Isothermal DNA amplification coupled to Au-nanoprobes for detection of mutations associated to Rifampicin resistance in Mycobacterium tuberculosis
Autor(a) principal: | |
---|---|
Data de Publicação: | 2013 |
Outros Autores: | , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
Texto Completo: | http://hdl.handle.net/10362/100686 |
Resumo: | We acknowledge Fundacao para a Ciencia e a Tecnologia (FCT-MCTES) for financial support CIGMH (PEst-OE/SAU/UI0009/2011); Projects PTDC/BBB-NAN/1812/2012, PTDC/CTM-NAN/109877/2009; SFRH/BD/78970/2011 for BV. PVB thanks Santander-Totta/UNL for financial support (Scientific Prize Edition 2012). |
id |
RCAP_304f4b8dbac21114e91c00906677341c |
---|---|
oai_identifier_str |
oai:run.unl.pt:10362/100686 |
network_acronym_str |
RCAP |
network_name_str |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
repository_id_str |
7160 |
spelling |
Isothermal DNA amplification coupled to Au-nanoprobes for detection of mutations associated to Rifampicin resistance in Mycobacterium tuberculosisDNA isothermal amplificationGold nanoparticlesLAMPMDRTBNanodiagnosticsPCRRifampicinTuberculosisBioengineeringMedicine (miscellaneous)Molecular MedicineBiomedical EngineeringApplied Microbiology and BiotechnologyPharmaceutical ScienceSDG 3 - Good Health and Well-beingWe acknowledge Fundacao para a Ciencia e a Tecnologia (FCT-MCTES) for financial support CIGMH (PEst-OE/SAU/UI0009/2011); Projects PTDC/BBB-NAN/1812/2012, PTDC/CTM-NAN/109877/2009; SFRH/BD/78970/2011 for BV. PVB thanks Santander-Totta/UNL for financial support (Scientific Prize Edition 2012).Background: Tuberculosis accounted for 8.7 million new cases in 2011 and continues to be one of the leading human infectious diseases. Burdensome is the increasing rate of multi-drug resistant tuberculosis (MDRTB) and the difficulties created for treatment and public health control programs, especially in developing countries. Resistance to rifampicin (RIF), a first line antibiotic, is commonly associated with point mutations within the rpoB gene of Mycobacterium tuberculosis (Mtb) whose detection is considered the best early molecular predictor for MDRTB. Gold nanoparticles functionalized with thiol-modified oligonucleotides (Au-nanoprobes) have shown the potential to provide a rapid and sensitive detection method for Mtb and single base alterations associated with antibiotic resistance, namely in rpoB gene associated to RIF resistance.Results: We developed a strategy based on the isothermal amplification of sample DNA (LAMP) coupled to specific Au-nanoprobes capable of identifying members of the Mtb complex (MTBC) and discriminating specific mutations within the rpoB gene. Integration of LAMP and Au-nanoprobe assay allowed to detect MTBC member and identify mutations linked to RIF resistance. A total of 12 biological samples were tested and a 100% specificity and sensitivity was attained.Conclusions: There is an increasing demand for simple, fast and cheap methods for the molecular identification of Mtb and for the detection of molecular tags associated to drug resistance suitable for use at point-of-need. Here we describe such a method, that as the potential to get molecular diagnostic of tuberculosis to remote environments.CENIMAT-i3N - Centro de Investigação de Materiais (Lab. Associado I3N)DCM - Departamento de Ciência dos MateriaisDCV - Departamento de Ciências da VidaCentro de Investigação em Genética Molecular Humana (CIGMH)Instituto de Higiene e Medicina Tropical (IHMT)CREM - Centro de Recursos MicrobiológicosCentro de Malária e outras Doenças Tropicais (CMDT)RUNVeigas, BrunoPedrosa, PedroCouto, IsabelViveiros, MiguelBaptista, Pedro V.2020-07-10T22:22:22Z2013-11-252013-11-25T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/10362/100686eng1477-3155PURE: 18963659https://doi.org/10.1186/1477-3155-11-38info:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2024-03-11T04:47:04Zoai:run.unl.pt:10362/100686Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-20T03:39:24.790404Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse |
dc.title.none.fl_str_mv |
Isothermal DNA amplification coupled to Au-nanoprobes for detection of mutations associated to Rifampicin resistance in Mycobacterium tuberculosis |
title |
Isothermal DNA amplification coupled to Au-nanoprobes for detection of mutations associated to Rifampicin resistance in Mycobacterium tuberculosis |
spellingShingle |
Isothermal DNA amplification coupled to Au-nanoprobes for detection of mutations associated to Rifampicin resistance in Mycobacterium tuberculosis Veigas, Bruno DNA isothermal amplification Gold nanoparticles LAMP MDRTB Nanodiagnostics PCR Rifampicin Tuberculosis Bioengineering Medicine (miscellaneous) Molecular Medicine Biomedical Engineering Applied Microbiology and Biotechnology Pharmaceutical Science SDG 3 - Good Health and Well-being |
title_short |
Isothermal DNA amplification coupled to Au-nanoprobes for detection of mutations associated to Rifampicin resistance in Mycobacterium tuberculosis |
title_full |
Isothermal DNA amplification coupled to Au-nanoprobes for detection of mutations associated to Rifampicin resistance in Mycobacterium tuberculosis |
title_fullStr |
Isothermal DNA amplification coupled to Au-nanoprobes for detection of mutations associated to Rifampicin resistance in Mycobacterium tuberculosis |
title_full_unstemmed |
Isothermal DNA amplification coupled to Au-nanoprobes for detection of mutations associated to Rifampicin resistance in Mycobacterium tuberculosis |
title_sort |
Isothermal DNA amplification coupled to Au-nanoprobes for detection of mutations associated to Rifampicin resistance in Mycobacterium tuberculosis |
author |
Veigas, Bruno |
author_facet |
Veigas, Bruno Pedrosa, Pedro Couto, Isabel Viveiros, Miguel Baptista, Pedro V. |
author_role |
author |
author2 |
Pedrosa, Pedro Couto, Isabel Viveiros, Miguel Baptista, Pedro V. |
author2_role |
author author author author |
dc.contributor.none.fl_str_mv |
CENIMAT-i3N - Centro de Investigação de Materiais (Lab. Associado I3N) DCM - Departamento de Ciência dos Materiais DCV - Departamento de Ciências da Vida Centro de Investigação em Genética Molecular Humana (CIGMH) Instituto de Higiene e Medicina Tropical (IHMT) CREM - Centro de Recursos Microbiológicos Centro de Malária e outras Doenças Tropicais (CMDT) RUN |
dc.contributor.author.fl_str_mv |
Veigas, Bruno Pedrosa, Pedro Couto, Isabel Viveiros, Miguel Baptista, Pedro V. |
dc.subject.por.fl_str_mv |
DNA isothermal amplification Gold nanoparticles LAMP MDRTB Nanodiagnostics PCR Rifampicin Tuberculosis Bioengineering Medicine (miscellaneous) Molecular Medicine Biomedical Engineering Applied Microbiology and Biotechnology Pharmaceutical Science SDG 3 - Good Health and Well-being |
topic |
DNA isothermal amplification Gold nanoparticles LAMP MDRTB Nanodiagnostics PCR Rifampicin Tuberculosis Bioengineering Medicine (miscellaneous) Molecular Medicine Biomedical Engineering Applied Microbiology and Biotechnology Pharmaceutical Science SDG 3 - Good Health and Well-being |
description |
We acknowledge Fundacao para a Ciencia e a Tecnologia (FCT-MCTES) for financial support CIGMH (PEst-OE/SAU/UI0009/2011); Projects PTDC/BBB-NAN/1812/2012, PTDC/CTM-NAN/109877/2009; SFRH/BD/78970/2011 for BV. PVB thanks Santander-Totta/UNL for financial support (Scientific Prize Edition 2012). |
publishDate |
2013 |
dc.date.none.fl_str_mv |
2013-11-25 2013-11-25T00:00:00Z 2020-07-10T22:22:22Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://hdl.handle.net/10362/100686 |
url |
http://hdl.handle.net/10362/100686 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
1477-3155 PURE: 18963659 https://doi.org/10.1186/1477-3155-11-38 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.source.none.fl_str_mv |
reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação instacron:RCAAP |
instname_str |
Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
instacron_str |
RCAAP |
institution |
RCAAP |
reponame_str |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
collection |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
repository.name.fl_str_mv |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
repository.mail.fl_str_mv |
|
_version_ |
1799138009971425280 |