Methylobacterium oryzae influences isoepoxydon dehydrogenase gene expression and patulin production by Penicillium expansum
Autor(a) principal: | |
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Data de Publicação: | 2021 |
Outros Autores: | , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
Texto Completo: | http://hdl.handle.net/1822/73435 |
Resumo: | Biofilms can be considered the main source of microorganisms in drinking water distribution systems (DWDS). The ecology of a biofilm is dependent on a variety of factors, including the presence of microbial metabolites excreted by its inhabitants. This study reports the effect of the Gram-negative bacteria Methylobacterium oryzae on the idh gene expression levels and patulin production of Penicillium expansum mature biofilms. For this purpose, a RT-qPCR method to quantify idh mRNA levels was applied. In addition, the idh expression levels were compared with the patulin production. The results obtained revealed that the effect of the bacterium on pre-established P. expansum biofilms is dependent on the time of interaction. More mature P. expansum biofilms appear to be more resistant to the inhibitory effect that M. oryzae causes towards idh gene expression and patulin production. A positive trend was observed between the idh expression and patulin production values. The results indicate that M. oryzae affects patulin production by acting at the transcriptional level of the idh gene. |
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Methylobacterium oryzae influences isoepoxydon dehydrogenase gene expression and patulin production by Penicillium expansumBacteriaFungiInter-kingdom biofilmsIdh gene expressionPatulinQuorum sensing inhibitorScience & TechnologyBiofilms can be considered the main source of microorganisms in drinking water distribution systems (DWDS). The ecology of a biofilm is dependent on a variety of factors, including the presence of microbial metabolites excreted by its inhabitants. This study reports the effect of the Gram-negative bacteria Methylobacterium oryzae on the idh gene expression levels and patulin production of Penicillium expansum mature biofilms. For this purpose, a RT-qPCR method to quantify idh mRNA levels was applied. In addition, the idh expression levels were compared with the patulin production. The results obtained revealed that the effect of the bacterium on pre-established P. expansum biofilms is dependent on the time of interaction. More mature P. expansum biofilms appear to be more resistant to the inhibitory effect that M. oryzae causes towards idh gene expression and patulin production. A positive trend was observed between the idh expression and patulin production values. The results indicate that M. oryzae affects patulin production by acting at the transcriptional level of the idh gene.Tiago B. Afonso acknowledges the grant provided by the Portuguese Foundation for Science and Technology (FCT) under grant no. PD/BD/128033/2016. Furthermore, this study was also supported by FCT under the scope of the strategic funding of UIDB/04469/2020 unit and BioTecNorte operation (NORTE-01-0145-FEDER-000004) funded by the European Regional Development Fund under the scope of Norte2020—Programa Operacional Regional do Norte.info:eu-repo/semantics/publishedVersionMultidisciplinary Digital Publishing Institute (MDPI)Universidade do MinhoAfonso, T. B.Simões, Lúcia ChavesLima, Nelson2021-05-202021-05-20T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/1822/73435engAfonso, T. B.; Simões, Lúcia C.; Lima, Nelson, Methylobacterium oryzae influences isoepoxydon dehydrogenase gene expression and patulin production by Penicillium expansum. Water, 13(10), 1427, 20212073-444110.3390/w13101427https://www.mdpi.com/2073-4441/13/10/1427info:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2023-07-21T12:18:03Zoai:repositorium.sdum.uminho.pt:1822/73435Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-19T19:10:47.324873Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse |
dc.title.none.fl_str_mv |
Methylobacterium oryzae influences isoepoxydon dehydrogenase gene expression and patulin production by Penicillium expansum |
title |
Methylobacterium oryzae influences isoepoxydon dehydrogenase gene expression and patulin production by Penicillium expansum |
spellingShingle |
Methylobacterium oryzae influences isoepoxydon dehydrogenase gene expression and patulin production by Penicillium expansum Afonso, T. B. Bacteria Fungi Inter-kingdom biofilms Idh gene expression Patulin Quorum sensing inhibitor Science & Technology |
title_short |
Methylobacterium oryzae influences isoepoxydon dehydrogenase gene expression and patulin production by Penicillium expansum |
title_full |
Methylobacterium oryzae influences isoepoxydon dehydrogenase gene expression and patulin production by Penicillium expansum |
title_fullStr |
Methylobacterium oryzae influences isoepoxydon dehydrogenase gene expression and patulin production by Penicillium expansum |
title_full_unstemmed |
Methylobacterium oryzae influences isoepoxydon dehydrogenase gene expression and patulin production by Penicillium expansum |
title_sort |
Methylobacterium oryzae influences isoepoxydon dehydrogenase gene expression and patulin production by Penicillium expansum |
author |
Afonso, T. B. |
author_facet |
Afonso, T. B. Simões, Lúcia Chaves Lima, Nelson |
author_role |
author |
author2 |
Simões, Lúcia Chaves Lima, Nelson |
author2_role |
author author |
dc.contributor.none.fl_str_mv |
Universidade do Minho |
dc.contributor.author.fl_str_mv |
Afonso, T. B. Simões, Lúcia Chaves Lima, Nelson |
dc.subject.por.fl_str_mv |
Bacteria Fungi Inter-kingdom biofilms Idh gene expression Patulin Quorum sensing inhibitor Science & Technology |
topic |
Bacteria Fungi Inter-kingdom biofilms Idh gene expression Patulin Quorum sensing inhibitor Science & Technology |
description |
Biofilms can be considered the main source of microorganisms in drinking water distribution systems (DWDS). The ecology of a biofilm is dependent on a variety of factors, including the presence of microbial metabolites excreted by its inhabitants. This study reports the effect of the Gram-negative bacteria Methylobacterium oryzae on the idh gene expression levels and patulin production of Penicillium expansum mature biofilms. For this purpose, a RT-qPCR method to quantify idh mRNA levels was applied. In addition, the idh expression levels were compared with the patulin production. The results obtained revealed that the effect of the bacterium on pre-established P. expansum biofilms is dependent on the time of interaction. More mature P. expansum biofilms appear to be more resistant to the inhibitory effect that M. oryzae causes towards idh gene expression and patulin production. A positive trend was observed between the idh expression and patulin production values. The results indicate that M. oryzae affects patulin production by acting at the transcriptional level of the idh gene. |
publishDate |
2021 |
dc.date.none.fl_str_mv |
2021-05-20 2021-05-20T00:00:00Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://hdl.handle.net/1822/73435 |
url |
http://hdl.handle.net/1822/73435 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Afonso, T. B.; Simões, Lúcia C.; Lima, Nelson, Methylobacterium oryzae influences isoepoxydon dehydrogenase gene expression and patulin production by Penicillium expansum. Water, 13(10), 1427, 2021 2073-4441 10.3390/w13101427 https://www.mdpi.com/2073-4441/13/10/1427 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.publisher.none.fl_str_mv |
Multidisciplinary Digital Publishing Institute (MDPI) |
publisher.none.fl_str_mv |
Multidisciplinary Digital Publishing Institute (MDPI) |
dc.source.none.fl_str_mv |
reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação instacron:RCAAP |
instname_str |
Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
instacron_str |
RCAAP |
institution |
RCAAP |
reponame_str |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
collection |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
repository.name.fl_str_mv |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
repository.mail.fl_str_mv |
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1799132538233421824 |