Primers from the isoepoxydon dehydrogenase gene of the patulin biosynthetic pathway to indicate critical control points for patulin contamination of apples

Detalhes bibliográficos
Autor(a) principal: Paterson, R. R. M.
Data de Publicação: 2006
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
Texto Completo: http://hdl.handle.net/1822/4851
Resumo: The mycotoxin patulin is subjected to European Union statutory limits, and has action levels imposed in the United States of America in fruit products. Samples from soil and an orchard were analysed by primers of the isoepoxydon dehydrogenase gene of the patulin biosynthetic pathway as an HACCP procedure in an initial assessment. A non-orchard soil appeared to inhibit the reaction. However, the reaction was positive from orchard soils. Negative results from orchard samples appeared to be because of target DNA being absent. Bramley samples contained the gene frequently. The method may be useful in the determination of critical control points.
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spelling Primers from the isoepoxydon dehydrogenase gene of the patulin biosynthetic pathway to indicate critical control points for patulin contamination of applesPatulinApplesControlIDH geneScience & TechnologyThe mycotoxin patulin is subjected to European Union statutory limits, and has action levels imposed in the United States of America in fruit products. Samples from soil and an orchard were analysed by primers of the isoepoxydon dehydrogenase gene of the patulin biosynthetic pathway as an HACCP procedure in an initial assessment. A non-orchard soil appeared to inhibit the reaction. However, the reaction was positive from orchard soils. Negative results from orchard samples appeared to be because of target DNA being absent. Bramley samples contained the gene frequently. The method may be useful in the determination of critical control points.Fundação para a Ciência e a Tecnologia (FCT); UK Food Standards Agency; CABI Bioscience.Elsevier Ltd.Universidade do MinhoPaterson, R. R. M.2006-092006-09-01T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/1822/4851eng"Food control". ISSN 0956-7135. 17:9 (Sept. 2006) 741-744.0956-713510.1016/j.foodcont.2005.04.016info:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2023-07-21T12:19:11Zoai:repositorium.sdum.uminho.pt:1822/4851Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-19T19:12:07.025558Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse
dc.title.none.fl_str_mv Primers from the isoepoxydon dehydrogenase gene of the patulin biosynthetic pathway to indicate critical control points for patulin contamination of apples
title Primers from the isoepoxydon dehydrogenase gene of the patulin biosynthetic pathway to indicate critical control points for patulin contamination of apples
spellingShingle Primers from the isoepoxydon dehydrogenase gene of the patulin biosynthetic pathway to indicate critical control points for patulin contamination of apples
Paterson, R. R. M.
Patulin
Apples
Control
IDH gene
Science & Technology
title_short Primers from the isoepoxydon dehydrogenase gene of the patulin biosynthetic pathway to indicate critical control points for patulin contamination of apples
title_full Primers from the isoepoxydon dehydrogenase gene of the patulin biosynthetic pathway to indicate critical control points for patulin contamination of apples
title_fullStr Primers from the isoepoxydon dehydrogenase gene of the patulin biosynthetic pathway to indicate critical control points for patulin contamination of apples
title_full_unstemmed Primers from the isoepoxydon dehydrogenase gene of the patulin biosynthetic pathway to indicate critical control points for patulin contamination of apples
title_sort Primers from the isoepoxydon dehydrogenase gene of the patulin biosynthetic pathway to indicate critical control points for patulin contamination of apples
author Paterson, R. R. M.
author_facet Paterson, R. R. M.
author_role author
dc.contributor.none.fl_str_mv Universidade do Minho
dc.contributor.author.fl_str_mv Paterson, R. R. M.
dc.subject.por.fl_str_mv Patulin
Apples
Control
IDH gene
Science & Technology
topic Patulin
Apples
Control
IDH gene
Science & Technology
description The mycotoxin patulin is subjected to European Union statutory limits, and has action levels imposed in the United States of America in fruit products. Samples from soil and an orchard were analysed by primers of the isoepoxydon dehydrogenase gene of the patulin biosynthetic pathway as an HACCP procedure in an initial assessment. A non-orchard soil appeared to inhibit the reaction. However, the reaction was positive from orchard soils. Negative results from orchard samples appeared to be because of target DNA being absent. Bramley samples contained the gene frequently. The method may be useful in the determination of critical control points.
publishDate 2006
dc.date.none.fl_str_mv 2006-09
2006-09-01T00:00:00Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://hdl.handle.net/1822/4851
url http://hdl.handle.net/1822/4851
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv "Food control". ISSN 0956-7135. 17:9 (Sept. 2006) 741-744.
0956-7135
10.1016/j.foodcont.2005.04.016
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Elsevier Ltd.
publisher.none.fl_str_mv Elsevier Ltd.
dc.source.none.fl_str_mv reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron:RCAAP
instname_str Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron_str RCAAP
institution RCAAP
reponame_str Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
collection Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
repository.name.fl_str_mv Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
repository.mail.fl_str_mv
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