PACT/PRKRA and p53 regulate transcriptional activity of DMRT1

Detalhes bibliográficos
Autor(a) principal: Fujitani,Kazuko
Data de Publicação: 2020
Outros Autores: Otomo,Asako, Nagayama,Yuto, Tachibana,Taro, Kato,Rika, Kawashima,Yusuke, Kodera,Yoshio, Kato,Tomoko, Takada,Shuji, Tamura,Kei, Takamatsu,Nobuhiko, Ito,Michihiko
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Genetics and Molecular Biology
Texto Completo: http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572020000400702
Resumo: Abstract The transcription factor DMRT1 (doublesex and mab-3 related transcription factor) has two distinct functions, somatic-cell masculinization and germ-cell development in some vertebrate species, including mouse and the African clawed frog Xenopus laevis. However, its transcriptional regulation remains unclear. We tried to identify DMRT1-interacting proteins from X. laevis testes by immunoprecipitation with an anti-DMRT1 antibody and MS/MS analysis, and selected three proteins, including PACT/PRKRA (Interferon-inducible double-stranded RNA dependent protein kinase activator A) derived from testes. Next, we examined the effects of PACT/PRKRA and/or p53 on the transcriptional activity of DMRT1. In transfected 293T cells, PACT/PRKRA and p53 significantly enhanced and repressed DMRT1-driven luciferase activity, respectively. We also observed that the enhanced activity by PACT/PRKRA was strongly attenuated by p53. Moreover, in situ hybridization analysis of Pact/Prkra mRNA in tadpole gonads indicated high expression in female and male germline stem cells. Taken together, these findings suggest that PACT/PRKRA and p53 might positively and negatively regulate the activity of DMRT1, respectively, for germline stem cell fate.
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spelling PACT/PRKRA and p53 regulate transcriptional activity of DMRT1p53DMRT1germline stem celltestisXenopusAbstract The transcription factor DMRT1 (doublesex and mab-3 related transcription factor) has two distinct functions, somatic-cell masculinization and germ-cell development in some vertebrate species, including mouse and the African clawed frog Xenopus laevis. However, its transcriptional regulation remains unclear. We tried to identify DMRT1-interacting proteins from X. laevis testes by immunoprecipitation with an anti-DMRT1 antibody and MS/MS analysis, and selected three proteins, including PACT/PRKRA (Interferon-inducible double-stranded RNA dependent protein kinase activator A) derived from testes. Next, we examined the effects of PACT/PRKRA and/or p53 on the transcriptional activity of DMRT1. In transfected 293T cells, PACT/PRKRA and p53 significantly enhanced and repressed DMRT1-driven luciferase activity, respectively. We also observed that the enhanced activity by PACT/PRKRA was strongly attenuated by p53. Moreover, in situ hybridization analysis of Pact/Prkra mRNA in tadpole gonads indicated high expression in female and male germline stem cells. Taken together, these findings suggest that PACT/PRKRA and p53 might positively and negatively regulate the activity of DMRT1, respectively, for germline stem cell fate.Sociedade Brasileira de Genética2020-01-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572020000400702Genetics and Molecular Biology v.43 n.2 2020reponame:Genetics and Molecular Biologyinstname:Sociedade Brasileira de Genética (SBG)instacron:SBG10.1590/1678-4685-gmb-2019-0017info:eu-repo/semantics/openAccessFujitani,KazukoOtomo,AsakoNagayama,YutoTachibana,TaroKato,RikaKawashima,YusukeKodera,YoshioKato,TomokoTakada,ShujiTamura,KeiTakamatsu,NobuhikoIto,Michihikoeng2020-04-02T00:00:00Zoai:scielo:S1415-47572020000400702Revistahttp://www.gmb.org.br/ONGhttps://old.scielo.br/oai/scielo-oai.php||editor@gmb.org.br1678-46851415-4757opendoar:2020-04-02T00:00Genetics and Molecular Biology - Sociedade Brasileira de Genética (SBG)false
dc.title.none.fl_str_mv PACT/PRKRA and p53 regulate transcriptional activity of DMRT1
title PACT/PRKRA and p53 regulate transcriptional activity of DMRT1
spellingShingle PACT/PRKRA and p53 regulate transcriptional activity of DMRT1
Fujitani,Kazuko
p53
DMRT1
germline stem cell
testis
Xenopus
title_short PACT/PRKRA and p53 regulate transcriptional activity of DMRT1
title_full PACT/PRKRA and p53 regulate transcriptional activity of DMRT1
title_fullStr PACT/PRKRA and p53 regulate transcriptional activity of DMRT1
title_full_unstemmed PACT/PRKRA and p53 regulate transcriptional activity of DMRT1
title_sort PACT/PRKRA and p53 regulate transcriptional activity of DMRT1
author Fujitani,Kazuko
author_facet Fujitani,Kazuko
Otomo,Asako
Nagayama,Yuto
Tachibana,Taro
Kato,Rika
Kawashima,Yusuke
Kodera,Yoshio
Kato,Tomoko
Takada,Shuji
Tamura,Kei
Takamatsu,Nobuhiko
Ito,Michihiko
author_role author
author2 Otomo,Asako
Nagayama,Yuto
Tachibana,Taro
Kato,Rika
Kawashima,Yusuke
Kodera,Yoshio
Kato,Tomoko
Takada,Shuji
Tamura,Kei
Takamatsu,Nobuhiko
Ito,Michihiko
author2_role author
author
author
author
author
author
author
author
author
author
author
dc.contributor.author.fl_str_mv Fujitani,Kazuko
Otomo,Asako
Nagayama,Yuto
Tachibana,Taro
Kato,Rika
Kawashima,Yusuke
Kodera,Yoshio
Kato,Tomoko
Takada,Shuji
Tamura,Kei
Takamatsu,Nobuhiko
Ito,Michihiko
dc.subject.por.fl_str_mv p53
DMRT1
germline stem cell
testis
Xenopus
topic p53
DMRT1
germline stem cell
testis
Xenopus
description Abstract The transcription factor DMRT1 (doublesex and mab-3 related transcription factor) has two distinct functions, somatic-cell masculinization and germ-cell development in some vertebrate species, including mouse and the African clawed frog Xenopus laevis. However, its transcriptional regulation remains unclear. We tried to identify DMRT1-interacting proteins from X. laevis testes by immunoprecipitation with an anti-DMRT1 antibody and MS/MS analysis, and selected three proteins, including PACT/PRKRA (Interferon-inducible double-stranded RNA dependent protein kinase activator A) derived from testes. Next, we examined the effects of PACT/PRKRA and/or p53 on the transcriptional activity of DMRT1. In transfected 293T cells, PACT/PRKRA and p53 significantly enhanced and repressed DMRT1-driven luciferase activity, respectively. We also observed that the enhanced activity by PACT/PRKRA was strongly attenuated by p53. Moreover, in situ hybridization analysis of Pact/Prkra mRNA in tadpole gonads indicated high expression in female and male germline stem cells. Taken together, these findings suggest that PACT/PRKRA and p53 might positively and negatively regulate the activity of DMRT1, respectively, for germline stem cell fate.
publishDate 2020
dc.date.none.fl_str_mv 2020-01-01
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572020000400702
url http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572020000400702
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv 10.1590/1678-4685-gmb-2019-0017
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv text/html
dc.publisher.none.fl_str_mv Sociedade Brasileira de Genética
publisher.none.fl_str_mv Sociedade Brasileira de Genética
dc.source.none.fl_str_mv Genetics and Molecular Biology v.43 n.2 2020
reponame:Genetics and Molecular Biology
instname:Sociedade Brasileira de Genética (SBG)
instacron:SBG
instname_str Sociedade Brasileira de Genética (SBG)
instacron_str SBG
institution SBG
reponame_str Genetics and Molecular Biology
collection Genetics and Molecular Biology
repository.name.fl_str_mv Genetics and Molecular Biology - Sociedade Brasileira de Genética (SBG)
repository.mail.fl_str_mv ||editor@gmb.org.br
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