Gellan gum-based hydrogel bilayered scaffolds for osteochondral tissue engineering

Detalhes bibliográficos
Autor(a) principal: Pereira, D. R.
Data de Publicação: 2014
Outros Autores: Canadas, Raphael Faustino, Silva-Correia, Joana, Marques, A. P., Reis, R. L., Oliveira, Joaquim M.
Idioma: eng
Título da fonte: Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
Texto Completo: http://hdl.handle.net/1822/28184
Resumo: It has been shown that hydrogel bilayered scaffolds combining cartilage- and bone-like layers are most advantageous for treating osteochondral defects. In this study, it is proposed the use of low acyl gellan gum (LAGG) for developing bilayered hydrogel scaffolds for osteochondral tissue engineering. The cartilage-like layer of the GG-based bilayered hydrogel scaffolds is composed of LAGG (2 wt%). By adding a 2 wt% LAGG aqueous solution to different amounts of HAp (5-20 wt%) it was possible to produce the bone-like layer. In vitro bioactivity tests were performed by means of soaking the LAGG/LAGG-HAp hydrogel scaffolds in a simulated body fluid solution up to 14 days. Scanning electron microscopy, Fourier transform infra-red spectroscopy and X-ray diffraction analyses demonstrated that apatite formation is limited to the bone-like layer of the LAGG/LAGG-HAp bilayered hydrogel scaffolds.
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spelling Gellan gum-based hydrogel bilayered scaffolds for osteochondral tissue engineeringBilayered scaffoldsGellan gumHydrogelsOsteochondralTissue engineeringScience & TechnologyIt has been shown that hydrogel bilayered scaffolds combining cartilage- and bone-like layers are most advantageous for treating osteochondral defects. In this study, it is proposed the use of low acyl gellan gum (LAGG) for developing bilayered hydrogel scaffolds for osteochondral tissue engineering. The cartilage-like layer of the GG-based bilayered hydrogel scaffolds is composed of LAGG (2 wt%). By adding a 2 wt% LAGG aqueous solution to different amounts of HAp (5-20 wt%) it was possible to produce the bone-like layer. In vitro bioactivity tests were performed by means of soaking the LAGG/LAGG-HAp hydrogel scaffolds in a simulated body fluid solution up to 14 days. Scanning electron microscopy, Fourier transform infra-red spectroscopy and X-ray diffraction analyses demonstrated that apatite formation is limited to the bone-like layer of the LAGG/LAGG-HAp bilayered hydrogel scaffolds.Trans Tech PublicationsUniversidade do MinhoPereira, D. R.Canadas, Raphael FaustinoSilva-Correia, JoanaMarques, A. P.Reis, R. L.Oliveira, Joaquim M.2014-012014-01-01T00:00:00Zconference paperinfo:eu-repo/semantics/publishedVersionapplication/pdfhttp://hdl.handle.net/1822/28184eng1662-979510.4028/www.scientific.net/KEM.587.255http://www.scientific.net/KEM.587.255info:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2024-05-11T06:40:59Zoai:repositorium.sdum.uminho.pt:1822/28184Portal AgregadorONGhttps://www.rcaap.pt/oai/openairemluisa.alvim@gmail.comopendoar:71602024-05-11T06:40:59Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse
dc.title.none.fl_str_mv Gellan gum-based hydrogel bilayered scaffolds for osteochondral tissue engineering
title Gellan gum-based hydrogel bilayered scaffolds for osteochondral tissue engineering
spellingShingle Gellan gum-based hydrogel bilayered scaffolds for osteochondral tissue engineering
Pereira, D. R.
Bilayered scaffolds
Gellan gum
Hydrogels
Osteochondral
Tissue engineering
Science & Technology
title_short Gellan gum-based hydrogel bilayered scaffolds for osteochondral tissue engineering
title_full Gellan gum-based hydrogel bilayered scaffolds for osteochondral tissue engineering
title_fullStr Gellan gum-based hydrogel bilayered scaffolds for osteochondral tissue engineering
title_full_unstemmed Gellan gum-based hydrogel bilayered scaffolds for osteochondral tissue engineering
title_sort Gellan gum-based hydrogel bilayered scaffolds for osteochondral tissue engineering
author Pereira, D. R.
author_facet Pereira, D. R.
Canadas, Raphael Faustino
Silva-Correia, Joana
Marques, A. P.
Reis, R. L.
Oliveira, Joaquim M.
author_role author
author2 Canadas, Raphael Faustino
Silva-Correia, Joana
Marques, A. P.
Reis, R. L.
Oliveira, Joaquim M.
author2_role author
author
author
author
author
dc.contributor.none.fl_str_mv Universidade do Minho
dc.contributor.author.fl_str_mv Pereira, D. R.
Canadas, Raphael Faustino
Silva-Correia, Joana
Marques, A. P.
Reis, R. L.
Oliveira, Joaquim M.
dc.subject.por.fl_str_mv Bilayered scaffolds
Gellan gum
Hydrogels
Osteochondral
Tissue engineering
Science & Technology
topic Bilayered scaffolds
Gellan gum
Hydrogels
Osteochondral
Tissue engineering
Science & Technology
description It has been shown that hydrogel bilayered scaffolds combining cartilage- and bone-like layers are most advantageous for treating osteochondral defects. In this study, it is proposed the use of low acyl gellan gum (LAGG) for developing bilayered hydrogel scaffolds for osteochondral tissue engineering. The cartilage-like layer of the GG-based bilayered hydrogel scaffolds is composed of LAGG (2 wt%). By adding a 2 wt% LAGG aqueous solution to different amounts of HAp (5-20 wt%) it was possible to produce the bone-like layer. In vitro bioactivity tests were performed by means of soaking the LAGG/LAGG-HAp hydrogel scaffolds in a simulated body fluid solution up to 14 days. Scanning electron microscopy, Fourier transform infra-red spectroscopy and X-ray diffraction analyses demonstrated that apatite formation is limited to the bone-like layer of the LAGG/LAGG-HAp bilayered hydrogel scaffolds.
publishDate 2014
dc.date.none.fl_str_mv 2014-01
2014-01-01T00:00:00Z
dc.type.driver.fl_str_mv conference paper
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://hdl.handle.net/1822/28184
url http://hdl.handle.net/1822/28184
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv 1662-9795
10.4028/www.scientific.net/KEM.587.255
http://www.scientific.net/KEM.587.255
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Trans Tech Publications
publisher.none.fl_str_mv Trans Tech Publications
dc.source.none.fl_str_mv reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron:RCAAP
instname_str Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron_str RCAAP
institution RCAAP
reponame_str Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
collection Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
repository.name.fl_str_mv Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
repository.mail.fl_str_mv mluisa.alvim@gmail.com
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