Development of chitosan-based microparticles for pulmonary drug delivery

Detalhes bibliográficos
Autor(a) principal: Cabral, Renato Manuel Pereira
Data de Publicação: 2013
Tipo de documento: Dissertação
Idioma: eng
Título da fonte: Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
Texto Completo: http://hdl.handle.net/10362/10505
Resumo: Dissertação para obtenção do Grau de Mestre em Engenharia Química e Bioquímica
id RCAP_3170832e0d58ee3c90a006dbf1ac1c2a
oai_identifier_str oai:run.unl.pt:10362/10505
network_acronym_str RCAP
network_name_str Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
repository_id_str 7160
spelling Development of chitosan-based microparticles for pulmonary drug deliveryMicroparticlesChitosanSupercritical assisted atomizationPulmonary deliveryDry powder formulationsDissertação para obtenção do Grau de Mestre em Engenharia Química e BioquímicaIn this work, novel chitosan (CHT) based microparticles were prepared using supercritical assisted atomization (SAA) and evaluated as potential carriers for sustained pulmonary drug delivery. CHT is a polysaccharide comprising of glucosamine and N-acetylglucosamine units, it is biodegradable, biocompatible and non-toxic being an interesting choice to be used as a drug carrier for inhalation therapy and belongs to the group of swellable polymers. By utilizing SAA, spherical microparticles containing a sharp particle distribution were successfully produced. Ibuprofen (IBP) and bovine serum albumin (BSA) were tested as a model small drug and as a model protein, respectively, to assess the effect on particle size and morphology when co-atomized with CHT on the SAA apparatus. The strategy developed in this work was to produce drug loaded microparticles with suitable aerodynamic characteristics that attain large geometric diameters when in contact with the lung physiological fluids by polymer swelling, reducing macrophage clearance. The microparticles produced by this method were characterized by using Morphologi G3 and Scanning electron microscopy (SEM) to assess their size distribution and morphology. To characterize the solid state properties of the particles X-ray diffraction (XRD), differential scanning calorimetry DSC and Fourier transform infrared (FTIR) were used. Porosity and surface area were determined by mercury and nitrogen porosimetry. In vitro aerosolization studies using an Andersen Cascade Impactor (ACI) were performed to determine the average emitted fraction (EF%) and the fine particle fraction (FPF). Drug-release profiles were determined by in vitro experiments at physiological pH and temperature conditions. The results obtained in this work show that SAA can be successfully used to prepare chitosan based formulations with adequate respirable fractions and sustained release of different bioactive molecules to be administered to the deep lung using dry powder inhalers (DPI).Fundação para a Ciência e Tecnologia - contracts PEst-C/EQB/LA0006/2011, PTDC/EQU-EQU/116097/2009, Conselho de Reitores das Universidades Portuguesas (CRUP) through Luso-German Agreement A - 13/ 10 and from Fundação Calouste Gulbenkian is acknowledgedFaculdade de Ciências e TecnologiaRicardo, AnaRibeiro, TeresaRUNCabral, Renato Manuel Pereira2013-10-07T09:44:36Z20132013-01-01T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/masterThesisapplication/pdfhttp://hdl.handle.net/10362/10505enginfo:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2024-03-11T03:44:17Zoai:run.unl.pt:10362/10505Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-20T03:19:30.383336Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse
dc.title.none.fl_str_mv Development of chitosan-based microparticles for pulmonary drug delivery
title Development of chitosan-based microparticles for pulmonary drug delivery
spellingShingle Development of chitosan-based microparticles for pulmonary drug delivery
Cabral, Renato Manuel Pereira
Microparticles
Chitosan
Supercritical assisted atomization
Pulmonary delivery
Dry powder formulations
title_short Development of chitosan-based microparticles for pulmonary drug delivery
title_full Development of chitosan-based microparticles for pulmonary drug delivery
title_fullStr Development of chitosan-based microparticles for pulmonary drug delivery
title_full_unstemmed Development of chitosan-based microparticles for pulmonary drug delivery
title_sort Development of chitosan-based microparticles for pulmonary drug delivery
author Cabral, Renato Manuel Pereira
author_facet Cabral, Renato Manuel Pereira
author_role author
dc.contributor.none.fl_str_mv Ricardo, Ana
Ribeiro, Teresa
RUN
dc.contributor.author.fl_str_mv Cabral, Renato Manuel Pereira
dc.subject.por.fl_str_mv Microparticles
Chitosan
Supercritical assisted atomization
Pulmonary delivery
Dry powder formulations
topic Microparticles
Chitosan
Supercritical assisted atomization
Pulmonary delivery
Dry powder formulations
description Dissertação para obtenção do Grau de Mestre em Engenharia Química e Bioquímica
publishDate 2013
dc.date.none.fl_str_mv 2013-10-07T09:44:36Z
2013
2013-01-01T00:00:00Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/masterThesis
format masterThesis
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://hdl.handle.net/10362/10505
url http://hdl.handle.net/10362/10505
dc.language.iso.fl_str_mv eng
language eng
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Faculdade de Ciências e Tecnologia
publisher.none.fl_str_mv Faculdade de Ciências e Tecnologia
dc.source.none.fl_str_mv reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron:RCAAP
instname_str Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron_str RCAAP
institution RCAAP
reponame_str Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
collection Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
repository.name.fl_str_mv Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
repository.mail.fl_str_mv
_version_ 1799137838534492160

Registros relacionados