Gellan-gum based bilayered scaffolds for application in osteochondral tissue engineering

Detalhes bibliográficos
Autor(a) principal: Pereira, D. R.
Data de Publicação: 2012
Outros Autores: Silva-Correia, Joana, Oliveira, Joaquim M., Pereira, H., Mendes, João Espregueira, Reis, R. L.
Idioma: eng
Título da fonte: Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
Texto Completo: http://hdl.handle.net/1822/27151
Resumo: Publicado em "Journal ofTissue Engineering and Regenerative Medicine", vol. 7, supp. 1 (2013)
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spelling Gellan-gum based bilayered scaffolds for application in osteochondral tissue engineeringGellan GumOsteochondralPublicado em "Journal ofTissue Engineering and Regenerative Medicine", vol. 7, supp. 1 (2013)Bilayered scaffold and cellular strategies are currently applied to solve the challenging problem of osteochondral defects. In this study, several formulations of Gellan gum were developed to fabricate different scaf- folds possessing a cartilage-like layer and a bone-like layer. The bone- like layers were obtained by low acyl Gellan gum (LAGG) at 2 wt% and different amounts of hydroxyapatite powders (HAp) (5,10,15 and 20wt%). The cartilage-like layers were obtained by preparing LAGG formulation at 2wt% and formulations of LAGG at 2wt% and high acyl Gellan gum (HAGG) at 0.75wt% at a ratio of 75:25(v%). The visco- elastic measurements were performed using a TRITEC8000B DMA to characterize the mechanical behaviour of the bilayered scaffolds. The effect of the incorporation of different amount of HAp within the bone- like layer on the mechanical properties of the scaffolds was also investi- gated. Degradation and water uptake studies were performed by soak- ing the scaffolds in a phosphate buffered saline solution (pH 7.4) up to 30 days. The bilayered scaffolds were investigated by stereo microscope to evaluate the interface between both layers. The cytotoxicity of the bilayered scaffolds was investigated in vitro using a L929 cell line. In vi- tro studies regarding adhesion, encapsulation and viability of human chondrocytes (cartilage-like layer) and human osteoblasts (bone-like layer) cultured in the bilayered scaffolds were also carried by perform- ing SEM analysis and Live/Dead assays.WileyUniversidade do MinhoPereira, D. R.Silva-Correia, JoanaOliveira, Joaquim M.Pereira, H.Mendes, João EspregueiraReis, R. L.2012-092012-09-01T00:00:00Zconference objectinfo:eu-repo/semantics/publishedVersionapplication/pdfhttp://hdl.handle.net/1822/27151enginfo:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2024-05-11T06:45:15Zoai:repositorium.sdum.uminho.pt:1822/27151Portal AgregadorONGhttps://www.rcaap.pt/oai/openairemluisa.alvim@gmail.comopendoar:71602024-05-11T06:45:15Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse
dc.title.none.fl_str_mv Gellan-gum based bilayered scaffolds for application in osteochondral tissue engineering
title Gellan-gum based bilayered scaffolds for application in osteochondral tissue engineering
spellingShingle Gellan-gum based bilayered scaffolds for application in osteochondral tissue engineering
Pereira, D. R.
Gellan Gum
Osteochondral
title_short Gellan-gum based bilayered scaffolds for application in osteochondral tissue engineering
title_full Gellan-gum based bilayered scaffolds for application in osteochondral tissue engineering
title_fullStr Gellan-gum based bilayered scaffolds for application in osteochondral tissue engineering
title_full_unstemmed Gellan-gum based bilayered scaffolds for application in osteochondral tissue engineering
title_sort Gellan-gum based bilayered scaffolds for application in osteochondral tissue engineering
author Pereira, D. R.
author_facet Pereira, D. R.
Silva-Correia, Joana
Oliveira, Joaquim M.
Pereira, H.
Mendes, João Espregueira
Reis, R. L.
author_role author
author2 Silva-Correia, Joana
Oliveira, Joaquim M.
Pereira, H.
Mendes, João Espregueira
Reis, R. L.
author2_role author
author
author
author
author
dc.contributor.none.fl_str_mv Universidade do Minho
dc.contributor.author.fl_str_mv Pereira, D. R.
Silva-Correia, Joana
Oliveira, Joaquim M.
Pereira, H.
Mendes, João Espregueira
Reis, R. L.
dc.subject.por.fl_str_mv Gellan Gum
Osteochondral
topic Gellan Gum
Osteochondral
description Publicado em "Journal ofTissue Engineering and Regenerative Medicine", vol. 7, supp. 1 (2013)
publishDate 2012
dc.date.none.fl_str_mv 2012-09
2012-09-01T00:00:00Z
dc.type.driver.fl_str_mv conference object
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://hdl.handle.net/1822/27151
url http://hdl.handle.net/1822/27151
dc.language.iso.fl_str_mv eng
language eng
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dc.publisher.none.fl_str_mv Wiley
publisher.none.fl_str_mv Wiley
dc.source.none.fl_str_mv reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron:RCAAP
instname_str Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
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reponame_str Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
collection Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
repository.name.fl_str_mv Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
repository.mail.fl_str_mv mluisa.alvim@gmail.com
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