Development and characterization of chitosan microparticles-in-films for buccal delivery of bioactive peptides

Detalhes bibliográficos
Autor(a) principal: Batista, Patrícia
Data de Publicação: 2019
Outros Autores: Castro, Pedro, Madureira, Ana Raquel, Sarmento, Bruno, Pintado, Manuela
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
Texto Completo: http://hdl.handle.net/10400.14/27077
Resumo: Nowadays, bioactive peptides are used for therapeutic applications and the selection of a carrier to deliver them is very important to increase the efficiency, absorption, release, bioavailability and consumer acceptance. The aim of this study was to develop and characterize chitosan-based films loaded with chitosan microparticles containing a bioactive peptide (sequence: KGYGGVSLPEW) with antihypertensive properties. Films were prepared by the solvent casting method, while the microparticles were prepared by ionic gelation. The final optimized chitosan microparticles exhibited a mean diameter of 2.5 m, a polydispersity index of 0.46, a zeta potential of +61 mV and a peptide association efficiency of 76%. Chitosan films were optimized achieving the final formulation of 0.79% (w/v) of chitosan, 6.74% (w/v) of sorbitol and 0.82% (w/v) of citric acid. These thin ( 0.100 mm) and transparent films demonstrated good performance in terms of mechanical and biological properties. The oral films developed were flexible, elastic, easy to handle and exhibited rapid disintegration (30 s) and an erosion behavior of 20% when they came into contact with saliva solution. The cell viability (75–99%) was proved by methylthiazolydiphenyl-tetrazolium bromide (MTT) assay with TR146 cells. The chitosan mucoadhesive films loaded with peptide–chitosan microparticles resulted in an innovative approach to perform administration across the buccal mucosa, because these films present a larger surface area, leading to the rapid disintegration and release of the antihypertensive peptide under controlled conditions in the buccal cavity, thus promoting bioavailability.
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spelling Development and characterization of chitosan microparticles-in-films for buccal delivery of bioactive peptidesBioactive peptidesBuccal deliveryChitosanMicroparticlesOral filmsNowadays, bioactive peptides are used for therapeutic applications and the selection of a carrier to deliver them is very important to increase the efficiency, absorption, release, bioavailability and consumer acceptance. The aim of this study was to develop and characterize chitosan-based films loaded with chitosan microparticles containing a bioactive peptide (sequence: KGYGGVSLPEW) with antihypertensive properties. Films were prepared by the solvent casting method, while the microparticles were prepared by ionic gelation. The final optimized chitosan microparticles exhibited a mean diameter of 2.5 m, a polydispersity index of 0.46, a zeta potential of +61 mV and a peptide association efficiency of 76%. Chitosan films were optimized achieving the final formulation of 0.79% (w/v) of chitosan, 6.74% (w/v) of sorbitol and 0.82% (w/v) of citric acid. These thin ( 0.100 mm) and transparent films demonstrated good performance in terms of mechanical and biological properties. The oral films developed were flexible, elastic, easy to handle and exhibited rapid disintegration (30 s) and an erosion behavior of 20% when they came into contact with saliva solution. The cell viability (75–99%) was proved by methylthiazolydiphenyl-tetrazolium bromide (MTT) assay with TR146 cells. The chitosan mucoadhesive films loaded with peptide–chitosan microparticles resulted in an innovative approach to perform administration across the buccal mucosa, because these films present a larger surface area, leading to the rapid disintegration and release of the antihypertensive peptide under controlled conditions in the buccal cavity, thus promoting bioavailability.MDPIVeritati - Repositório Institucional da Universidade Católica PortuguesaBatista, PatríciaCastro, PedroMadureira, Ana RaquelSarmento, BrunoPintado, Manuela2019-03-12T16:42:47Z20192019-01-01T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/10400.14/27077engBatista, P., Castro, P., Madureira, A., Sarmento, B., & Pintado, M. (2019). Development and Characterization of Chitosan Microparticles-in-Films for Buccal Delivery of Bioactive Peptides. Pharmaceuticals, 12(1), 32. https://doi.org/10.3390/ph120100321424-824710.3390/ph1201003285064350500PMC646917130791572000464181700002info:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2023-10-03T01:41:43Zoai:repositorio.ucp.pt:10400.14/27077Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-19T18:21:39.449148Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse
dc.title.none.fl_str_mv Development and characterization of chitosan microparticles-in-films for buccal delivery of bioactive peptides
title Development and characterization of chitosan microparticles-in-films for buccal delivery of bioactive peptides
spellingShingle Development and characterization of chitosan microparticles-in-films for buccal delivery of bioactive peptides
Batista, Patrícia
Bioactive peptides
Buccal delivery
Chitosan
Microparticles
Oral films
title_short Development and characterization of chitosan microparticles-in-films for buccal delivery of bioactive peptides
title_full Development and characterization of chitosan microparticles-in-films for buccal delivery of bioactive peptides
title_fullStr Development and characterization of chitosan microparticles-in-films for buccal delivery of bioactive peptides
title_full_unstemmed Development and characterization of chitosan microparticles-in-films for buccal delivery of bioactive peptides
title_sort Development and characterization of chitosan microparticles-in-films for buccal delivery of bioactive peptides
author Batista, Patrícia
author_facet Batista, Patrícia
Castro, Pedro
Madureira, Ana Raquel
Sarmento, Bruno
Pintado, Manuela
author_role author
author2 Castro, Pedro
Madureira, Ana Raquel
Sarmento, Bruno
Pintado, Manuela
author2_role author
author
author
author
dc.contributor.none.fl_str_mv Veritati - Repositório Institucional da Universidade Católica Portuguesa
dc.contributor.author.fl_str_mv Batista, Patrícia
Castro, Pedro
Madureira, Ana Raquel
Sarmento, Bruno
Pintado, Manuela
dc.subject.por.fl_str_mv Bioactive peptides
Buccal delivery
Chitosan
Microparticles
Oral films
topic Bioactive peptides
Buccal delivery
Chitosan
Microparticles
Oral films
description Nowadays, bioactive peptides are used for therapeutic applications and the selection of a carrier to deliver them is very important to increase the efficiency, absorption, release, bioavailability and consumer acceptance. The aim of this study was to develop and characterize chitosan-based films loaded with chitosan microparticles containing a bioactive peptide (sequence: KGYGGVSLPEW) with antihypertensive properties. Films were prepared by the solvent casting method, while the microparticles were prepared by ionic gelation. The final optimized chitosan microparticles exhibited a mean diameter of 2.5 m, a polydispersity index of 0.46, a zeta potential of +61 mV and a peptide association efficiency of 76%. Chitosan films were optimized achieving the final formulation of 0.79% (w/v) of chitosan, 6.74% (w/v) of sorbitol and 0.82% (w/v) of citric acid. These thin ( 0.100 mm) and transparent films demonstrated good performance in terms of mechanical and biological properties. The oral films developed were flexible, elastic, easy to handle and exhibited rapid disintegration (30 s) and an erosion behavior of 20% when they came into contact with saliva solution. The cell viability (75–99%) was proved by methylthiazolydiphenyl-tetrazolium bromide (MTT) assay with TR146 cells. The chitosan mucoadhesive films loaded with peptide–chitosan microparticles resulted in an innovative approach to perform administration across the buccal mucosa, because these films present a larger surface area, leading to the rapid disintegration and release of the antihypertensive peptide under controlled conditions in the buccal cavity, thus promoting bioavailability.
publishDate 2019
dc.date.none.fl_str_mv 2019-03-12T16:42:47Z
2019
2019-01-01T00:00:00Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://hdl.handle.net/10400.14/27077
url http://hdl.handle.net/10400.14/27077
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv Batista, P., Castro, P., Madureira, A., Sarmento, B., & Pintado, M. (2019). Development and Characterization of Chitosan Microparticles-in-Films for Buccal Delivery of Bioactive Peptides. Pharmaceuticals, 12(1), 32. https://doi.org/10.3390/ph12010032
1424-8247
10.3390/ph12010032
85064350500
PMC6469171
30791572
000464181700002
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
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instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron:RCAAP
instname_str Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
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reponame_str Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
collection Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
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