Porous hydrogels from shark skin collagen crosslinked under dense carbon dioxide atmosphere

Detalhes bibliográficos
Autor(a) principal: Silva, S. P.
Data de Publicação: 2013
Outros Autores: Moreira-Silva, J., Silva, Tiago H., Perez-Martin, R. I., Sotelo, C. G., Mano, J. F., Duarte, Ana Rita C., Reis, R. L.
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
Texto Completo: http://hdl.handle.net/1822/25167
Resumo: The possibility to fabricate marine collagen porous structures crosslinked with genipin under high pressure carbon dioxide is investigated. Collagen from shark skin is used to prepare prescaffolds by freeze-drying. The poor stability of the structures and low mechanical properties require crosslinking of the structures. Under dense CO2 atmosphere, crosslinking of collagen pre-scaffolds is allowed for 16 h. Additionally, the hydrogels are foamed and the scaffolds obtained present a highly porous structure. In vitro cell culture tests performed with a chondrocyte-like cell line show good cell adherence and proliferation, which is a strong indication of the potential of these scaffolds to be used in tissue cartilage tissue engineering.
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spelling Porous hydrogels from shark skin collagen crosslinked under dense carbon dioxide atmosphereCollagenHydrogelsMarine biotechnolgoySupercritical fluidsTissue engineeringmarine biotechnologyScience & TechnologyThe possibility to fabricate marine collagen porous structures crosslinked with genipin under high pressure carbon dioxide is investigated. Collagen from shark skin is used to prepare prescaffolds by freeze-drying. The poor stability of the structures and low mechanical properties require crosslinking of the structures. Under dense CO2 atmosphere, crosslinking of collagen pre-scaffolds is allowed for 16 h. Additionally, the hydrogels are foamed and the scaffolds obtained present a highly porous structure. In vitro cell culture tests performed with a chondrocyte-like cell line show good cell adherence and proliferation, which is a strong indication of the potential of these scaffolds to be used in tissue cartilage tissue engineering.The research leading to these results has received funding from the European Union Seventh Framework Programme (FP7/2007-2013) under grant agreement no. KBBE-2010-266033 (project SPECIAL) and from FEDER through POCTEP Project 0330_IBEROMARE_1_P. Portuguese Foundation for Science and Technology is also gratefully acknowledged for post-doc grants of J. Moreira-Silva and T.H. Silva.WileyUniversidade do MinhoSilva, S. P.Moreira-Silva, J.Silva, Tiago H.Perez-Martin, R. I.Sotelo, C. G.Mano, J. F.Duarte, Ana Rita C.Reis, R. L.2013-092013-09-01T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/1822/25167eng1616-519510.1002/mabi.20130022824039034http://onlinelibrary.wiley.com/doi/10.1002/mabi.201300228/abstractinfo:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2023-07-21T12:22:36Zoai:repositorium.sdum.uminho.pt:1822/25167Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-19T19:16:06.345958Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse
dc.title.none.fl_str_mv Porous hydrogels from shark skin collagen crosslinked under dense carbon dioxide atmosphere
title Porous hydrogels from shark skin collagen crosslinked under dense carbon dioxide atmosphere
spellingShingle Porous hydrogels from shark skin collagen crosslinked under dense carbon dioxide atmosphere
Silva, S. P.
Collagen
Hydrogels
Marine biotechnolgoy
Supercritical fluids
Tissue engineering
marine biotechnology
Science & Technology
title_short Porous hydrogels from shark skin collagen crosslinked under dense carbon dioxide atmosphere
title_full Porous hydrogels from shark skin collagen crosslinked under dense carbon dioxide atmosphere
title_fullStr Porous hydrogels from shark skin collagen crosslinked under dense carbon dioxide atmosphere
title_full_unstemmed Porous hydrogels from shark skin collagen crosslinked under dense carbon dioxide atmosphere
title_sort Porous hydrogels from shark skin collagen crosslinked under dense carbon dioxide atmosphere
author Silva, S. P.
author_facet Silva, S. P.
Moreira-Silva, J.
Silva, Tiago H.
Perez-Martin, R. I.
Sotelo, C. G.
Mano, J. F.
Duarte, Ana Rita C.
Reis, R. L.
author_role author
author2 Moreira-Silva, J.
Silva, Tiago H.
Perez-Martin, R. I.
Sotelo, C. G.
Mano, J. F.
Duarte, Ana Rita C.
Reis, R. L.
author2_role author
author
author
author
author
author
author
dc.contributor.none.fl_str_mv Universidade do Minho
dc.contributor.author.fl_str_mv Silva, S. P.
Moreira-Silva, J.
Silva, Tiago H.
Perez-Martin, R. I.
Sotelo, C. G.
Mano, J. F.
Duarte, Ana Rita C.
Reis, R. L.
dc.subject.por.fl_str_mv Collagen
Hydrogels
Marine biotechnolgoy
Supercritical fluids
Tissue engineering
marine biotechnology
Science & Technology
topic Collagen
Hydrogels
Marine biotechnolgoy
Supercritical fluids
Tissue engineering
marine biotechnology
Science & Technology
description The possibility to fabricate marine collagen porous structures crosslinked with genipin under high pressure carbon dioxide is investigated. Collagen from shark skin is used to prepare prescaffolds by freeze-drying. The poor stability of the structures and low mechanical properties require crosslinking of the structures. Under dense CO2 atmosphere, crosslinking of collagen pre-scaffolds is allowed for 16 h. Additionally, the hydrogels are foamed and the scaffolds obtained present a highly porous structure. In vitro cell culture tests performed with a chondrocyte-like cell line show good cell adherence and proliferation, which is a strong indication of the potential of these scaffolds to be used in tissue cartilage tissue engineering.
publishDate 2013
dc.date.none.fl_str_mv 2013-09
2013-09-01T00:00:00Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://hdl.handle.net/1822/25167
url http://hdl.handle.net/1822/25167
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv 1616-5195
10.1002/mabi.201300228
24039034
http://onlinelibrary.wiley.com/doi/10.1002/mabi.201300228/abstract
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Wiley
publisher.none.fl_str_mv Wiley
dc.source.none.fl_str_mv reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron:RCAAP
instname_str Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
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collection Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
repository.name.fl_str_mv Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
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