173-5info:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2024-03-11T04:22:06Zoai:run.unl.pt:10362/40735Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-20T03:31:16.613991Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse
dc.title.none.fl_str_mv Identification of Dmrt2a downstream genes during zebrafish early development using a timely controlled approach
title Identification of Dmrt2a downstream genes during zebrafish early development using a timely controlled approach
spellingShingle Identification of Dmrt2a downstream genes during zebrafish early development using a timely controlled approach
Pinto, Rita Alexandra
Dmrt2a
dmrt2a transgenic
Dmrt2b
TALEN mutants,
Microarrays
Zebrafish
title_short Identification of Dmrt2a downstream genes during zebrafish early development using a timely controlled approach
title_full Identification of Dmrt2a downstream genes during zebrafish early development using a timely controlled approach
title_fullStr Identification of Dmrt2a downstream genes during zebrafish early development using a timely controlled approach
title_full_unstemmed Identification of Dmrt2a downstream genes during zebrafish early development using a timely controlled approach
title_sort Identification of Dmrt2a downstream genes during zebrafish early development using a timely controlled approach
author Pinto, Rita Alexandra
author_facet Pinto, Rita Alexandra
Almeida-Santos, José
Lourenço, Raquel
Saúde, Leonor
author_role author
author2 Almeida-Santos, José
Lourenço, Raquel
Saúde, Leonor
author2_role author
author
author
dc.contributor.none.fl_str_mv NOVA Medical School|Faculdade de Ciências Médicas (NMS|FCM)
Centro de Estudos de Doenças Crónicas (CEDOC)
RUN
dc.contributor.author.fl_str_mv Pinto, Rita Alexandra
Almeida-Santos, José
Lourenço, Raquel
Saúde, Leonor
dc.subject.por.fl_str_mv Dmrt2a
dmrt2a transgenic
Dmrt2b
TALEN mutants,
Microarrays
Zebrafish
topic Dmrt2a
dmrt2a transgenic
Dmrt2b
TALEN mutants,
Microarrays
Zebrafish
description This research was supported by FCT (Portugal) grant (PTDC/SAU-BID/119627/2010) given to L.S. L.S. was supported by an IF contract from FCT (Portugal). R.A.P. was supported by a PhD fellowship (SFRH/BD/87607/2012) from FCT (Portugal). Publication was sponsored by LISBOA-01-0145-FEDER-007391, project co-funded by FEDER through POR Lisboa 2020 - Programa Operacional Regional de Lisboa, PORTUGAL 2020 and by Fundacao para a Ciencia e a Tecnologia.
publishDate 2018
dc.date.none.fl_str_mv 2018-07-02T22:11:05Z
2018-06-19
2018-06-19T00:00:00Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://hdl.handle.net/10362/40735
url http://hdl.handle.net/10362/40735
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv 1471-213X
PURE: 4443042
https://doi.org/10.1186/s12861-018-0173-5
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.source.none.fl_str_mv reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron:RCAAP
instname_str Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron_str RCAAP
institution RCAAP
reponame_str Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
collection Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
repository.name.fl_str_mv Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
repository.mail.fl_str_mv
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