Modulating cell adhesion to polybutylene succinate biotextile constructs for tissue engineering applications
Autor(a) principal: | |
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Data de Publicação: | 2017 |
Outros Autores: | , , , , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
Texto Completo: | http://hdl.handle.net/1822/42495 |
Resumo: | Textile-based technologies are powerful routes for the production of three-dimensional porous architectures for tissue engineer- ing applications because of their feasibility and possibility for scaling-up. Herein, the use of knitting technology to produce polybutylene succinate fibre-based porous architectures is described. Furthermore, different treatments have been applied to functionalize the surface of the scaffolds developed: sodium hydroxide etching, ultraviolet radiation exposure in an ozone atmosphere and grafting (acrylic acid, vinyl phosphonic acid and vinyl sulphonic acid) after oxygen plasma activation as a way to tailor cell adhesion. A possible effect of the applied treatments on the bulk properties of the textile scaffolds has been considered and thus tensile tests in dry and hydrated states were also carried out. The microscopy results indicated that the surface morphol- ogy and roughness were affected by the applied treatments. The X-ray photoelectron spectroscopy and contact angle measure- ments showed the incorporation of oxygen-containing groups and higher surface free energy as result of the surface treatments applied. The DNA quantification and scanning electron microscopy analysis revealed that these modifications enhanced cell adhesion and altered cell morphology. Generally, sodium hydroxide treatment altered most significantly the surface properties, which in turn resulted in a high number of cells adherent to these surfaces. Based on the results obtained, the proposed surface treatments are appropriate to modify polybutylene succinate knitting scaffolds, influencing cell adhesion and its potential for use in tissue engineering applications. |
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Modulating cell adhesion to polybutylene succinate biotextile constructs for tissue engineering applicationsBiomedicalBiotextileknitted structurePolybutylene succinateScaffoldSurface modificationTissue engineeringScience & TechnologyTextile-based technologies are powerful routes for the production of three-dimensional porous architectures for tissue engineer- ing applications because of their feasibility and possibility for scaling-up. Herein, the use of knitting technology to produce polybutylene succinate fibre-based porous architectures is described. Furthermore, different treatments have been applied to functionalize the surface of the scaffolds developed: sodium hydroxide etching, ultraviolet radiation exposure in an ozone atmosphere and grafting (acrylic acid, vinyl phosphonic acid and vinyl sulphonic acid) after oxygen plasma activation as a way to tailor cell adhesion. A possible effect of the applied treatments on the bulk properties of the textile scaffolds has been considered and thus tensile tests in dry and hydrated states were also carried out. The microscopy results indicated that the surface morphol- ogy and roughness were affected by the applied treatments. The X-ray photoelectron spectroscopy and contact angle measure- ments showed the incorporation of oxygen-containing groups and higher surface free energy as result of the surface treatments applied. The DNA quantification and scanning electron microscopy analysis revealed that these modifications enhanced cell adhesion and altered cell morphology. Generally, sodium hydroxide treatment altered most significantly the surface properties, which in turn resulted in a high number of cells adherent to these surfaces. Based on the results obtained, the proposed surface treatments are appropriate to modify polybutylene succinate knitting scaffolds, influencing cell adhesion and its potential for use in tissue engineering applications. Portuguese Foundation for Science and Technology (FCT) under POCTI and/or FEDERprogrammes under the scope of the project TISSUE2TISSUE (PTDC/CTM/105703/ 2008). The FCT distinction attributed to ALO under the Investigator FCT programme (IF/00411/2013)John Wiley and SonsUniversidade do MinhoRibeiro, Viviana PintoAlmeida, Lília Rosa Santos PintoMartins, Ana Regina FerreiraPashkuleva, I.Marques, A. P.Ribeiro, Ana S.Silva, Carla J.Bonifácio, GraçaSousa, Rui Pedro Romero AmandiOliveira, Ana Leite Almeida MonteiroReis, R. L.20172017-01-01T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/1822/42495engRibeiro V. P., Almeida L., Martins A. R., Pashkuleva I., Marques A. P., Ribeiro A. S., Silva C. J., Bonifácio G., Sousa R. A., Oliveira A. L., Reis R. L. Modulating cell adhesion to polybutylene succinate biotextile constructs for tissue engineering applications, J Tissue Eng Regen Med, doi:10.1002/term.2189, 2017.1932-700510.1002/term.218927412323info:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2023-07-21T12:25:23ZPortal AgregadorONG |
dc.title.none.fl_str_mv |
Modulating cell adhesion to polybutylene succinate biotextile constructs for tissue engineering applications |
title |
Modulating cell adhesion to polybutylene succinate biotextile constructs for tissue engineering applications |
spellingShingle |
Modulating cell adhesion to polybutylene succinate biotextile constructs for tissue engineering applications Ribeiro, Viviana Pinto Biomedical Biotextile knitted structure Polybutylene succinate Scaffold Surface modification Tissue engineering Science & Technology |
title_short |
Modulating cell adhesion to polybutylene succinate biotextile constructs for tissue engineering applications |
title_full |
Modulating cell adhesion to polybutylene succinate biotextile constructs for tissue engineering applications |
title_fullStr |
Modulating cell adhesion to polybutylene succinate biotextile constructs for tissue engineering applications |
title_full_unstemmed |
Modulating cell adhesion to polybutylene succinate biotextile constructs for tissue engineering applications |
title_sort |
Modulating cell adhesion to polybutylene succinate biotextile constructs for tissue engineering applications |
author |
Ribeiro, Viviana Pinto |
author_facet |
Ribeiro, Viviana Pinto Almeida, Lília Rosa Santos Pinto Martins, Ana Regina Ferreira Pashkuleva, I. Marques, A. P. Ribeiro, Ana S. Silva, Carla J. Bonifácio, Graça Sousa, Rui Pedro Romero Amandi Oliveira, Ana Leite Almeida Monteiro Reis, R. L. |
author_role |
author |
author2 |
Almeida, Lília Rosa Santos Pinto Martins, Ana Regina Ferreira Pashkuleva, I. Marques, A. P. Ribeiro, Ana S. Silva, Carla J. Bonifácio, Graça Sousa, Rui Pedro Romero Amandi Oliveira, Ana Leite Almeida Monteiro Reis, R. L. |
author2_role |
author author author author author author author author author author |
dc.contributor.none.fl_str_mv |
Universidade do Minho |
dc.contributor.author.fl_str_mv |
Ribeiro, Viviana Pinto Almeida, Lília Rosa Santos Pinto Martins, Ana Regina Ferreira Pashkuleva, I. Marques, A. P. Ribeiro, Ana S. Silva, Carla J. Bonifácio, Graça Sousa, Rui Pedro Romero Amandi Oliveira, Ana Leite Almeida Monteiro Reis, R. L. |
dc.subject.por.fl_str_mv |
Biomedical Biotextile knitted structure Polybutylene succinate Scaffold Surface modification Tissue engineering Science & Technology |
topic |
Biomedical Biotextile knitted structure Polybutylene succinate Scaffold Surface modification Tissue engineering Science & Technology |
description |
Textile-based technologies are powerful routes for the production of three-dimensional porous architectures for tissue engineer- ing applications because of their feasibility and possibility for scaling-up. Herein, the use of knitting technology to produce polybutylene succinate fibre-based porous architectures is described. Furthermore, different treatments have been applied to functionalize the surface of the scaffolds developed: sodium hydroxide etching, ultraviolet radiation exposure in an ozone atmosphere and grafting (acrylic acid, vinyl phosphonic acid and vinyl sulphonic acid) after oxygen plasma activation as a way to tailor cell adhesion. A possible effect of the applied treatments on the bulk properties of the textile scaffolds has been considered and thus tensile tests in dry and hydrated states were also carried out. The microscopy results indicated that the surface morphol- ogy and roughness were affected by the applied treatments. The X-ray photoelectron spectroscopy and contact angle measure- ments showed the incorporation of oxygen-containing groups and higher surface free energy as result of the surface treatments applied. The DNA quantification and scanning electron microscopy analysis revealed that these modifications enhanced cell adhesion and altered cell morphology. Generally, sodium hydroxide treatment altered most significantly the surface properties, which in turn resulted in a high number of cells adherent to these surfaces. Based on the results obtained, the proposed surface treatments are appropriate to modify polybutylene succinate knitting scaffolds, influencing cell adhesion and its potential for use in tissue engineering applications. |
publishDate |
2017 |
dc.date.none.fl_str_mv |
2017 2017-01-01T00:00:00Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://hdl.handle.net/1822/42495 |
url |
http://hdl.handle.net/1822/42495 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Ribeiro V. P., Almeida L., Martins A. R., Pashkuleva I., Marques A. P., Ribeiro A. S., Silva C. J., Bonifácio G., Sousa R. A., Oliveira A. L., Reis R. L. Modulating cell adhesion to polybutylene succinate biotextile constructs for tissue engineering applications, J Tissue Eng Regen Med, doi:10.1002/term.2189, 2017. 1932-7005 10.1002/term.2189 27412323 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.publisher.none.fl_str_mv |
John Wiley and Sons |
publisher.none.fl_str_mv |
John Wiley and Sons |
dc.source.none.fl_str_mv |
reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação instacron:RCAAP |
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Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
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RCAAP |
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RCAAP |
reponame_str |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
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Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
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