Gold nanoprobes for the detection of mutations associated with antibiotic resistance in Mycobacterium tuberculosis complex

Detalhes bibliográficos
Autor(a) principal: Pedrosa, Pedro Miguel Pinto Gonçalves Gouveia
Data de Publicação: 2013
Tipo de documento: Dissertação
Idioma: eng
Título da fonte: Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
Texto Completo: http://hdl.handle.net/10362/10652
Resumo: Dissertação para obtenção do Grau de Mestre em Genética Molecular e Biomedicina
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spelling Gold nanoprobes for the detection of mutations associated with antibiotic resistance in Mycobacterium tuberculosis complexNanodiagnosticsGold nanoparticlesTuberculosisMultiplex PCRDissertação para obtenção do Grau de Mestre em Genética Molecular e BiomedicinaTuberculosis is still one of the leading human infectious diseases, with 8.7 million new cases reported in 2011 alone. Also, the increasing rate of multidrug-resistant tuberculosis (MDR-TB) and its treatment difficulties pose a serious public health threat especially in developing countries. Resistance to isoniazid and rifampicin, first line antibiotics, is commonly associated with point mutations in katG, inhA and rpoB genes of Mycobacterium tuberculosis complex (MTBC). Therefore, the development of a cheap, fast and simple molecular method to assess susceptibility profiles would have a huge impact in the capacity of early diagnosis and treatment of MDR-TB patients. Gold nanoparticles functionalised with thiol-modified oligonucleotides (Au-nanoprobes) have shown the potential to provide a rapid and sensitive detection method for MTBC and single base mutations associated with antibiotic resistance, namely the characterisation of the three most relevant codons in rpoB gene associated to rifampicin resistance. In this work the Au-nanoprobe approach is extended towards the discrimination of specific mutations within inhA gene which is associated with resistance to isoniazid. Using a multiplex PCR reaction for rpoB and inhA genes, it was possible to assess both loci in parallel, and extend the potential of the Au-nanoprobe method to MDR-TB molecular characterisation with special application in the most frequent genotypes circulating in the Lisbon Health Region.Faculdade de Ciências e TecnologiaBaptista, PedroRUNPedrosa, Pedro Miguel Pinto Gonçalves Gouveia2013-10-30T11:14:45Z20132013-01-01T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/masterThesisapplication/pdfhttp://hdl.handle.net/10362/10652enginfo:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2024-03-11T03:44:32Zoai:run.unl.pt:10362/10652Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-20T03:19:38.889688Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse
dc.title.none.fl_str_mv Gold nanoprobes for the detection of mutations associated with antibiotic resistance in Mycobacterium tuberculosis complex
title Gold nanoprobes for the detection of mutations associated with antibiotic resistance in Mycobacterium tuberculosis complex
spellingShingle Gold nanoprobes for the detection of mutations associated with antibiotic resistance in Mycobacterium tuberculosis complex
Pedrosa, Pedro Miguel Pinto Gonçalves Gouveia
Nanodiagnostics
Gold nanoparticles
Tuberculosis
Multiplex PCR
title_short Gold nanoprobes for the detection of mutations associated with antibiotic resistance in Mycobacterium tuberculosis complex
title_full Gold nanoprobes for the detection of mutations associated with antibiotic resistance in Mycobacterium tuberculosis complex
title_fullStr Gold nanoprobes for the detection of mutations associated with antibiotic resistance in Mycobacterium tuberculosis complex
title_full_unstemmed Gold nanoprobes for the detection of mutations associated with antibiotic resistance in Mycobacterium tuberculosis complex
title_sort Gold nanoprobes for the detection of mutations associated with antibiotic resistance in Mycobacterium tuberculosis complex
author Pedrosa, Pedro Miguel Pinto Gonçalves Gouveia
author_facet Pedrosa, Pedro Miguel Pinto Gonçalves Gouveia
author_role author
dc.contributor.none.fl_str_mv Baptista, Pedro
RUN
dc.contributor.author.fl_str_mv Pedrosa, Pedro Miguel Pinto Gonçalves Gouveia
dc.subject.por.fl_str_mv Nanodiagnostics
Gold nanoparticles
Tuberculosis
Multiplex PCR
topic Nanodiagnostics
Gold nanoparticles
Tuberculosis
Multiplex PCR
description Dissertação para obtenção do Grau de Mestre em Genética Molecular e Biomedicina
publishDate 2013
dc.date.none.fl_str_mv 2013-10-30T11:14:45Z
2013
2013-01-01T00:00:00Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/masterThesis
format masterThesis
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://hdl.handle.net/10362/10652
url http://hdl.handle.net/10362/10652
dc.language.iso.fl_str_mv eng
language eng
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Faculdade de Ciências e Tecnologia
publisher.none.fl_str_mv Faculdade de Ciências e Tecnologia
dc.source.none.fl_str_mv reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron:RCAAP
instname_str Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron_str RCAAP
institution RCAAP
reponame_str Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
collection Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
repository.name.fl_str_mv Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
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