Genistein and estradiol have common and specific impacts on the sea bass (Dicentrarchus labrax) skin-scale barrier
Autor(a) principal: | |
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Data de Publicação: | 2019 |
Outros Autores: | , , , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
Texto Completo: | http://hdl.handle.net/10400.1/13636 |
Resumo: | Teleost fish scales play important roles in animal protection and homeostasis. They can be targeted by endogenous estrogens and by environmental estrogenic endocrine disruptors. The phytoestrogen genistein is ubiquitous in the environment and in aquaculture feeds and is a disruptor of estrogenic processes in vertebrates. To test genistein disrupting actions in teleost fish we used a minimally invasive approach by analysing scales plucked from the skin of sea bass (Dicentrarchus labrax). Genistein transactivated all three fish nuclear estrogen receptors and was most potent with the Esr2, had the highest efficacy with Esr1, but reached, in all cases, transactivation levels lower than those of estradiol. RNA-seq revealed 254 responsive genes in the sea bass scales transcriptome with an FDR < 0.05 and more than 2-fold change in expression, 1 or 5 days after acute exposure to estradiol or to genistein. 65 genes were specifically responsive to estradiol and 106 by genistein while 83 genes were responsive to both compounds. Estradiol specifically regulated genes of protein/matrix turnover and genistein affected sterol biosynthesis and regeneration, while innate immune responses were affected by both compounds. This comprehensive study revealed the impact on the fish scale transcriptome of estradiol and genistein, providing a solid background to further develop fish scales as a practical screening tool for endocrine disrupting chemicals in teleosts. |
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Genistein and estradiol have common and specific impacts on the sea bass (Dicentrarchus labrax) skin-scale barrierAnimal scalesAnimalsBassEndocrine disruptorsEstradiolGenisteinHEK293 cellsHumansReceptorsSkinTranscriptomeTeleost fish scales play important roles in animal protection and homeostasis. They can be targeted by endogenous estrogens and by environmental estrogenic endocrine disruptors. The phytoestrogen genistein is ubiquitous in the environment and in aquaculture feeds and is a disruptor of estrogenic processes in vertebrates. To test genistein disrupting actions in teleost fish we used a minimally invasive approach by analysing scales plucked from the skin of sea bass (Dicentrarchus labrax). Genistein transactivated all three fish nuclear estrogen receptors and was most potent with the Esr2, had the highest efficacy with Esr1, but reached, in all cases, transactivation levels lower than those of estradiol. RNA-seq revealed 254 responsive genes in the sea bass scales transcriptome with an FDR < 0.05 and more than 2-fold change in expression, 1 or 5 days after acute exposure to estradiol or to genistein. 65 genes were specifically responsive to estradiol and 106 by genistein while 83 genes were responsive to both compounds. Estradiol specifically regulated genes of protein/matrix turnover and genistein affected sterol biosynthesis and regeneration, while innate immune responses were affected by both compounds. This comprehensive study revealed the impact on the fish scale transcriptome of estradiol and genistein, providing a solid background to further develop fish scales as a practical screening tool for endocrine disrupting chemicals in teleosts.AGL2015-67477-C2-1-RPergamon-ElsevierSapientiaPinto, Patricia ISAndrade, AndréMoreira, CatarinaZapater, CintaThorne, Michael A.S.Santos, SoraiaEstêvão, M. DulceGomez, AnaCanario, AdelinoPower, Deborah2020-12-01T01:30:14Z20192019-01-01T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/10400.1/13636eng0960-076010.1016/j.jsbmb.2019.105448info:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2024-03-06T02:02:19Zoai:sapientia.ualg.pt:10400.1/13636Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-19T20:04:46.898669Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse |
dc.title.none.fl_str_mv |
Genistein and estradiol have common and specific impacts on the sea bass (Dicentrarchus labrax) skin-scale barrier |
title |
Genistein and estradiol have common and specific impacts on the sea bass (Dicentrarchus labrax) skin-scale barrier |
spellingShingle |
Genistein and estradiol have common and specific impacts on the sea bass (Dicentrarchus labrax) skin-scale barrier Pinto, Patricia IS Animal scales Animals Bass Endocrine disruptors Estradiol Genistein HEK293 cells Humans Receptors Skin Transcriptome |
title_short |
Genistein and estradiol have common and specific impacts on the sea bass (Dicentrarchus labrax) skin-scale barrier |
title_full |
Genistein and estradiol have common and specific impacts on the sea bass (Dicentrarchus labrax) skin-scale barrier |
title_fullStr |
Genistein and estradiol have common and specific impacts on the sea bass (Dicentrarchus labrax) skin-scale barrier |
title_full_unstemmed |
Genistein and estradiol have common and specific impacts on the sea bass (Dicentrarchus labrax) skin-scale barrier |
title_sort |
Genistein and estradiol have common and specific impacts on the sea bass (Dicentrarchus labrax) skin-scale barrier |
author |
Pinto, Patricia IS |
author_facet |
Pinto, Patricia IS Andrade, André Moreira, Catarina Zapater, Cinta Thorne, Michael A.S. Santos, Soraia Estêvão, M. Dulce Gomez, Ana Canario, Adelino Power, Deborah |
author_role |
author |
author2 |
Andrade, André Moreira, Catarina Zapater, Cinta Thorne, Michael A.S. Santos, Soraia Estêvão, M. Dulce Gomez, Ana Canario, Adelino Power, Deborah |
author2_role |
author author author author author author author author author |
dc.contributor.none.fl_str_mv |
Sapientia |
dc.contributor.author.fl_str_mv |
Pinto, Patricia IS Andrade, André Moreira, Catarina Zapater, Cinta Thorne, Michael A.S. Santos, Soraia Estêvão, M. Dulce Gomez, Ana Canario, Adelino Power, Deborah |
dc.subject.por.fl_str_mv |
Animal scales Animals Bass Endocrine disruptors Estradiol Genistein HEK293 cells Humans Receptors Skin Transcriptome |
topic |
Animal scales Animals Bass Endocrine disruptors Estradiol Genistein HEK293 cells Humans Receptors Skin Transcriptome |
description |
Teleost fish scales play important roles in animal protection and homeostasis. They can be targeted by endogenous estrogens and by environmental estrogenic endocrine disruptors. The phytoestrogen genistein is ubiquitous in the environment and in aquaculture feeds and is a disruptor of estrogenic processes in vertebrates. To test genistein disrupting actions in teleost fish we used a minimally invasive approach by analysing scales plucked from the skin of sea bass (Dicentrarchus labrax). Genistein transactivated all three fish nuclear estrogen receptors and was most potent with the Esr2, had the highest efficacy with Esr1, but reached, in all cases, transactivation levels lower than those of estradiol. RNA-seq revealed 254 responsive genes in the sea bass scales transcriptome with an FDR < 0.05 and more than 2-fold change in expression, 1 or 5 days after acute exposure to estradiol or to genistein. 65 genes were specifically responsive to estradiol and 106 by genistein while 83 genes were responsive to both compounds. Estradiol specifically regulated genes of protein/matrix turnover and genistein affected sterol biosynthesis and regeneration, while innate immune responses were affected by both compounds. This comprehensive study revealed the impact on the fish scale transcriptome of estradiol and genistein, providing a solid background to further develop fish scales as a practical screening tool for endocrine disrupting chemicals in teleosts. |
publishDate |
2019 |
dc.date.none.fl_str_mv |
2019 2019-01-01T00:00:00Z 2020-12-01T01:30:14Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://hdl.handle.net/10400.1/13636 |
url |
http://hdl.handle.net/10400.1/13636 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
0960-0760 10.1016/j.jsbmb.2019.105448 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.publisher.none.fl_str_mv |
Pergamon-Elsevier |
publisher.none.fl_str_mv |
Pergamon-Elsevier |
dc.source.none.fl_str_mv |
reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação instacron:RCAAP |
instname_str |
Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
instacron_str |
RCAAP |
institution |
RCAAP |
reponame_str |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
collection |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
repository.name.fl_str_mv |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
repository.mail.fl_str_mv |
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1799133285353259008 |