Collagen from Atlantic cod (Gadus morhua) skins extracted using CO2 acidified water with potential application in healthcare
Autor(a) principal: | |
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Data de Publicação: | 2020 |
Outros Autores: | , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
Texto Completo: | http://hdl.handle.net/1822/64114 |
Resumo: | The extraction of collagen from fish skins is being proposed as strategy for valorization of marine origin by-products, being a sustainable alternative to mammal collagen. The method commonly uses solutions of organic acids, but new methodologies are arising, aiming to improve process yields and/or the properties of the resulting products. In this work, skins removed from salt brine Atlantic cod (Gadus morhua) were used to extract collagen, using water acidified with CO2, obtaining an extraction yield of 13.8% (w/w). Acidified water extracted collagen (AWC) presented a total content of proline-like amino acids of 151/1000 residues, with a degree of hydroxylation of 38%, and its SDS-PAGE profile is compatible with type I collagen. Moreover, FTIR, CD and XRD results suggest the presence of preserved triple helix, having a denaturation temperature of 32.3 °C as determined by micro-DSC. AWC exhibited a typical shear thinning behavior, interesting regarding their further processing, namely in jelly-like formulations. Additionally, the presence of AWC in MRC-5 human fibroblasts culture did not affect cell viability, demonstrating the non-cytotoxic behavior. Overall, the results support the efficiency of the proposed approach for collagen extraction and further enable the design of methodologies to address AWC use in biomedical or cosmetic context. |
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Collagen from Atlantic cod (Gadus morhua) skins extracted using CO2 acidified water with potential application in healthcareCO2 acidified waterCod skinsFish collagenGreen extractionMarine biomaterialsupercritical fluidsMarine biomaterialsCO acidified water 2Science & TechnologyThe extraction of collagen from fish skins is being proposed as strategy for valorization of marine origin by-products, being a sustainable alternative to mammal collagen. The method commonly uses solutions of organic acids, but new methodologies are arising, aiming to improve process yields and/or the properties of the resulting products. In this work, skins removed from salt brine Atlantic cod (Gadus morhua) were used to extract collagen, using water acidified with CO2, obtaining an extraction yield of 13.8% (w/w). Acidified water extracted collagen (AWC) presented a total content of proline-like amino acids of 151/1000 residues, with a degree of hydroxylation of 38%, and its SDS-PAGE profile is compatible with type I collagen. Moreover, FTIR, CD and XRD results suggest the presence of preserved triple helix, having a denaturation temperature of 32.3 °C as determined by micro-DSC. AWC exhibited a typical shear thinning behavior, interesting regarding their further processing, namely in jelly-like formulations. Additionally, the presence of AWC in MRC-5 human fibroblasts culture did not affect cell viability, demonstrating the non-cytotoxic behavior. Overall, the results support the efficiency of the proposed approach for collagen extraction and further enable the design of methodologies to address AWC use in biomedical or cosmetic context.This work has received funding from European Union, under the scope of European Regional Development Fund (ERDF) through the Structured Project NORTE-01-0145-FEDER000021 (Norte2020) and under the scope of the European Union Seventh Framework Programme (FP7/2007-2013) through grant agreement ERC-2012-ADG 20120216-321266 (ERC Advanced Grant ComplexiTE). The Portuguese Foundation for Science and Technology is also acknowledged for the grant of A.L.A under Doctoral Programme Do* Mar (PD/BD/127995/2016) and for the PhD grant of C. O. (Norte08-5369-000037) with financial support by Norte 2020. The authors would like to acknowledge Frigoríficos da Ermida, Lda. (Gafanha da Nazaré, Portugal) for the kind offer of Atlantic cod skins and to Dr. Manuela Pintado, Dr. Raquel Madureira and Joana Costa (Universidade Católica Portuguesa, Porto) for the use of microDSC and assistance during measurements. The authors also thank to Dr. Catarina Marques and Dr. Sandra Pina (3B’s Research Group) for assistance on XRD measurements, to Dr. Rita Lopez-Cebral (3B’s Research Group) for assistance on SEC measurements and to Dr. Alexandre Barros for discussions.SpringerUniversidade do MinhoSousa, Rita Alexandra OliveiraMartins, EvaCarvalho, Duarte NunoAlves, Ana Luísa SilvaOliveira, Ana Catarina Freitas SalazarDuarte, Ana Rita C.Silva, Tiago José Quinteiros Lopes HenriquesReis, R. L.2020-022020-02-01T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/1822/64114engSousa R. O., Martins E., Carvalho D. N., Alves A. L., Oliveira C., Duarte A. R. C., Silva T. H., Reis R. L. Collagen from Atlantic cod (Gadus morhua) skins extracted using CO2 acidified water with potential application in healthcare, Journal Of Polymer Research, Vol. 27, Issue 3, doi:10.1007/s10965-020-02048-x, 20201022-976010.1007/s10965-020-02048-xhttps://doi.org/10.1007/s10965-020-02048-xinfo:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2023-07-21T12:46:31Zoai:repositorium.sdum.uminho.pt:1822/64114Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-19T19:44:31.007557Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse |
dc.title.none.fl_str_mv |
Collagen from Atlantic cod (Gadus morhua) skins extracted using CO2 acidified water with potential application in healthcare |
title |
Collagen from Atlantic cod (Gadus morhua) skins extracted using CO2 acidified water with potential application in healthcare |
spellingShingle |
Collagen from Atlantic cod (Gadus morhua) skins extracted using CO2 acidified water with potential application in healthcare Sousa, Rita Alexandra Oliveira CO2 acidified water Cod skins Fish collagen Green extraction Marine biomaterial supercritical fluids Marine biomaterials CO acidified water 2 Science & Technology |
title_short |
Collagen from Atlantic cod (Gadus morhua) skins extracted using CO2 acidified water with potential application in healthcare |
title_full |
Collagen from Atlantic cod (Gadus morhua) skins extracted using CO2 acidified water with potential application in healthcare |
title_fullStr |
Collagen from Atlantic cod (Gadus morhua) skins extracted using CO2 acidified water with potential application in healthcare |
title_full_unstemmed |
Collagen from Atlantic cod (Gadus morhua) skins extracted using CO2 acidified water with potential application in healthcare |
title_sort |
Collagen from Atlantic cod (Gadus morhua) skins extracted using CO2 acidified water with potential application in healthcare |
author |
Sousa, Rita Alexandra Oliveira |
author_facet |
Sousa, Rita Alexandra Oliveira Martins, Eva Carvalho, Duarte Nuno Alves, Ana Luísa Silva Oliveira, Ana Catarina Freitas Salazar Duarte, Ana Rita C. Silva, Tiago José Quinteiros Lopes Henriques Reis, R. L. |
author_role |
author |
author2 |
Martins, Eva Carvalho, Duarte Nuno Alves, Ana Luísa Silva Oliveira, Ana Catarina Freitas Salazar Duarte, Ana Rita C. Silva, Tiago José Quinteiros Lopes Henriques Reis, R. L. |
author2_role |
author author author author author author author |
dc.contributor.none.fl_str_mv |
Universidade do Minho |
dc.contributor.author.fl_str_mv |
Sousa, Rita Alexandra Oliveira Martins, Eva Carvalho, Duarte Nuno Alves, Ana Luísa Silva Oliveira, Ana Catarina Freitas Salazar Duarte, Ana Rita C. Silva, Tiago José Quinteiros Lopes Henriques Reis, R. L. |
dc.subject.por.fl_str_mv |
CO2 acidified water Cod skins Fish collagen Green extraction Marine biomaterial supercritical fluids Marine biomaterials CO acidified water 2 Science & Technology |
topic |
CO2 acidified water Cod skins Fish collagen Green extraction Marine biomaterial supercritical fluids Marine biomaterials CO acidified water 2 Science & Technology |
description |
The extraction of collagen from fish skins is being proposed as strategy for valorization of marine origin by-products, being a sustainable alternative to mammal collagen. The method commonly uses solutions of organic acids, but new methodologies are arising, aiming to improve process yields and/or the properties of the resulting products. In this work, skins removed from salt brine Atlantic cod (Gadus morhua) were used to extract collagen, using water acidified with CO2, obtaining an extraction yield of 13.8% (w/w). Acidified water extracted collagen (AWC) presented a total content of proline-like amino acids of 151/1000 residues, with a degree of hydroxylation of 38%, and its SDS-PAGE profile is compatible with type I collagen. Moreover, FTIR, CD and XRD results suggest the presence of preserved triple helix, having a denaturation temperature of 32.3 °C as determined by micro-DSC. AWC exhibited a typical shear thinning behavior, interesting regarding their further processing, namely in jelly-like formulations. Additionally, the presence of AWC in MRC-5 human fibroblasts culture did not affect cell viability, demonstrating the non-cytotoxic behavior. Overall, the results support the efficiency of the proposed approach for collagen extraction and further enable the design of methodologies to address AWC use in biomedical or cosmetic context. |
publishDate |
2020 |
dc.date.none.fl_str_mv |
2020-02 2020-02-01T00:00:00Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://hdl.handle.net/1822/64114 |
url |
http://hdl.handle.net/1822/64114 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Sousa R. O., Martins E., Carvalho D. N., Alves A. L., Oliveira C., Duarte A. R. C., Silva T. H., Reis R. L. Collagen from Atlantic cod (Gadus morhua) skins extracted using CO2 acidified water with potential application in healthcare, Journal Of Polymer Research, Vol. 27, Issue 3, doi:10.1007/s10965-020-02048-x, 2020 1022-9760 10.1007/s10965-020-02048-x https://doi.org/10.1007/s10965-020-02048-x |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.publisher.none.fl_str_mv |
Springer |
publisher.none.fl_str_mv |
Springer |
dc.source.none.fl_str_mv |
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Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
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Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
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Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
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Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
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