Osteogenic induction of hBMSCs by electrospun scaffolds with dexamethasone release functionality
Autor(a) principal: | |
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Data de Publicação: | 2010 |
Outros Autores: | , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
Texto Completo: | http://hdl.handle.net/1822/20565 |
Resumo: | Electrospun structures were proposed as scaffolds owing to their morphological and structural similarities with the extracellular matrix found in many native tissues. These !brous structures were also proposed as drug release systems by exploiting the direct dependence of the release rate of a drug on the surface area. An osteogenic differentiation factor, dexamethasone (DEX), was incorporated into electrospun polycaprolactone (PCL) nano!bers at different concentrations (5, 10, 15 and 20 wt.% polymer), in a single-step process. The DEX incorporated into the polymeric carrier is in amorphous state, as determined by DSC, and does not in"uence the typical nano!bers morphology. In vitro drug release studies demonstrated that the dexamethasone release was sustained over a period of 15 days. The bioactivity of the released dexamethasone was assessed by cultivating human bone marrow mesenchymal stem cells (hBMSCs) on 15 wt.% DEX-loaded PCL NFMs, under dexamethasone-absent osteogenic differentiation medium formulation. An increased concentration of alkaline phosphatase and deposition of a mineralized matrix was observed. Phenotypic and genotypic expression of osteoblastic-speci!c markers corroborates the osteogenic activity of the loaded growth/differentiation factor. Overall data suggests that the electrospun biodegradable nano!bers can be used as carriers for the sustained release of growth/differentiation factors relevant for bone tissue engineering strategies. |
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Osteogenic induction of hBMSCs by electrospun scaffolds with dexamethasone release functionalityDrug releasePolycaprolactoneMesenchymal stem cellsBone tissue engineeringMolecular biologyScience & TechnologyElectrospun structures were proposed as scaffolds owing to their morphological and structural similarities with the extracellular matrix found in many native tissues. These !brous structures were also proposed as drug release systems by exploiting the direct dependence of the release rate of a drug on the surface area. An osteogenic differentiation factor, dexamethasone (DEX), was incorporated into electrospun polycaprolactone (PCL) nano!bers at different concentrations (5, 10, 15 and 20 wt.% polymer), in a single-step process. The DEX incorporated into the polymeric carrier is in amorphous state, as determined by DSC, and does not in"uence the typical nano!bers morphology. In vitro drug release studies demonstrated that the dexamethasone release was sustained over a period of 15 days. The bioactivity of the released dexamethasone was assessed by cultivating human bone marrow mesenchymal stem cells (hBMSCs) on 15 wt.% DEX-loaded PCL NFMs, under dexamethasone-absent osteogenic differentiation medium formulation. An increased concentration of alkaline phosphatase and deposition of a mineralized matrix was observed. Phenotypic and genotypic expression of osteoblastic-speci!c markers corroborates the osteogenic activity of the loaded growth/differentiation factor. Overall data suggests that the electrospun biodegradable nano!bers can be used as carriers for the sustained release of growth/differentiation factors relevant for bone tissue engineering strategies.This work was partially supported by the European Network of Excellence EXPERTISSUES (NMP3-CT-2004-500283). The Portuguese Foundation for Science and Technology was acknowledged for the PhD grant of A. Martins (SFRH/BD/24382/2005).ElsevierUniversidade do MinhoMartins, AlbinoDuarte, Ana Rita C.Faria, SusanaMarques, A. P.Reis, R. L.Neves, N. M.20102010-01-01T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/1822/20565eng0142-961210.1016/j.biomaterials.2010.04.01020452016info:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2023-07-21T12:47:41Zoai:repositorium.sdum.uminho.pt:1822/20565Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-19T19:45:47.131778Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse |
dc.title.none.fl_str_mv |
Osteogenic induction of hBMSCs by electrospun scaffolds with dexamethasone release functionality |
title |
Osteogenic induction of hBMSCs by electrospun scaffolds with dexamethasone release functionality |
spellingShingle |
Osteogenic induction of hBMSCs by electrospun scaffolds with dexamethasone release functionality Martins, Albino Drug release Polycaprolactone Mesenchymal stem cells Bone tissue engineering Molecular biology Science & Technology |
title_short |
Osteogenic induction of hBMSCs by electrospun scaffolds with dexamethasone release functionality |
title_full |
Osteogenic induction of hBMSCs by electrospun scaffolds with dexamethasone release functionality |
title_fullStr |
Osteogenic induction of hBMSCs by electrospun scaffolds with dexamethasone release functionality |
title_full_unstemmed |
Osteogenic induction of hBMSCs by electrospun scaffolds with dexamethasone release functionality |
title_sort |
Osteogenic induction of hBMSCs by electrospun scaffolds with dexamethasone release functionality |
author |
Martins, Albino |
author_facet |
Martins, Albino Duarte, Ana Rita C. Faria, Susana Marques, A. P. Reis, R. L. Neves, N. M. |
author_role |
author |
author2 |
Duarte, Ana Rita C. Faria, Susana Marques, A. P. Reis, R. L. Neves, N. M. |
author2_role |
author author author author author |
dc.contributor.none.fl_str_mv |
Universidade do Minho |
dc.contributor.author.fl_str_mv |
Martins, Albino Duarte, Ana Rita C. Faria, Susana Marques, A. P. Reis, R. L. Neves, N. M. |
dc.subject.por.fl_str_mv |
Drug release Polycaprolactone Mesenchymal stem cells Bone tissue engineering Molecular biology Science & Technology |
topic |
Drug release Polycaprolactone Mesenchymal stem cells Bone tissue engineering Molecular biology Science & Technology |
description |
Electrospun structures were proposed as scaffolds owing to their morphological and structural similarities with the extracellular matrix found in many native tissues. These !brous structures were also proposed as drug release systems by exploiting the direct dependence of the release rate of a drug on the surface area. An osteogenic differentiation factor, dexamethasone (DEX), was incorporated into electrospun polycaprolactone (PCL) nano!bers at different concentrations (5, 10, 15 and 20 wt.% polymer), in a single-step process. The DEX incorporated into the polymeric carrier is in amorphous state, as determined by DSC, and does not in"uence the typical nano!bers morphology. In vitro drug release studies demonstrated that the dexamethasone release was sustained over a period of 15 days. The bioactivity of the released dexamethasone was assessed by cultivating human bone marrow mesenchymal stem cells (hBMSCs) on 15 wt.% DEX-loaded PCL NFMs, under dexamethasone-absent osteogenic differentiation medium formulation. An increased concentration of alkaline phosphatase and deposition of a mineralized matrix was observed. Phenotypic and genotypic expression of osteoblastic-speci!c markers corroborates the osteogenic activity of the loaded growth/differentiation factor. Overall data suggests that the electrospun biodegradable nano!bers can be used as carriers for the sustained release of growth/differentiation factors relevant for bone tissue engineering strategies. |
publishDate |
2010 |
dc.date.none.fl_str_mv |
2010 2010-01-01T00:00:00Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://hdl.handle.net/1822/20565 |
url |
http://hdl.handle.net/1822/20565 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
0142-9612 10.1016/j.biomaterials.2010.04.010 20452016 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.publisher.none.fl_str_mv |
Elsevier |
publisher.none.fl_str_mv |
Elsevier |
dc.source.none.fl_str_mv |
reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação instacron:RCAAP |
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Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
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RCAAP |
institution |
RCAAP |
reponame_str |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
collection |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
repository.name.fl_str_mv |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
repository.mail.fl_str_mv |
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1799133023264833536 |