Concerted involvement of Cdx/Hox genes and Wnt signaling in morphogenesis of the caudal neural tube and cloacal derivatives from the posterior growth zone
Autor(a) principal: | |
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Data de Publicação: | 2011 |
Outros Autores: | , , , , , , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
Texto Completo: | http://hdl.handle.net/10400.7/644 |
Resumo: | Decrease in Cdx dosage in an allelic series of mouse Cdx mutants leads to progressively more severe posterior vertebral defects. These defects are corrected by posterior gain of function of the Wnt effector Lef1. Precocious expression of Hox paralogous 13 genes also induces vertebral axis truncation by antagonizing Cdx function. We report here that the phenotypic similarity also applies to patterning of the caudal neural tube and uro-rectal tracts in Cdx and Wnt3a mutants, and in embryos precociously expressing Hox13 genes. Cdx2 inactivation after placentation leads to posterior defects, including incomplete uro-rectal septation. Compound mutants carrying one active Cdx2 allele in the Cdx4-null background (Cdx2/4), transgenic embryos precociously expressing Hox13 genes and a novel Wnt3a hypomorph mutant all manifest a comparable phenotype with similar uro-rectal defects. Phenotype and transcriptome analysis in early Cdx mutants, genetic rescue experiments and gene expression studies lead us to propose that Cdx transcription factors act via Wnt signaling during the laying down of uro-rectal mesoderm, and that they are operative in an early phase of these events, at the site of tissue progenitors in the posterior growth zone of the embryo. Cdx and Wnt mutations and premature Hox13 expression also cause similar neural dysmorphology, including ectopic neural structures that sometimes lead to neural tube splitting at caudal axial levels. These findings involve the Cdx genes, canonical Wnt signaling and the temporal control of posterior Hox gene expression in posterior morphogenesis in the different embryonic germ layers. They shed a new light on the etiology of the caudal dysplasia or caudal regression range of human congenital defects. |
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Concerted involvement of Cdx/Hox genes and Wnt signaling in morphogenesis of the caudal neural tube and cloacal derivatives from the posterior growth zoneAnimalsCell ShapeEmbryo, MammalianFemaleHedgehog ProteinsHomeodomain ProteinsMaleMiceMice, Inbred C57BLMice, TransgenicNeural TubeTranscription FactorsTretinoinWnt ProteinsWnt3 ProteinWnt3A ProteinGene Expression Regulation, DevelopmentalSignal TransductionDecrease in Cdx dosage in an allelic series of mouse Cdx mutants leads to progressively more severe posterior vertebral defects. These defects are corrected by posterior gain of function of the Wnt effector Lef1. Precocious expression of Hox paralogous 13 genes also induces vertebral axis truncation by antagonizing Cdx function. We report here that the phenotypic similarity also applies to patterning of the caudal neural tube and uro-rectal tracts in Cdx and Wnt3a mutants, and in embryos precociously expressing Hox13 genes. Cdx2 inactivation after placentation leads to posterior defects, including incomplete uro-rectal septation. Compound mutants carrying one active Cdx2 allele in the Cdx4-null background (Cdx2/4), transgenic embryos precociously expressing Hox13 genes and a novel Wnt3a hypomorph mutant all manifest a comparable phenotype with similar uro-rectal defects. Phenotype and transcriptome analysis in early Cdx mutants, genetic rescue experiments and gene expression studies lead us to propose that Cdx transcription factors act via Wnt signaling during the laying down of uro-rectal mesoderm, and that they are operative in an early phase of these events, at the site of tissue progenitors in the posterior growth zone of the embryo. Cdx and Wnt mutations and premature Hox13 expression also cause similar neural dysmorphology, including ectopic neural structures that sometimes lead to neural tube splitting at caudal axial levels. These findings involve the Cdx genes, canonical Wnt signaling and the temporal control of posterior Hox gene expression in posterior morphogenesis in the different embryonic germ layers. They shed a new light on the etiology of the caudal dysplasia or caudal regression range of human congenital defects.AICR project grant: (08-0199); Dutch Earth and Life Sciences grant: (820.02.005); 6th Framework Programme Network of Excellence `Cells into Organs'; Dutch government grant: (Bsik Program 03038); Fundação para a Ciência e Tecnologia grant: (PTDC/BIA-BCM/110638/2009); Centro de Biologia do Desenvolvimento grant: (POCTI-ISFL-4-664).Company of BiologistsARCAvan de Ven, C.Bialecka, M.Neijts, R.Young, T.Rowland, J. E.Stringer, E. J.Van Rooijen, C.Meijlink, F.Novoa, A.Freund, J.-N.Mallo, M.Beck, F.Deschamps, J.2016-06-14T14:45:28Z2011-07-312011-07-31T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfapplication/pdfapplication/pdfapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/10400.7/644engConcerted involvement of Cdx/Hox genes and Wnt signaling in morphogenesis of the caudal neural tube and cloacal derivatives from the posterior growth zone Cesca van de Ven, Monika Bialecka, Roel Neijts, Teddy Young, Jennifer E. Rowland, Emma J. Stringer, Carina Van Rooijen, Frits Meijlink, Ana Nóvoa, Jean-Noel Freund, Moises Mallo, Felix Beck, Jacqueline Deschamps Development 2011 138: 3451-3462; doi: 10.1242/dev.06611810.1242/dev.066118info:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2022-11-29T14:35:02Zoai:arca.igc.gulbenkian.pt:10400.7/644Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-19T16:11:53.297242Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse |
dc.title.none.fl_str_mv |
Concerted involvement of Cdx/Hox genes and Wnt signaling in morphogenesis of the caudal neural tube and cloacal derivatives from the posterior growth zone |
title |
Concerted involvement of Cdx/Hox genes and Wnt signaling in morphogenesis of the caudal neural tube and cloacal derivatives from the posterior growth zone |
spellingShingle |
Concerted involvement of Cdx/Hox genes and Wnt signaling in morphogenesis of the caudal neural tube and cloacal derivatives from the posterior growth zone van de Ven, C. Animals Cell Shape Embryo, Mammalian Female Hedgehog Proteins Homeodomain Proteins Male Mice Mice, Inbred C57BL Mice, Transgenic Neural Tube Transcription Factors Tretinoin Wnt Proteins Wnt3 Protein Wnt3A Protein Gene Expression Regulation, Developmental Signal Transduction |
title_short |
Concerted involvement of Cdx/Hox genes and Wnt signaling in morphogenesis of the caudal neural tube and cloacal derivatives from the posterior growth zone |
title_full |
Concerted involvement of Cdx/Hox genes and Wnt signaling in morphogenesis of the caudal neural tube and cloacal derivatives from the posterior growth zone |
title_fullStr |
Concerted involvement of Cdx/Hox genes and Wnt signaling in morphogenesis of the caudal neural tube and cloacal derivatives from the posterior growth zone |
title_full_unstemmed |
Concerted involvement of Cdx/Hox genes and Wnt signaling in morphogenesis of the caudal neural tube and cloacal derivatives from the posterior growth zone |
title_sort |
Concerted involvement of Cdx/Hox genes and Wnt signaling in morphogenesis of the caudal neural tube and cloacal derivatives from the posterior growth zone |
author |
van de Ven, C. |
author_facet |
van de Ven, C. Bialecka, M. Neijts, R. Young, T. Rowland, J. E. Stringer, E. J. Van Rooijen, C. Meijlink, F. Novoa, A. Freund, J.-N. Mallo, M. Beck, F. Deschamps, J. |
author_role |
author |
author2 |
Bialecka, M. Neijts, R. Young, T. Rowland, J. E. Stringer, E. J. Van Rooijen, C. Meijlink, F. Novoa, A. Freund, J.-N. Mallo, M. Beck, F. Deschamps, J. |
author2_role |
author author author author author author author author author author author author |
dc.contributor.none.fl_str_mv |
ARCA |
dc.contributor.author.fl_str_mv |
van de Ven, C. Bialecka, M. Neijts, R. Young, T. Rowland, J. E. Stringer, E. J. Van Rooijen, C. Meijlink, F. Novoa, A. Freund, J.-N. Mallo, M. Beck, F. Deschamps, J. |
dc.subject.por.fl_str_mv |
Animals Cell Shape Embryo, Mammalian Female Hedgehog Proteins Homeodomain Proteins Male Mice Mice, Inbred C57BL Mice, Transgenic Neural Tube Transcription Factors Tretinoin Wnt Proteins Wnt3 Protein Wnt3A Protein Gene Expression Regulation, Developmental Signal Transduction |
topic |
Animals Cell Shape Embryo, Mammalian Female Hedgehog Proteins Homeodomain Proteins Male Mice Mice, Inbred C57BL Mice, Transgenic Neural Tube Transcription Factors Tretinoin Wnt Proteins Wnt3 Protein Wnt3A Protein Gene Expression Regulation, Developmental Signal Transduction |
description |
Decrease in Cdx dosage in an allelic series of mouse Cdx mutants leads to progressively more severe posterior vertebral defects. These defects are corrected by posterior gain of function of the Wnt effector Lef1. Precocious expression of Hox paralogous 13 genes also induces vertebral axis truncation by antagonizing Cdx function. We report here that the phenotypic similarity also applies to patterning of the caudal neural tube and uro-rectal tracts in Cdx and Wnt3a mutants, and in embryos precociously expressing Hox13 genes. Cdx2 inactivation after placentation leads to posterior defects, including incomplete uro-rectal septation. Compound mutants carrying one active Cdx2 allele in the Cdx4-null background (Cdx2/4), transgenic embryos precociously expressing Hox13 genes and a novel Wnt3a hypomorph mutant all manifest a comparable phenotype with similar uro-rectal defects. Phenotype and transcriptome analysis in early Cdx mutants, genetic rescue experiments and gene expression studies lead us to propose that Cdx transcription factors act via Wnt signaling during the laying down of uro-rectal mesoderm, and that they are operative in an early phase of these events, at the site of tissue progenitors in the posterior growth zone of the embryo. Cdx and Wnt mutations and premature Hox13 expression also cause similar neural dysmorphology, including ectopic neural structures that sometimes lead to neural tube splitting at caudal axial levels. These findings involve the Cdx genes, canonical Wnt signaling and the temporal control of posterior Hox gene expression in posterior morphogenesis in the different embryonic germ layers. They shed a new light on the etiology of the caudal dysplasia or caudal regression range of human congenital defects. |
publishDate |
2011 |
dc.date.none.fl_str_mv |
2011-07-31 2011-07-31T00:00:00Z 2016-06-14T14:45:28Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://hdl.handle.net/10400.7/644 |
url |
http://hdl.handle.net/10400.7/644 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Concerted involvement of Cdx/Hox genes and Wnt signaling in morphogenesis of the caudal neural tube and cloacal derivatives from the posterior growth zone Cesca van de Ven, Monika Bialecka, Roel Neijts, Teddy Young, Jennifer E. Rowland, Emma J. Stringer, Carina Van Rooijen, Frits Meijlink, Ana Nóvoa, Jean-Noel Freund, Moises Mallo, Felix Beck, Jacqueline Deschamps Development 2011 138: 3451-3462; doi: 10.1242/dev.066118 10.1242/dev.066118 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
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openAccess |
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application/pdf application/pdf application/pdf application/pdf application/pdf application/pdf |
dc.publisher.none.fl_str_mv |
Company of Biologists |
publisher.none.fl_str_mv |
Company of Biologists |
dc.source.none.fl_str_mv |
reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação instacron:RCAAP |
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Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
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RCAAP |
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Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
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Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
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Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
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1799130573829046272 |