Injectable gellan-gum/hydroxyapatite-based bilayered hydrogel composites for osteochondral tissue regeneration

Detalhes bibliográficos
Autor(a) principal: Pereira, D. R.
Data de Publicação: 2018
Outros Autores: Canadas, R. F., Silva-Correia, J., da Silva Morais, A., Oliveira, M. B., Dias, I. R., Mano, J. F., Marques, A. P., Reis, R. L., Oliveira, J. M.
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
Texto Completo: http://hdl.handle.net/1822/56299
Resumo: Multilayer systems capable of simultaneous dual tissue formation are crucial for regeneration of the osteochondral (OC) unit. Despite the tremendous effort in the field there is still no widely accepted system that stands out in terms of superior OC regeneration. Herein, we developed bilayered hydrogel composites (BHC) combining two structurally stratified layers fabricated from naturally derived and synthetic polymers, gellan-gum (GG) and hydroxyapatite (HAp), respectively. Two formulations were made from either low acyl GG (LAGG) alone or in combination with high acyl GG (HAGG) for the cartilage-like layer. Four bone-like layers were made of LAGG incorporating different ratios of hydroxyapatite (HAp). BHC were assembled in one single construct resulting in eight distinct bilayered constructs. Architectural observations by stereomicroscope and micro-CT (μ-CT) demonstrated a connected stratified structure with good ceramic dispersion within the bone-like layer. Swelling and degradation tests as well mechanical analyse showed a stable viscoelastic construct under dynamic forces. In-vitro studies by encapsulating rabbit's chondrocytes and osteoblasts in the respective layers showed the cytocompatibility of the BHC. Further studies comprising subcutaneous implantation in mice displayed a weak immune response after four weeks. OC orthotopic defects in the rabbit's knee were created and injected with the acellular BHC. OC tissue was regenerated four weeks after implantation as confirmed by cartilaginous and bony tissue formation assessed by histologic staining and μ-CT analysis. The successful fabrication of injectable BHC and their in-vitro and in-vivo performance may be seen as advanced engineered platforms to treat the challenging OC defects.
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spelling Injectable gellan-gum/hydroxyapatite-based bilayered hydrogel composites for osteochondral tissue regenerationbilayered hydrogel compositesGellan-gumhydroxyapatiteInjectable biomaterialsorthotopic knee modelOsteochondral regenerationScience & TechnologyMultilayer systems capable of simultaneous dual tissue formation are crucial for regeneration of the osteochondral (OC) unit. Despite the tremendous effort in the field there is still no widely accepted system that stands out in terms of superior OC regeneration. Herein, we developed bilayered hydrogel composites (BHC) combining two structurally stratified layers fabricated from naturally derived and synthetic polymers, gellan-gum (GG) and hydroxyapatite (HAp), respectively. Two formulations were made from either low acyl GG (LAGG) alone or in combination with high acyl GG (HAGG) for the cartilage-like layer. Four bone-like layers were made of LAGG incorporating different ratios of hydroxyapatite (HAp). BHC were assembled in one single construct resulting in eight distinct bilayered constructs. Architectural observations by stereomicroscope and micro-CT (μ-CT) demonstrated a connected stratified structure with good ceramic dispersion within the bone-like layer. Swelling and degradation tests as well mechanical analyse showed a stable viscoelastic construct under dynamic forces. In-vitro studies by encapsulating rabbit's chondrocytes and osteoblasts in the respective layers showed the cytocompatibility of the BHC. Further studies comprising subcutaneous implantation in mice displayed a weak immune response after four weeks. OC orthotopic defects in the rabbit's knee were created and injected with the acellular BHC. OC tissue was regenerated four weeks after implantation as confirmed by cartilaginous and bony tissue formation assessed by histologic staining and μ-CT analysis. The successful fabrication of injectable BHC and their in-vitro and in-vivo performance may be seen as advanced engineered platforms to treat the challenging OC defects.This material is based on works supported by the Portuguese Foundation for Science and Technology (FCT) under the OsteoCart project (PTDC/CTM-BPC/115977/2009) and for the M-ERA-NET/0001/2014 project. The authors are grateful to Teresa Oliveira for the assistance with histological studies. DR Pereira acknowledges the FCT for the individual grant (SFRH/BD/81356/2011) and JM Oliveira also thanks the FCT for the funds provided under the program Investigator FTC 2012 and 2015 (IF/00423/2012 and IF/01285/2015).info:eu-repo/semantics/publishedVersionElsevierUniversidade do MinhoPereira, D. R.Canadas, R. F.Silva-Correia, J.da Silva Morais, A.Oliveira, M. B.Dias, I. R.Mano, J. F.Marques, A. P.Reis, R. L.Oliveira, J. M.2018-072018-07-01T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/1822/56299engPereira D. R., Canadas R. F., Silva-Correia J., da Silva Morais A., Oliveira M. B., Dias I. R., Mano J. F., Marques A. P., Reis R. L., Oliveira J. M. Injectable gellan-gum/hydroxyapatite-based bilayered hydrogel composites for osteochondral tissue regeneration, Applied Materials Today, Vol. 12, pp. 309-321, doi:10.1016/j.apmt.2018.06.005, 20182352-940710.1016/j.apmt.2018.06.005https://www.sciencedirect.com/science/article/pii/S2352940718301616info:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2024-05-11T07:13:04Zoai:repositorium.sdum.uminho.pt:1822/56299Portal AgregadorONGhttps://www.rcaap.pt/oai/openairemluisa.alvim@gmail.comopendoar:71602024-05-11T07:13:04Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse
dc.title.none.fl_str_mv Injectable gellan-gum/hydroxyapatite-based bilayered hydrogel composites for osteochondral tissue regeneration
title Injectable gellan-gum/hydroxyapatite-based bilayered hydrogel composites for osteochondral tissue regeneration
spellingShingle Injectable gellan-gum/hydroxyapatite-based bilayered hydrogel composites for osteochondral tissue regeneration
Pereira, D. R.
bilayered hydrogel composites
Gellan-gum
hydroxyapatite
Injectable biomaterials
orthotopic knee model
Osteochondral regeneration
Science & Technology
title_short Injectable gellan-gum/hydroxyapatite-based bilayered hydrogel composites for osteochondral tissue regeneration
title_full Injectable gellan-gum/hydroxyapatite-based bilayered hydrogel composites for osteochondral tissue regeneration
title_fullStr Injectable gellan-gum/hydroxyapatite-based bilayered hydrogel composites for osteochondral tissue regeneration
title_full_unstemmed Injectable gellan-gum/hydroxyapatite-based bilayered hydrogel composites for osteochondral tissue regeneration
title_sort Injectable gellan-gum/hydroxyapatite-based bilayered hydrogel composites for osteochondral tissue regeneration
author Pereira, D. R.
author_facet Pereira, D. R.
Canadas, R. F.
Silva-Correia, J.
da Silva Morais, A.
Oliveira, M. B.
Dias, I. R.
Mano, J. F.
Marques, A. P.
Reis, R. L.
Oliveira, J. M.
author_role author
author2 Canadas, R. F.
Silva-Correia, J.
da Silva Morais, A.
Oliveira, M. B.
Dias, I. R.
Mano, J. F.
Marques, A. P.
Reis, R. L.
Oliveira, J. M.
author2_role author
author
author
author
author
author
author
author
author
dc.contributor.none.fl_str_mv Universidade do Minho
dc.contributor.author.fl_str_mv Pereira, D. R.
Canadas, R. F.
Silva-Correia, J.
da Silva Morais, A.
Oliveira, M. B.
Dias, I. R.
Mano, J. F.
Marques, A. P.
Reis, R. L.
Oliveira, J. M.
dc.subject.por.fl_str_mv bilayered hydrogel composites
Gellan-gum
hydroxyapatite
Injectable biomaterials
orthotopic knee model
Osteochondral regeneration
Science & Technology
topic bilayered hydrogel composites
Gellan-gum
hydroxyapatite
Injectable biomaterials
orthotopic knee model
Osteochondral regeneration
Science & Technology
description Multilayer systems capable of simultaneous dual tissue formation are crucial for regeneration of the osteochondral (OC) unit. Despite the tremendous effort in the field there is still no widely accepted system that stands out in terms of superior OC regeneration. Herein, we developed bilayered hydrogel composites (BHC) combining two structurally stratified layers fabricated from naturally derived and synthetic polymers, gellan-gum (GG) and hydroxyapatite (HAp), respectively. Two formulations were made from either low acyl GG (LAGG) alone or in combination with high acyl GG (HAGG) for the cartilage-like layer. Four bone-like layers were made of LAGG incorporating different ratios of hydroxyapatite (HAp). BHC were assembled in one single construct resulting in eight distinct bilayered constructs. Architectural observations by stereomicroscope and micro-CT (μ-CT) demonstrated a connected stratified structure with good ceramic dispersion within the bone-like layer. Swelling and degradation tests as well mechanical analyse showed a stable viscoelastic construct under dynamic forces. In-vitro studies by encapsulating rabbit's chondrocytes and osteoblasts in the respective layers showed the cytocompatibility of the BHC. Further studies comprising subcutaneous implantation in mice displayed a weak immune response after four weeks. OC orthotopic defects in the rabbit's knee were created and injected with the acellular BHC. OC tissue was regenerated four weeks after implantation as confirmed by cartilaginous and bony tissue formation assessed by histologic staining and μ-CT analysis. The successful fabrication of injectable BHC and their in-vitro and in-vivo performance may be seen as advanced engineered platforms to treat the challenging OC defects.
publishDate 2018
dc.date.none.fl_str_mv 2018-07
2018-07-01T00:00:00Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://hdl.handle.net/1822/56299
url http://hdl.handle.net/1822/56299
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv Pereira D. R., Canadas R. F., Silva-Correia J., da Silva Morais A., Oliveira M. B., Dias I. R., Mano J. F., Marques A. P., Reis R. L., Oliveira J. M. Injectable gellan-gum/hydroxyapatite-based bilayered hydrogel composites for osteochondral tissue regeneration, Applied Materials Today, Vol. 12, pp. 309-321, doi:10.1016/j.apmt.2018.06.005, 2018
2352-9407
10.1016/j.apmt.2018.06.005
https://www.sciencedirect.com/science/article/pii/S2352940718301616
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Elsevier
publisher.none.fl_str_mv Elsevier
dc.source.none.fl_str_mv reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron:RCAAP
instname_str Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron_str RCAAP
institution RCAAP
reponame_str Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
collection Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
repository.name.fl_str_mv Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
repository.mail.fl_str_mv mluisa.alvim@gmail.com
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