Exosome-Containing Extracellular Vesicles Contribute to the Transport of Resveratrol Metabolites in the Bloodstream

Detalhes bibliográficos
Autor(a) principal: Iglesias-Aguirre, Carlos Eduardo
Data de Publicação: 2022
Outros Autores: Ávila-Gálvez, María Ángeles, López de Las Hazas, María Carmen, Dávalos, Alberto, Espín, Juan Carlos
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
Texto Completo: http://hdl.handle.net/10362/143870
Resumo: Funding: This research was supported by the Projects PID2019-103914RB-I00 and PID2019-109369RBI00 from the Ministry of Science and Innovation (MCIN/AEI/10.13039/501100011033, Spain). C.E.I.- A. is the holder of a predoctoral grant FPU18/03961 from MICIN. M.C.L.-H. is a receipt of a Juan de la Cierva Grant IJC2020-044353-I/MCIN/AEI/10.13039/501100011033/EU/PRTR.
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spelling Exosome-Containing Extracellular Vesicles Contribute to the Transport of Resveratrol Metabolites in the BloodstreamA Human Pharmacokinetic Studydihydroresveratrolexosomeextracellular vesiclesgut microbiotalunularinmetabolitespharmacokineticspolyphenolresveratrolFood ScienceNutrition and DieteticsSDG 3 - Good Health and Well-beingFunding: This research was supported by the Projects PID2019-103914RB-I00 and PID2019-109369RBI00 from the Ministry of Science and Innovation (MCIN/AEI/10.13039/501100011033, Spain). C.E.I.- A. is the holder of a predoctoral grant FPU18/03961 from MICIN. M.C.L.-H. is a receipt of a Juan de la Cierva Grant IJC2020-044353-I/MCIN/AEI/10.13039/501100011033/EU/PRTR.Exosomes are extracellular vesicles (EVs) that regulate intercellular signaling by transferring small RNAs, proteins, nucleic acids, lipids, and other metabolites to local or distant organs, including the brain, by crossing the blood-brain barrier. However, the transport of (poly)phenols in human EVs has not yet been described. Therefore, we aimed here to explore (i) whether resveratrol and (or) its derived metabolites are found in the cargo of human plasma exosome-containing EVs (E-EVs), (ii) when this incorporation occurs, and (iii) whether resveratrol intake stimulates the release of E-EVs. Thus, in a pharmacokinetic study, healthy volunteers (n = 16) consumed 1 capsule (420 mg resveratrol) in the evening before attending the clinic and one more capsule on the day of the pharmacokinetics. The plasma and the isolated E-EVs were analyzed using UPLC-ESI-QTOF-MS. Of 17 metabolites in the plasma, 9 were identified in the E-EVs, but not free resveratrol. The kinetic profiles of resveratrol metabolites were similar in the plasma and the E-EVs, a higher metabolite concentration being detected in the plasma than in the E-EVs. However, the plasma/E-EVs ratio decreased in the gut microbial metabolites, suggesting their better encapsulation efficiency in E-EVs. In addition, glucuronide conjugates of resveratrol, dihydroresveratrol, and lunularin were incorporated into the E-EVs more efficiently than their corresponding sulfates despite glucuronides reaching lower plasma concentrations. Notably, more E-EVs were detected 10 h after resveratrol consumption. This exploratory study provides the first evidence that (i) resveratrol metabolites are transported by E-EVs, with a preference for glucuronide vs. sulfates, (ii) the gut microbial metabolites concentration and kinetic profiles are closely similar in E-EVs and plasma, and (iii) resveratrol intake elicits E-EVs secretion. Overall, these results open new research avenues on the possible role of E-EVs in (poly)phenol health effects.NOVA Medical School|Faculdade de Ciências Médicas (NMS|FCM)RUNIglesias-Aguirre, Carlos EduardoÁvila-Gálvez, María ÁngelesLópez de Las Hazas, María CarmenDávalos, AlbertoEspín, Juan Carlos2022-09-20T22:26:15Z2022-09-022022-09-02T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/10362/143870eng1422-8599PURE: 46652210https://doi.org/10.3390/nu14173632info:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2024-03-11T05:22:43Zoai:run.unl.pt:10362/143870Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-20T03:51:11.147763Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse
dc.title.none.fl_str_mv Exosome-Containing Extracellular Vesicles Contribute to the Transport of Resveratrol Metabolites in the Bloodstream
A Human Pharmacokinetic Study
title Exosome-Containing Extracellular Vesicles Contribute to the Transport of Resveratrol Metabolites in the Bloodstream
spellingShingle Exosome-Containing Extracellular Vesicles Contribute to the Transport of Resveratrol Metabolites in the Bloodstream
Iglesias-Aguirre, Carlos Eduardo
dihydroresveratrol
exosome
extracellular vesicles
gut microbiota
lunularin
metabolites
pharmacokinetics
polyphenol
resveratrol
Food Science
Nutrition and Dietetics
SDG 3 - Good Health and Well-being
title_short Exosome-Containing Extracellular Vesicles Contribute to the Transport of Resveratrol Metabolites in the Bloodstream
title_full Exosome-Containing Extracellular Vesicles Contribute to the Transport of Resveratrol Metabolites in the Bloodstream
title_fullStr Exosome-Containing Extracellular Vesicles Contribute to the Transport of Resveratrol Metabolites in the Bloodstream
title_full_unstemmed Exosome-Containing Extracellular Vesicles Contribute to the Transport of Resveratrol Metabolites in the Bloodstream
title_sort Exosome-Containing Extracellular Vesicles Contribute to the Transport of Resveratrol Metabolites in the Bloodstream
author Iglesias-Aguirre, Carlos Eduardo
author_facet Iglesias-Aguirre, Carlos Eduardo
Ávila-Gálvez, María Ángeles
López de Las Hazas, María Carmen
Dávalos, Alberto
Espín, Juan Carlos
author_role author
author2 Ávila-Gálvez, María Ángeles
López de Las Hazas, María Carmen
Dávalos, Alberto
Espín, Juan Carlos
author2_role author
author
author
author
dc.contributor.none.fl_str_mv NOVA Medical School|Faculdade de Ciências Médicas (NMS|FCM)
RUN
dc.contributor.author.fl_str_mv Iglesias-Aguirre, Carlos Eduardo
Ávila-Gálvez, María Ángeles
López de Las Hazas, María Carmen
Dávalos, Alberto
Espín, Juan Carlos
dc.subject.por.fl_str_mv dihydroresveratrol
exosome
extracellular vesicles
gut microbiota
lunularin
metabolites
pharmacokinetics
polyphenol
resveratrol
Food Science
Nutrition and Dietetics
SDG 3 - Good Health and Well-being
topic dihydroresveratrol
exosome
extracellular vesicles
gut microbiota
lunularin
metabolites
pharmacokinetics
polyphenol
resveratrol
Food Science
Nutrition and Dietetics
SDG 3 - Good Health and Well-being
description Funding: This research was supported by the Projects PID2019-103914RB-I00 and PID2019-109369RBI00 from the Ministry of Science and Innovation (MCIN/AEI/10.13039/501100011033, Spain). C.E.I.- A. is the holder of a predoctoral grant FPU18/03961 from MICIN. M.C.L.-H. is a receipt of a Juan de la Cierva Grant IJC2020-044353-I/MCIN/AEI/10.13039/501100011033/EU/PRTR.
publishDate 2022
dc.date.none.fl_str_mv 2022-09-20T22:26:15Z
2022-09-02
2022-09-02T00:00:00Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://hdl.handle.net/10362/143870
url http://hdl.handle.net/10362/143870
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv 1422-8599
PURE: 46652210
https://doi.org/10.3390/nu14173632
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.source.none.fl_str_mv reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
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instacron:RCAAP
instname_str Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
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reponame_str Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
collection Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
repository.name.fl_str_mv Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
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