Identification and functional analysis of novel genes expressed in the Anterior Visceral Endoderm
Autor(a) principal: | |
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Data de Publicação: | 2011 |
Outros Autores: | , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
Texto Completo: | http://hdl.handle.net/10400.7/692 |
Resumo: | During early vertebrate development, the correct establishment of the body axes is critical. The anterior pole of the mouse embryo is established when Distal Visceral Endoderm (DVE) cells migrate to form the Anterior Visceral Endoderm (AVE). Symmetrical expression of Lefty1, Cer1 and Dkk1 determines the direction of DVE migration and the future anterior side. In addition to the establishment of the Anterior-Posterior axis, the AVE has also been implicated in anterior neural specification. To better understand the role of the AVE in these processes, we have performed a differential screening using Affymetrix GeneChip technology with AVE cells isolated from cer1P-EGFP transgenic mouse embryos. We found 175 genes which were upregulated in the AVE and 36 genes in the Proximal-posterior sample. Using DAVID software, we characterized the AVE cell population regarding cellular component, molecular function and biological processes. Among the genes that were found to be upregulated in the AVE, several novel genes were identified. Four of these transcripts displaying high-fold change in the AVE were further characterized by in situ hybridization in early stages of development in order to validate the screening. From those four selected genes, one, denominated Adtk1, was chosen to be functionally characterized by targeted inactivation in ES cells. Adtk1 encodes for a serine/threonine kinase. Adtk1 null mutants are smaller and present short limbs due to decreased mineralization, suggesting a potential role in chondrogenesis during limb development. Taken together, these data point to the importance of reporting novel genes present in the AVE. |
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Identification and functional analysis of novel genes expressed in the Anterior Visceral EndodermAmino Acid SequenceAnimalsCell MovementChondrogenesisCytokinesEmbryo, MammalianIntercellular Signaling Peptides and ProteinsLeft-Right Determination FactorsMiceMice, Inbred C57BLMice, TransgenicProtein-Serine-Threonine KinasesSequence AlignmentBody PatterningEndodermGene Expression Regulation, DevelopmentalDuring early vertebrate development, the correct establishment of the body axes is critical. The anterior pole of the mouse embryo is established when Distal Visceral Endoderm (DVE) cells migrate to form the Anterior Visceral Endoderm (AVE). Symmetrical expression of Lefty1, Cer1 and Dkk1 determines the direction of DVE migration and the future anterior side. In addition to the establishment of the Anterior-Posterior axis, the AVE has also been implicated in anterior neural specification. To better understand the role of the AVE in these processes, we have performed a differential screening using Affymetrix GeneChip technology with AVE cells isolated from cer1P-EGFP transgenic mouse embryos. We found 175 genes which were upregulated in the AVE and 36 genes in the Proximal-posterior sample. Using DAVID software, we characterized the AVE cell population regarding cellular component, molecular function and biological processes. Among the genes that were found to be upregulated in the AVE, several novel genes were identified. Four of these transcripts displaying high-fold change in the AVE were further characterized by in situ hybridization in early stages of development in order to validate the screening. From those four selected genes, one, denominated Adtk1, was chosen to be functionally characterized by targeted inactivation in ES cells. Adtk1 encodes for a serine/threonine kinase. Adtk1 null mutants are smaller and present short limbs due to decreased mineralization, suggesting a potential role in chondrogenesis during limb development. Taken together, these data point to the importance of reporting novel genes present in the AVE.Fundação para a Ciência e a Tecnologia PhD fellowships and research grants; Instituto Gulbenkian de Ciência; Fundação Calouste Gulbenkian; IBB/CBME.University of the Basque Country PressARCAGoncalves, LisaFilipe, MarioMarques, SaraSalgueiro, Ana-MarisaBecker, Jorg D.Belo, Jose Antonio2016-08-18T14:40:35Z2011-05-062011-05-06T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfapplication/pdfhttp://hdl.handle.net/10400.7/692engGonçalves, L., Filipe, M., Marques, S., Salgueiro, A.-M., Becker, J. D., Belo, J. A. (2011). Identification and functional analysis of novel genes expressed in the Anterior Visceral Endoderm. Int. J. Dev. Biol., 55(3), 281–295.10.1387/ijdb.103273lginfo:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2022-11-29T14:35:04Zoai:arca.igc.gulbenkian.pt:10400.7/692Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-19T16:11:55.500369Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse |
dc.title.none.fl_str_mv |
Identification and functional analysis of novel genes expressed in the Anterior Visceral Endoderm |
title |
Identification and functional analysis of novel genes expressed in the Anterior Visceral Endoderm |
spellingShingle |
Identification and functional analysis of novel genes expressed in the Anterior Visceral Endoderm Goncalves, Lisa Amino Acid Sequence Animals Cell Movement Chondrogenesis Cytokines Embryo, Mammalian Intercellular Signaling Peptides and Proteins Left-Right Determination Factors Mice Mice, Inbred C57BL Mice, Transgenic Protein-Serine-Threonine Kinases Sequence Alignment Body Patterning Endoderm Gene Expression Regulation, Developmental |
title_short |
Identification and functional analysis of novel genes expressed in the Anterior Visceral Endoderm |
title_full |
Identification and functional analysis of novel genes expressed in the Anterior Visceral Endoderm |
title_fullStr |
Identification and functional analysis of novel genes expressed in the Anterior Visceral Endoderm |
title_full_unstemmed |
Identification and functional analysis of novel genes expressed in the Anterior Visceral Endoderm |
title_sort |
Identification and functional analysis of novel genes expressed in the Anterior Visceral Endoderm |
author |
Goncalves, Lisa |
author_facet |
Goncalves, Lisa Filipe, Mario Marques, Sara Salgueiro, Ana-Marisa Becker, Jorg D. Belo, Jose Antonio |
author_role |
author |
author2 |
Filipe, Mario Marques, Sara Salgueiro, Ana-Marisa Becker, Jorg D. Belo, Jose Antonio |
author2_role |
author author author author author |
dc.contributor.none.fl_str_mv |
ARCA |
dc.contributor.author.fl_str_mv |
Goncalves, Lisa Filipe, Mario Marques, Sara Salgueiro, Ana-Marisa Becker, Jorg D. Belo, Jose Antonio |
dc.subject.por.fl_str_mv |
Amino Acid Sequence Animals Cell Movement Chondrogenesis Cytokines Embryo, Mammalian Intercellular Signaling Peptides and Proteins Left-Right Determination Factors Mice Mice, Inbred C57BL Mice, Transgenic Protein-Serine-Threonine Kinases Sequence Alignment Body Patterning Endoderm Gene Expression Regulation, Developmental |
topic |
Amino Acid Sequence Animals Cell Movement Chondrogenesis Cytokines Embryo, Mammalian Intercellular Signaling Peptides and Proteins Left-Right Determination Factors Mice Mice, Inbred C57BL Mice, Transgenic Protein-Serine-Threonine Kinases Sequence Alignment Body Patterning Endoderm Gene Expression Regulation, Developmental |
description |
During early vertebrate development, the correct establishment of the body axes is critical. The anterior pole of the mouse embryo is established when Distal Visceral Endoderm (DVE) cells migrate to form the Anterior Visceral Endoderm (AVE). Symmetrical expression of Lefty1, Cer1 and Dkk1 determines the direction of DVE migration and the future anterior side. In addition to the establishment of the Anterior-Posterior axis, the AVE has also been implicated in anterior neural specification. To better understand the role of the AVE in these processes, we have performed a differential screening using Affymetrix GeneChip technology with AVE cells isolated from cer1P-EGFP transgenic mouse embryos. We found 175 genes which were upregulated in the AVE and 36 genes in the Proximal-posterior sample. Using DAVID software, we characterized the AVE cell population regarding cellular component, molecular function and biological processes. Among the genes that were found to be upregulated in the AVE, several novel genes were identified. Four of these transcripts displaying high-fold change in the AVE were further characterized by in situ hybridization in early stages of development in order to validate the screening. From those four selected genes, one, denominated Adtk1, was chosen to be functionally characterized by targeted inactivation in ES cells. Adtk1 encodes for a serine/threonine kinase. Adtk1 null mutants are smaller and present short limbs due to decreased mineralization, suggesting a potential role in chondrogenesis during limb development. Taken together, these data point to the importance of reporting novel genes present in the AVE. |
publishDate |
2011 |
dc.date.none.fl_str_mv |
2011-05-06 2011-05-06T00:00:00Z 2016-08-18T14:40:35Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://hdl.handle.net/10400.7/692 |
url |
http://hdl.handle.net/10400.7/692 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Gonçalves, L., Filipe, M., Marques, S., Salgueiro, A.-M., Becker, J. D., Belo, J. A. (2011). Identification and functional analysis of novel genes expressed in the Anterior Visceral Endoderm. Int. J. Dev. Biol., 55(3), 281–295. 10.1387/ijdb.103273lg |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf application/pdf |
dc.publisher.none.fl_str_mv |
University of the Basque Country Press |
publisher.none.fl_str_mv |
University of the Basque Country Press |
dc.source.none.fl_str_mv |
reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação instacron:RCAAP |
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Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
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RCAAP |
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RCAAP |
reponame_str |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
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Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
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Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
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1799130574688878592 |