Acetylated cashew gum and fucan for incorporation of lycopene rich extract from red guava (Psidium guajava L.) in nanostructured systems: antioxidant and antitumor capacity

• Autor(a) principal: Andrades, Eryka Oliveira de
• Data de Publicação: 2021
• Outros Autores: Costa, João Marcos Antônio Rodrigues da, Neto, Francisco Edmar Moreira de Lima, Araujo, Alyne Rodrigues de, Ribeiro, Fabio de Oliveira Silva, Vasconcelos, Andreanne Gomes, Oliveira, Antônia Carla de Jesus, Sobrinho, José Lamartine Soares, Almeida, Miguel Peixoto de, Carvalho, Ana P., Dias, Jhones Nascimento, Silva, Ingrid Gracielle Martins, Albuquerque, Patrícia, Pereira, Ildinete Silva, Rabello, Doralina do Amaral, Amorim, Adriany das Graças Nascimento, Leite, José Roberto de Souza de Almeida, Silva, Durcilene Alves da
• Tipo de documento: Artigo
• Idioma: eng
• Título da fonte: Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
• Texto Completo: http://hdl.handle.net/10400.14/35630
• topic: Antioxidant; Antitumor; Biocompatibility; Lycopene; Nanoparticles; Polysaccharides

Industrial application of lycopene is limited due to its chemical instability and low bioavailability. This study proposes the development of fucan-coated acetylated cashew gum nanoparticles (NFGa) and acetylated cashew gum nanoparticles (NGa) for incorporation of the lycopene-rich extract from red guava (LEG). Size, polydispersity, zeta potential, nanoparticles concentration, encapsulation efficiency, transmission electron microscopy (TEM) and atomic force microscopy (AFM) were used to characterize nanoparticles. The antioxidant activity was determinated and cell viability was evaluated in the human breast cancer cells (MCF-7) and human keratinocytes (HaCaT) by MTT assay. The toxic effect was evaluated by hemolysis test and by Galleria mellonella model. NFGa showed higher stability than NGa, having a size of 162.10 ± 3.21 nm, polydispersity of 0.348 ± 0.019, zeta potential -30.70 ± 0.53 mV, concentration of 6.4 × 109 nanoparticles/mL and 60% LEG encapsulation. Microscopic analysis revealed a spherical and smooth shape of NFGa. NFGa showed antioxidant capacity by ABTS method and ORAC assay. The NFGa presented significant cytotoxicity against MCF-7 from the lowest concentration tested (6.25-200 μg/mL) and did not affect the cell viability of the HaCaT. NFGa showed non-toxic effect in the in vitro and in vivo models. Therefore, NFGa may have a promising application in LEG stabilization for antioxidant and antitumor purposes.