Gellan gum-based hydrogel bilayered scaffolds for osteochondral tissue engineering
Autor(a) principal: | |
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Data de Publicação: | 2014 |
Outros Autores: | , , , , |
Idioma: | eng |
Título da fonte: | Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
Texto Completo: | http://hdl.handle.net/1822/28184 |
Resumo: | It has been shown that hydrogel bilayered scaffolds combining cartilage- and bone-like layers are most advantageous for treating osteochondral defects. In this study, it is proposed the use of low acyl gellan gum (LAGG) for developing bilayered hydrogel scaffolds for osteochondral tissue engineering. The cartilage-like layer of the GG-based bilayered hydrogel scaffolds is composed of LAGG (2 wt%). By adding a 2 wt% LAGG aqueous solution to different amounts of HAp (5-20 wt%) it was possible to produce the bone-like layer. In vitro bioactivity tests were performed by means of soaking the LAGG/LAGG-HAp hydrogel scaffolds in a simulated body fluid solution up to 14 days. Scanning electron microscopy, Fourier transform infra-red spectroscopy and X-ray diffraction analyses demonstrated that apatite formation is limited to the bone-like layer of the LAGG/LAGG-HAp bilayered hydrogel scaffolds. |
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Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
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7160 |
spelling |
Gellan gum-based hydrogel bilayered scaffolds for osteochondral tissue engineeringBilayered scaffoldsGellan gumHydrogelsOsteochondralTissue engineeringScience & TechnologyIt has been shown that hydrogel bilayered scaffolds combining cartilage- and bone-like layers are most advantageous for treating osteochondral defects. In this study, it is proposed the use of low acyl gellan gum (LAGG) for developing bilayered hydrogel scaffolds for osteochondral tissue engineering. The cartilage-like layer of the GG-based bilayered hydrogel scaffolds is composed of LAGG (2 wt%). By adding a 2 wt% LAGG aqueous solution to different amounts of HAp (5-20 wt%) it was possible to produce the bone-like layer. In vitro bioactivity tests were performed by means of soaking the LAGG/LAGG-HAp hydrogel scaffolds in a simulated body fluid solution up to 14 days. Scanning electron microscopy, Fourier transform infra-red spectroscopy and X-ray diffraction analyses demonstrated that apatite formation is limited to the bone-like layer of the LAGG/LAGG-HAp bilayered hydrogel scaffolds.Trans Tech PublicationsUniversidade do MinhoPereira, D. R.Canadas, Raphael FaustinoSilva-Correia, JoanaMarques, A. P.Reis, R. L.Oliveira, Joaquim M.2014-012014-01-01T00:00:00Zconference paperinfo:eu-repo/semantics/publishedVersionapplication/pdfhttp://hdl.handle.net/1822/28184eng1662-979510.4028/www.scientific.net/KEM.587.255http://www.scientific.net/KEM.587.255info:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2024-05-11T06:40:59Zoai:repositorium.sdum.uminho.pt:1822/28184Portal AgregadorONGhttps://www.rcaap.pt/oai/openairemluisa.alvim@gmail.comopendoar:71602024-05-11T06:40:59Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse |
dc.title.none.fl_str_mv |
Gellan gum-based hydrogel bilayered scaffolds for osteochondral tissue engineering |
title |
Gellan gum-based hydrogel bilayered scaffolds for osteochondral tissue engineering |
spellingShingle |
Gellan gum-based hydrogel bilayered scaffolds for osteochondral tissue engineering Pereira, D. R. Bilayered scaffolds Gellan gum Hydrogels Osteochondral Tissue engineering Science & Technology |
title_short |
Gellan gum-based hydrogel bilayered scaffolds for osteochondral tissue engineering |
title_full |
Gellan gum-based hydrogel bilayered scaffolds for osteochondral tissue engineering |
title_fullStr |
Gellan gum-based hydrogel bilayered scaffolds for osteochondral tissue engineering |
title_full_unstemmed |
Gellan gum-based hydrogel bilayered scaffolds for osteochondral tissue engineering |
title_sort |
Gellan gum-based hydrogel bilayered scaffolds for osteochondral tissue engineering |
author |
Pereira, D. R. |
author_facet |
Pereira, D. R. Canadas, Raphael Faustino Silva-Correia, Joana Marques, A. P. Reis, R. L. Oliveira, Joaquim M. |
author_role |
author |
author2 |
Canadas, Raphael Faustino Silva-Correia, Joana Marques, A. P. Reis, R. L. Oliveira, Joaquim M. |
author2_role |
author author author author author |
dc.contributor.none.fl_str_mv |
Universidade do Minho |
dc.contributor.author.fl_str_mv |
Pereira, D. R. Canadas, Raphael Faustino Silva-Correia, Joana Marques, A. P. Reis, R. L. Oliveira, Joaquim M. |
dc.subject.por.fl_str_mv |
Bilayered scaffolds Gellan gum Hydrogels Osteochondral Tissue engineering Science & Technology |
topic |
Bilayered scaffolds Gellan gum Hydrogels Osteochondral Tissue engineering Science & Technology |
description |
It has been shown that hydrogel bilayered scaffolds combining cartilage- and bone-like layers are most advantageous for treating osteochondral defects. In this study, it is proposed the use of low acyl gellan gum (LAGG) for developing bilayered hydrogel scaffolds for osteochondral tissue engineering. The cartilage-like layer of the GG-based bilayered hydrogel scaffolds is composed of LAGG (2 wt%). By adding a 2 wt% LAGG aqueous solution to different amounts of HAp (5-20 wt%) it was possible to produce the bone-like layer. In vitro bioactivity tests were performed by means of soaking the LAGG/LAGG-HAp hydrogel scaffolds in a simulated body fluid solution up to 14 days. Scanning electron microscopy, Fourier transform infra-red spectroscopy and X-ray diffraction analyses demonstrated that apatite formation is limited to the bone-like layer of the LAGG/LAGG-HAp bilayered hydrogel scaffolds. |
publishDate |
2014 |
dc.date.none.fl_str_mv |
2014-01 2014-01-01T00:00:00Z |
dc.type.driver.fl_str_mv |
conference paper |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://hdl.handle.net/1822/28184 |
url |
http://hdl.handle.net/1822/28184 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
1662-9795 10.4028/www.scientific.net/KEM.587.255 http://www.scientific.net/KEM.587.255 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.publisher.none.fl_str_mv |
Trans Tech Publications |
publisher.none.fl_str_mv |
Trans Tech Publications |
dc.source.none.fl_str_mv |
reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação instacron:RCAAP |
instname_str |
Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
instacron_str |
RCAAP |
institution |
RCAAP |
reponame_str |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
collection |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
repository.name.fl_str_mv |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
repository.mail.fl_str_mv |
mluisa.alvim@gmail.com |
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1817545056458899456 |