Collagen from Atlantic cod (Gadus morhua) skins extracted using CO2 acidified water with potential application in healthcare

Detalhes bibliográficos
Autor(a) principal: Sousa, Rita O.
Data de Publicação: 2020
Outros Autores: Martins, Eva, Carvalho, Duarte Nuno, Alves, Ana L., Oliveira, Catarina, Duarte, Ana Rita C., Silva, Tiago H., Reis, Rui L.
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
Texto Completo: http://hdl.handle.net/10362/148804
Resumo: This work has received funding from European Union, under the scope of European Regional Development Fund (ERDF) through the Structured Project NORTE-01-0145-FEDER-000021 (Norte2020) and under the scope of the European Union Seventh Framework Programme (FP7/2007-2013) through grant agreement ERC-2012-ADG 20120216-321266 (ERC Advanced Grant ComplexiTE). PhD grant of C. O. (Norte-08-5369-000037) with financial support by Norte 2020. The authors would like to acknowledge Frigoríficos da Ermida, Lda. (Gafanha da Nazaré, Portugal) for the kind offer of Atlantic cod skins and to Dr. Manuela Pintado, Dr. Raquel Madureira and Joana Costa (Universidade Católica Portuguesa, Porto) for the use of microDSC and assistance during measurements. The authors also thank to Dr. Catarina Marques and Dr. Sandra Pina (3B’s Research Group) for assistance on XRD measurements, to Dr. Rita Lopez-Cebral (3B’s Research Group) for assistance on SEC measurements and to Dr. Alexandre Barros for discussions.
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spelling Collagen from Atlantic cod (Gadus morhua) skins extracted using CO2 acidified water with potential application in healthcareCO acidified waterCod skinsFish collagenGreen extractionMarine biomaterialsSupercritical fluidsPolymers and PlasticsOrganic ChemistryMaterials ChemistrySDG 14 - Life Below WaterThis work has received funding from European Union, under the scope of European Regional Development Fund (ERDF) through the Structured Project NORTE-01-0145-FEDER-000021 (Norte2020) and under the scope of the European Union Seventh Framework Programme (FP7/2007-2013) through grant agreement ERC-2012-ADG 20120216-321266 (ERC Advanced Grant ComplexiTE). PhD grant of C. O. (Norte-08-5369-000037) with financial support by Norte 2020. The authors would like to acknowledge Frigoríficos da Ermida, Lda. (Gafanha da Nazaré, Portugal) for the kind offer of Atlantic cod skins and to Dr. Manuela Pintado, Dr. Raquel Madureira and Joana Costa (Universidade Católica Portuguesa, Porto) for the use of microDSC and assistance during measurements. The authors also thank to Dr. Catarina Marques and Dr. Sandra Pina (3B’s Research Group) for assistance on XRD measurements, to Dr. Rita Lopez-Cebral (3B’s Research Group) for assistance on SEC measurements and to Dr. Alexandre Barros for discussions.The extraction of collagen from fish skins is being proposed as strategy for valorization of marine origin by-products, being a sustainable alternative to mammal collagen. The method commonly uses solutions of organic acids, but new methodologies are arising, aiming to improve process yields and/or the properties of the resulting products. In this work, skins removed from salt brine Atlantic cod (Gadus morhua) were used to extract collagen, using water acidified with CO2, obtaining an extraction yield of 13.8% (w/w). Acidified water extracted collagen (AWC) presented a total content of proline-like amino acids of 151/1000 residues, with a degree of hydroxylation of 38%, and its SDS-PAGE profile is compatible with type I collagen. Moreover, FTIR, CD and XRD results suggest the presence of preserved triple helix, having a denaturation temperature of 32.3 °C as determined by micro-DSC. AWC exhibited a typical shear thinning behavior, interesting regarding their further processing, namely in jelly-like formulations. Additionally, the presence of AWC in MRC-5 human fibroblasts culture did not affect cell viability, demonstrating the non-cytotoxic behavior. Overall, the results support the efficiency of the proposed approach for collagen extraction and further enable the design of methodologies to address AWC use in biomedical or cosmetic context.LAQV@REQUIMTEDQ - Departamento de QuímicaRUNSousa, Rita O.Martins, EvaCarvalho, Duarte NunoAlves, Ana L.Oliveira, CatarinaDuarte, Ana Rita C.Silva, Tiago H.Reis, Rui L.2023-02-07T22:21:30Z2020-03-012020-03-01T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/10362/148804eng1022-9760PURE: 31973606https://doi.org/10.1007/s10965-020-02048-xinfo:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2024-03-11T05:30:27Zoai:run.unl.pt:10362/148804Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-20T03:53:29.635562Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse
dc.title.none.fl_str_mv Collagen from Atlantic cod (Gadus morhua) skins extracted using CO2 acidified water with potential application in healthcare
title Collagen from Atlantic cod (Gadus morhua) skins extracted using CO2 acidified water with potential application in healthcare
spellingShingle Collagen from Atlantic cod (Gadus morhua) skins extracted using CO2 acidified water with potential application in healthcare
Sousa, Rita O.
CO acidified water
Cod skins
Fish collagen
Green extraction
Marine biomaterials
Supercritical fluids
Polymers and Plastics
Organic Chemistry
Materials Chemistry
SDG 14 - Life Below Water
title_short Collagen from Atlantic cod (Gadus morhua) skins extracted using CO2 acidified water with potential application in healthcare
title_full Collagen from Atlantic cod (Gadus morhua) skins extracted using CO2 acidified water with potential application in healthcare
title_fullStr Collagen from Atlantic cod (Gadus morhua) skins extracted using CO2 acidified water with potential application in healthcare
title_full_unstemmed Collagen from Atlantic cod (Gadus morhua) skins extracted using CO2 acidified water with potential application in healthcare
title_sort Collagen from Atlantic cod (Gadus morhua) skins extracted using CO2 acidified water with potential application in healthcare
author Sousa, Rita O.
author_facet Sousa, Rita O.
Martins, Eva
Carvalho, Duarte Nuno
Alves, Ana L.
Oliveira, Catarina
Duarte, Ana Rita C.
Silva, Tiago H.
Reis, Rui L.
author_role author
author2 Martins, Eva
Carvalho, Duarte Nuno
Alves, Ana L.
Oliveira, Catarina
Duarte, Ana Rita C.
Silva, Tiago H.
Reis, Rui L.
author2_role author
author
author
author
author
author
author
dc.contributor.none.fl_str_mv LAQV@REQUIMTE
DQ - Departamento de Química
RUN
dc.contributor.author.fl_str_mv Sousa, Rita O.
Martins, Eva
Carvalho, Duarte Nuno
Alves, Ana L.
Oliveira, Catarina
Duarte, Ana Rita C.
Silva, Tiago H.
Reis, Rui L.
dc.subject.por.fl_str_mv CO acidified water
Cod skins
Fish collagen
Green extraction
Marine biomaterials
Supercritical fluids
Polymers and Plastics
Organic Chemistry
Materials Chemistry
SDG 14 - Life Below Water
topic CO acidified water
Cod skins
Fish collagen
Green extraction
Marine biomaterials
Supercritical fluids
Polymers and Plastics
Organic Chemistry
Materials Chemistry
SDG 14 - Life Below Water
description This work has received funding from European Union, under the scope of European Regional Development Fund (ERDF) through the Structured Project NORTE-01-0145-FEDER-000021 (Norte2020) and under the scope of the European Union Seventh Framework Programme (FP7/2007-2013) through grant agreement ERC-2012-ADG 20120216-321266 (ERC Advanced Grant ComplexiTE). PhD grant of C. O. (Norte-08-5369-000037) with financial support by Norte 2020. The authors would like to acknowledge Frigoríficos da Ermida, Lda. (Gafanha da Nazaré, Portugal) for the kind offer of Atlantic cod skins and to Dr. Manuela Pintado, Dr. Raquel Madureira and Joana Costa (Universidade Católica Portuguesa, Porto) for the use of microDSC and assistance during measurements. The authors also thank to Dr. Catarina Marques and Dr. Sandra Pina (3B’s Research Group) for assistance on XRD measurements, to Dr. Rita Lopez-Cebral (3B’s Research Group) for assistance on SEC measurements and to Dr. Alexandre Barros for discussions.
publishDate 2020
dc.date.none.fl_str_mv 2020-03-01
2020-03-01T00:00:00Z
2023-02-07T22:21:30Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://hdl.handle.net/10362/148804
url http://hdl.handle.net/10362/148804
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv 1022-9760
PURE: 31973606
https://doi.org/10.1007/s10965-020-02048-x
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.source.none.fl_str_mv reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron:RCAAP
instname_str Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron_str RCAAP
institution RCAAP
reponame_str Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
collection Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
repository.name.fl_str_mv Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
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