Isothermal DNA amplification coupled to Au-nanoprobes for detection of mutations associated to Rifampicin resistance in Mycobacterium tuberculosis

Detalhes bibliográficos
Autor(a) principal: Veigas, Bruno
Data de Publicação: 2013
Outros Autores: Pedrosa, Pedro, Couto, Isabel, Viveiros, Miguel, Baptista, Pedro V.
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
Texto Completo: http://hdl.handle.net/10362/100686
Resumo: We acknowledge Fundacao para a Ciencia e a Tecnologia (FCT-MCTES) for financial support CIGMH (PEst-OE/SAU/UI0009/2011); Projects PTDC/BBB-NAN/1812/2012, PTDC/CTM-NAN/109877/2009; SFRH/BD/78970/2011 for BV. PVB thanks Santander-Totta/UNL for financial support (Scientific Prize Edition 2012).
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spelling Isothermal DNA amplification coupled to Au-nanoprobes for detection of mutations associated to Rifampicin resistance in Mycobacterium tuberculosisDNA isothermal amplificationGold nanoparticlesLAMPMDRTBNanodiagnosticsPCRRifampicinTuberculosisBioengineeringMedicine (miscellaneous)Molecular MedicineBiomedical EngineeringApplied Microbiology and BiotechnologyPharmaceutical ScienceSDG 3 - Good Health and Well-beingWe acknowledge Fundacao para a Ciencia e a Tecnologia (FCT-MCTES) for financial support CIGMH (PEst-OE/SAU/UI0009/2011); Projects PTDC/BBB-NAN/1812/2012, PTDC/CTM-NAN/109877/2009; SFRH/BD/78970/2011 for BV. PVB thanks Santander-Totta/UNL for financial support (Scientific Prize Edition 2012).Background: Tuberculosis accounted for 8.7 million new cases in 2011 and continues to be one of the leading human infectious diseases. Burdensome is the increasing rate of multi-drug resistant tuberculosis (MDRTB) and the difficulties created for treatment and public health control programs, especially in developing countries. Resistance to rifampicin (RIF), a first line antibiotic, is commonly associated with point mutations within the rpoB gene of Mycobacterium tuberculosis (Mtb) whose detection is considered the best early molecular predictor for MDRTB. Gold nanoparticles functionalized with thiol-modified oligonucleotides (Au-nanoprobes) have shown the potential to provide a rapid and sensitive detection method for Mtb and single base alterations associated with antibiotic resistance, namely in rpoB gene associated to RIF resistance.Results: We developed a strategy based on the isothermal amplification of sample DNA (LAMP) coupled to specific Au-nanoprobes capable of identifying members of the Mtb complex (MTBC) and discriminating specific mutations within the rpoB gene. Integration of LAMP and Au-nanoprobe assay allowed to detect MTBC member and identify mutations linked to RIF resistance. A total of 12 biological samples were tested and a 100% specificity and sensitivity was attained.Conclusions: There is an increasing demand for simple, fast and cheap methods for the molecular identification of Mtb and for the detection of molecular tags associated to drug resistance suitable for use at point-of-need. Here we describe such a method, that as the potential to get molecular diagnostic of tuberculosis to remote environments.CENIMAT-i3N - Centro de Investigação de Materiais (Lab. Associado I3N)DCM - Departamento de Ciência dos MateriaisDCV - Departamento de Ciências da VidaCentro de Investigação em Genética Molecular Humana (CIGMH)Instituto de Higiene e Medicina Tropical (IHMT)CREM - Centro de Recursos MicrobiológicosCentro de Malária e outras Doenças Tropicais (CMDT)RUNVeigas, BrunoPedrosa, PedroCouto, IsabelViveiros, MiguelBaptista, Pedro V.2020-07-10T22:22:22Z2013-11-252013-11-25T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/10362/100686eng1477-3155PURE: 18963659https://doi.org/10.1186/1477-3155-11-38info:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2024-03-11T04:47:04Zoai:run.unl.pt:10362/100686Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-20T03:39:24.790404Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse
dc.title.none.fl_str_mv Isothermal DNA amplification coupled to Au-nanoprobes for detection of mutations associated to Rifampicin resistance in Mycobacterium tuberculosis
title Isothermal DNA amplification coupled to Au-nanoprobes for detection of mutations associated to Rifampicin resistance in Mycobacterium tuberculosis
spellingShingle Isothermal DNA amplification coupled to Au-nanoprobes for detection of mutations associated to Rifampicin resistance in Mycobacterium tuberculosis
Veigas, Bruno
DNA isothermal amplification
Gold nanoparticles
LAMP
MDRTB
Nanodiagnostics
PCR
Rifampicin
Tuberculosis
Bioengineering
Medicine (miscellaneous)
Molecular Medicine
Biomedical Engineering
Applied Microbiology and Biotechnology
Pharmaceutical Science
SDG 3 - Good Health and Well-being
title_short Isothermal DNA amplification coupled to Au-nanoprobes for detection of mutations associated to Rifampicin resistance in Mycobacterium tuberculosis
title_full Isothermal DNA amplification coupled to Au-nanoprobes for detection of mutations associated to Rifampicin resistance in Mycobacterium tuberculosis
title_fullStr Isothermal DNA amplification coupled to Au-nanoprobes for detection of mutations associated to Rifampicin resistance in Mycobacterium tuberculosis
title_full_unstemmed Isothermal DNA amplification coupled to Au-nanoprobes for detection of mutations associated to Rifampicin resistance in Mycobacterium tuberculosis
title_sort Isothermal DNA amplification coupled to Au-nanoprobes for detection of mutations associated to Rifampicin resistance in Mycobacterium tuberculosis
author Veigas, Bruno
author_facet Veigas, Bruno
Pedrosa, Pedro
Couto, Isabel
Viveiros, Miguel
Baptista, Pedro V.
author_role author
author2 Pedrosa, Pedro
Couto, Isabel
Viveiros, Miguel
Baptista, Pedro V.
author2_role author
author
author
author
dc.contributor.none.fl_str_mv CENIMAT-i3N - Centro de Investigação de Materiais (Lab. Associado I3N)
DCM - Departamento de Ciência dos Materiais
DCV - Departamento de Ciências da Vida
Centro de Investigação em Genética Molecular Humana (CIGMH)
Instituto de Higiene e Medicina Tropical (IHMT)
CREM - Centro de Recursos Microbiológicos
Centro de Malária e outras Doenças Tropicais (CMDT)
RUN
dc.contributor.author.fl_str_mv Veigas, Bruno
Pedrosa, Pedro
Couto, Isabel
Viveiros, Miguel
Baptista, Pedro V.
dc.subject.por.fl_str_mv DNA isothermal amplification
Gold nanoparticles
LAMP
MDRTB
Nanodiagnostics
PCR
Rifampicin
Tuberculosis
Bioengineering
Medicine (miscellaneous)
Molecular Medicine
Biomedical Engineering
Applied Microbiology and Biotechnology
Pharmaceutical Science
SDG 3 - Good Health and Well-being
topic DNA isothermal amplification
Gold nanoparticles
LAMP
MDRTB
Nanodiagnostics
PCR
Rifampicin
Tuberculosis
Bioengineering
Medicine (miscellaneous)
Molecular Medicine
Biomedical Engineering
Applied Microbiology and Biotechnology
Pharmaceutical Science
SDG 3 - Good Health and Well-being
description We acknowledge Fundacao para a Ciencia e a Tecnologia (FCT-MCTES) for financial support CIGMH (PEst-OE/SAU/UI0009/2011); Projects PTDC/BBB-NAN/1812/2012, PTDC/CTM-NAN/109877/2009; SFRH/BD/78970/2011 for BV. PVB thanks Santander-Totta/UNL for financial support (Scientific Prize Edition 2012).
publishDate 2013
dc.date.none.fl_str_mv 2013-11-25
2013-11-25T00:00:00Z
2020-07-10T22:22:22Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://hdl.handle.net/10362/100686
url http://hdl.handle.net/10362/100686
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv 1477-3155
PURE: 18963659
https://doi.org/10.1186/1477-3155-11-38
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.source.none.fl_str_mv reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron:RCAAP
instname_str Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron_str RCAAP
institution RCAAP
reponame_str Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
collection Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
repository.name.fl_str_mv Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
repository.mail.fl_str_mv
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