Genistein and estradiol have common and specific impacts on the sea bass (Dicentrarchus labrax) skin-scale barrier

Detalhes bibliográficos
Autor(a) principal: Pinto, Patricia IS
Data de Publicação: 2019
Outros Autores: Andrade, André, Moreira, Catarina, Zapater, Cinta, Thorne, Michael A.S., Santos, Soraia, Estêvão, M. Dulce, Gomez, Ana, Canario, Adelino, Power, Deborah
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
Texto Completo: http://hdl.handle.net/10400.1/13636
Resumo: Teleost fish scales play important roles in animal protection and homeostasis. They can be targeted by endogenous estrogens and by environmental estrogenic endocrine disruptors. The phytoestrogen genistein is ubiquitous in the environment and in aquaculture feeds and is a disruptor of estrogenic processes in vertebrates. To test genistein disrupting actions in teleost fish we used a minimally invasive approach by analysing scales plucked from the skin of sea bass (Dicentrarchus labrax). Genistein transactivated all three fish nuclear estrogen receptors and was most potent with the Esr2, had the highest efficacy with Esr1, but reached, in all cases, transactivation levels lower than those of estradiol. RNA-seq revealed 254 responsive genes in the sea bass scales transcriptome with an FDR < 0.05 and more than 2-fold change in expression, 1 or 5 days after acute exposure to estradiol or to genistein. 65 genes were specifically responsive to estradiol and 106 by genistein while 83 genes were responsive to both compounds. Estradiol specifically regulated genes of protein/matrix turnover and genistein affected sterol biosynthesis and regeneration, while innate immune responses were affected by both compounds. This comprehensive study revealed the impact on the fish scale transcriptome of estradiol and genistein, providing a solid background to further develop fish scales as a practical screening tool for endocrine disrupting chemicals in teleosts.
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spelling Genistein and estradiol have common and specific impacts on the sea bass (Dicentrarchus labrax) skin-scale barrierAnimal scalesAnimalsBassEndocrine disruptorsEstradiolGenisteinHEK293 cellsHumansReceptorsSkinTranscriptomeTeleost fish scales play important roles in animal protection and homeostasis. They can be targeted by endogenous estrogens and by environmental estrogenic endocrine disruptors. The phytoestrogen genistein is ubiquitous in the environment and in aquaculture feeds and is a disruptor of estrogenic processes in vertebrates. To test genistein disrupting actions in teleost fish we used a minimally invasive approach by analysing scales plucked from the skin of sea bass (Dicentrarchus labrax). Genistein transactivated all three fish nuclear estrogen receptors and was most potent with the Esr2, had the highest efficacy with Esr1, but reached, in all cases, transactivation levels lower than those of estradiol. RNA-seq revealed 254 responsive genes in the sea bass scales transcriptome with an FDR < 0.05 and more than 2-fold change in expression, 1 or 5 days after acute exposure to estradiol or to genistein. 65 genes were specifically responsive to estradiol and 106 by genistein while 83 genes were responsive to both compounds. Estradiol specifically regulated genes of protein/matrix turnover and genistein affected sterol biosynthesis and regeneration, while innate immune responses were affected by both compounds. This comprehensive study revealed the impact on the fish scale transcriptome of estradiol and genistein, providing a solid background to further develop fish scales as a practical screening tool for endocrine disrupting chemicals in teleosts.AGL2015-67477-C2-1-RPergamon-ElsevierSapientiaPinto, Patricia ISAndrade, AndréMoreira, CatarinaZapater, CintaThorne, Michael A.S.Santos, SoraiaEstêvão, M. DulceGomez, AnaCanario, AdelinoPower, Deborah2020-12-01T01:30:14Z20192019-01-01T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/10400.1/13636eng0960-076010.1016/j.jsbmb.2019.105448info:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2024-03-06T02:02:19Zoai:sapientia.ualg.pt:10400.1/13636Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-19T20:04:46.898669Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse
dc.title.none.fl_str_mv Genistein and estradiol have common and specific impacts on the sea bass (Dicentrarchus labrax) skin-scale barrier
title Genistein and estradiol have common and specific impacts on the sea bass (Dicentrarchus labrax) skin-scale barrier
spellingShingle Genistein and estradiol have common and specific impacts on the sea bass (Dicentrarchus labrax) skin-scale barrier
Pinto, Patricia IS
Animal scales
Animals
Bass
Endocrine disruptors
Estradiol
Genistein
HEK293 cells
Humans
Receptors
Skin
Transcriptome
title_short Genistein and estradiol have common and specific impacts on the sea bass (Dicentrarchus labrax) skin-scale barrier
title_full Genistein and estradiol have common and specific impacts on the sea bass (Dicentrarchus labrax) skin-scale barrier
title_fullStr Genistein and estradiol have common and specific impacts on the sea bass (Dicentrarchus labrax) skin-scale barrier
title_full_unstemmed Genistein and estradiol have common and specific impacts on the sea bass (Dicentrarchus labrax) skin-scale barrier
title_sort Genistein and estradiol have common and specific impacts on the sea bass (Dicentrarchus labrax) skin-scale barrier
author Pinto, Patricia IS
author_facet Pinto, Patricia IS
Andrade, André
Moreira, Catarina
Zapater, Cinta
Thorne, Michael A.S.
Santos, Soraia
Estêvão, M. Dulce
Gomez, Ana
Canario, Adelino
Power, Deborah
author_role author
author2 Andrade, André
Moreira, Catarina
Zapater, Cinta
Thorne, Michael A.S.
Santos, Soraia
Estêvão, M. Dulce
Gomez, Ana
Canario, Adelino
Power, Deborah
author2_role author
author
author
author
author
author
author
author
author
dc.contributor.none.fl_str_mv Sapientia
dc.contributor.author.fl_str_mv Pinto, Patricia IS
Andrade, André
Moreira, Catarina
Zapater, Cinta
Thorne, Michael A.S.
Santos, Soraia
Estêvão, M. Dulce
Gomez, Ana
Canario, Adelino
Power, Deborah
dc.subject.por.fl_str_mv Animal scales
Animals
Bass
Endocrine disruptors
Estradiol
Genistein
HEK293 cells
Humans
Receptors
Skin
Transcriptome
topic Animal scales
Animals
Bass
Endocrine disruptors
Estradiol
Genistein
HEK293 cells
Humans
Receptors
Skin
Transcriptome
description Teleost fish scales play important roles in animal protection and homeostasis. They can be targeted by endogenous estrogens and by environmental estrogenic endocrine disruptors. The phytoestrogen genistein is ubiquitous in the environment and in aquaculture feeds and is a disruptor of estrogenic processes in vertebrates. To test genistein disrupting actions in teleost fish we used a minimally invasive approach by analysing scales plucked from the skin of sea bass (Dicentrarchus labrax). Genistein transactivated all three fish nuclear estrogen receptors and was most potent with the Esr2, had the highest efficacy with Esr1, but reached, in all cases, transactivation levels lower than those of estradiol. RNA-seq revealed 254 responsive genes in the sea bass scales transcriptome with an FDR < 0.05 and more than 2-fold change in expression, 1 or 5 days after acute exposure to estradiol or to genistein. 65 genes were specifically responsive to estradiol and 106 by genistein while 83 genes were responsive to both compounds. Estradiol specifically regulated genes of protein/matrix turnover and genistein affected sterol biosynthesis and regeneration, while innate immune responses were affected by both compounds. This comprehensive study revealed the impact on the fish scale transcriptome of estradiol and genistein, providing a solid background to further develop fish scales as a practical screening tool for endocrine disrupting chemicals in teleosts.
publishDate 2019
dc.date.none.fl_str_mv 2019
2019-01-01T00:00:00Z
2020-12-01T01:30:14Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://hdl.handle.net/10400.1/13636
url http://hdl.handle.net/10400.1/13636
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv 0960-0760
10.1016/j.jsbmb.2019.105448
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Pergamon-Elsevier
publisher.none.fl_str_mv Pergamon-Elsevier
dc.source.none.fl_str_mv reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron:RCAAP
instname_str Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron_str RCAAP
institution RCAAP
reponame_str Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
collection Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
repository.name.fl_str_mv Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
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