Estrogen and genistein modulation of bone homeostasis in the teleost, sea bass Dicentrarchus labrax: a proteomic approach

Detalhes bibliográficos
Autor(a) principal: Santos, Rui Miguel Rodrigues dos
Data de Publicação: 2015
Tipo de documento: Dissertação
Idioma: eng
Título da fonte: Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
Texto Completo: http://hdl.handle.net/10400.1/7685
Resumo: Dissertação de mestrado em Biotecnologia, Faculdade de Ciências e Tecnologia, Univ. do Algarve
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spelling Estrogen and genistein modulation of bone homeostasis in the teleost, sea bass Dicentrarchus labrax: a proteomic approachSea bassBone proteomeBone turnoverGenistein17-B-estradiolDomínio/Área Científica::Engenharia e Tecnologia::Biotecnologia AmbientalDissertação de mestrado em Biotecnologia, Faculdade de Ciências e Tecnologia, Univ. do AlgarveThe skeleton in vertebrates is in constant turnover and functions as a reservoir of minerals. The regulation of bone turnover in vertebrates is a complex process and diet and hormones have a central role. Estrogen, a steroid, which signals via multiple nuclear and membrane receptors, is important in the turnover of bone in vertebrates. In humans lack of estrogen causes osteoporosis (bone thinning). The way in which estrogen regulates bone turnover is still relatively poorly described. The present thesis reports an experiment performed to assess how estrogen and the phytoestrogen, genistein, affect the bone proteome of a teleost, the sea bass, Dicentrarchus labrax. For the experiment an intraperitoneal injection of 17-β-estradiol (E2) (5mg/Kg body mass), Genistein (5mg/Kg body mass) and coconut oil (the vehicle) was administered to 30 immature sea bass (10 for each treatment) for 5 days and samples of blood and bone were collected. Plasma parameters like calcium, estrogen and vitellogenin were significantly (p<0.05) increased by E2. Genistein only increased vitellogenin. The two treatment did not modify bone metabolism and tartrate-resistant acid phosphatase (TRAP) and alkaline phosphatase (ALP) did not change significantly between treatment groups. Vertebral bone proteome was established and a total of 285 protein spots were detected and used for comparison between experimental groups. Analysis of the gels showed that 8 and 22 protein spots were differentially expressed (p<0.05) in vertebra from E2 and genistein treatment respectively. Of the 8 protein modified by E2, only 4 were identified. In the genistein treatment, of the 22 proteins differentially expressed only 10 were identified and 2 were the same as found in the E2 group Tropomyosin alpha-4 chain (TPM4) and Myosing binding protein C cardiac type like (MYPCL3). Identification and biological process were described using Uniprot, NCBI and gene ontology. Proteins differentially expressed in both treatments that were down-regulated were related to calcium ion binding, muscle contraction, cell adhesion, transport, protein targeting and homeostasis. In conclusion E2 and genistein did not modify indicators of bone turnover but modifications in the bone proteome occurred. A final step still required is the validation of the proteome results by Western blotting of selected proteins.Power, DeborahValls, Antonio IbarzSapientiaSantos, Rui Miguel Rodrigues dos2020-09-10T00:30:08Z201520152015-01-01T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/masterThesisapplication/pdfhttp://hdl.handle.net/10400.1/7685TID:202227405enginfo:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2023-07-24T10:18:49Zoai:sapientia.ualg.pt:10400.1/7685Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-19T19:59:59.825455Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse
dc.title.none.fl_str_mv Estrogen and genistein modulation of bone homeostasis in the teleost, sea bass Dicentrarchus labrax: a proteomic approach
title Estrogen and genistein modulation of bone homeostasis in the teleost, sea bass Dicentrarchus labrax: a proteomic approach
spellingShingle Estrogen and genistein modulation of bone homeostasis in the teleost, sea bass Dicentrarchus labrax: a proteomic approach
Santos, Rui Miguel Rodrigues dos
Sea bass
Bone proteome
Bone turnover
Genistein
17-B-estradiol
Domínio/Área Científica::Engenharia e Tecnologia::Biotecnologia Ambiental
title_short Estrogen and genistein modulation of bone homeostasis in the teleost, sea bass Dicentrarchus labrax: a proteomic approach
title_full Estrogen and genistein modulation of bone homeostasis in the teleost, sea bass Dicentrarchus labrax: a proteomic approach
title_fullStr Estrogen and genistein modulation of bone homeostasis in the teleost, sea bass Dicentrarchus labrax: a proteomic approach
title_full_unstemmed Estrogen and genistein modulation of bone homeostasis in the teleost, sea bass Dicentrarchus labrax: a proteomic approach
title_sort Estrogen and genistein modulation of bone homeostasis in the teleost, sea bass Dicentrarchus labrax: a proteomic approach
author Santos, Rui Miguel Rodrigues dos
author_facet Santos, Rui Miguel Rodrigues dos
author_role author
dc.contributor.none.fl_str_mv Power, Deborah
Valls, Antonio Ibarz
Sapientia
dc.contributor.author.fl_str_mv Santos, Rui Miguel Rodrigues dos
dc.subject.por.fl_str_mv Sea bass
Bone proteome
Bone turnover
Genistein
17-B-estradiol
Domínio/Área Científica::Engenharia e Tecnologia::Biotecnologia Ambiental
topic Sea bass
Bone proteome
Bone turnover
Genistein
17-B-estradiol
Domínio/Área Científica::Engenharia e Tecnologia::Biotecnologia Ambiental
description Dissertação de mestrado em Biotecnologia, Faculdade de Ciências e Tecnologia, Univ. do Algarve
publishDate 2015
dc.date.none.fl_str_mv 2015
2015
2015-01-01T00:00:00Z
2020-09-10T00:30:08Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/masterThesis
format masterThesis
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://hdl.handle.net/10400.1/7685
TID:202227405
url http://hdl.handle.net/10400.1/7685
identifier_str_mv TID:202227405
dc.language.iso.fl_str_mv eng
language eng
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.source.none.fl_str_mv reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron:RCAAP
instname_str Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron_str RCAAP
institution RCAAP
reponame_str Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
collection Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
repository.name.fl_str_mv Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
repository.mail.fl_str_mv
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