Prevalence of pfk13 and pfmdr1 polymorphisms in Bounkiling, Southern Senegal
Autor(a) principal: | |
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Data de Publicação: | 2021 |
Outros Autores: | , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
Texto Completo: | http://hdl.handle.net/10362/116520 |
Resumo: | BACKGROUND: Delayed Plasmodium falciparum parasite clearance has been associated with Single Nucleotide Polymorphisms (SNPs) in the kelch protein propeller domain (coded by pfk13 gene). SNPs in the Plasmodium falciparum multidrug resistance gene 1 (pfmdr1) are associated with multi-drug resistance including the combination artemether-lumefantrine. To our knowledge, this is the first work providing information on the prevalence of k13-propeller and pfmdr1 mutations from Sédhiou, a region in the south of Senegal. METHODS: 147 dried blood spots on filter papers were collected from symptomatic patients attending a hospital located in Bounkiling City, Sédhiou Region, Southern Senegal. All samples were collected between 2015-2017 during the malaria transmission season. Specific regions of the gene pfk13 and pfmdr1 were analyzed using PCR amplification and Sanger sequencing. RESULTS: The majority of parasites (92.9%) harboured the pfk13 wild type sequence and 6 samples harboured synonymous changes. Regarding pfmdr1, wild-type alleles represented the majority except at codon 184. Overall, prevalence of 86Y was 11.9%, 184F was 56.3% and 1246Y was 1.5%. The mutant allele 184F decreased from 73.7% in 2015 to 40.7% in 2017. The prevalence of haplotype NFD decreased from 71.4% in 2015 to 20.8% in 2017. CONCLUSIONS: This study provides the first description of pfk13 and pfmdr1 genes variations in Bounkiling, a city in the Sédhiou Region of Senegal, contributing to closing the gap of information on anti-malaria drug resistance molecular markers in southern Senegal. |
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Prevalence of pfk13 and pfmdr1 polymorphisms in Bounkiling, Southern SenegalGeneticsParasitologyInfectious DiseasesSDG 3 - Good Health and Well-beingSDG 10 - Reduced InequalitiesSDG 12 - Responsible Consumption and ProductionBACKGROUND: Delayed Plasmodium falciparum parasite clearance has been associated with Single Nucleotide Polymorphisms (SNPs) in the kelch protein propeller domain (coded by pfk13 gene). SNPs in the Plasmodium falciparum multidrug resistance gene 1 (pfmdr1) are associated with multi-drug resistance including the combination artemether-lumefantrine. To our knowledge, this is the first work providing information on the prevalence of k13-propeller and pfmdr1 mutations from Sédhiou, a region in the south of Senegal. METHODS: 147 dried blood spots on filter papers were collected from symptomatic patients attending a hospital located in Bounkiling City, Sédhiou Region, Southern Senegal. All samples were collected between 2015-2017 during the malaria transmission season. Specific regions of the gene pfk13 and pfmdr1 were analyzed using PCR amplification and Sanger sequencing. RESULTS: The majority of parasites (92.9%) harboured the pfk13 wild type sequence and 6 samples harboured synonymous changes. Regarding pfmdr1, wild-type alleles represented the majority except at codon 184. Overall, prevalence of 86Y was 11.9%, 184F was 56.3% and 1246Y was 1.5%. The mutant allele 184F decreased from 73.7% in 2015 to 40.7% in 2017. The prevalence of haplotype NFD decreased from 71.4% in 2015 to 20.8% in 2017. CONCLUSIONS: This study provides the first description of pfk13 and pfmdr1 genes variations in Bounkiling, a city in the Sédhiou Region of Senegal, contributing to closing the gap of information on anti-malaria drug resistance molecular markers in southern Senegal.Global Health and Tropical Medicine (GHTM)Instituto de Higiene e Medicina Tropical (IHMT)Vector borne diseases and pathogens (VBD)RUNAhouidi, AmbroiseOliveira, RafaelLobo, LisDiedhiou, CyrilleMboup, SouleymaneNogueira, Fatima2021-04-30T22:44:26Z2021-03-262021-03-26T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article11application/pdfhttp://hdl.handle.net/10362/116520eng1932-6203PURE: 28935520https://doi.org/10.1371/journal.pone.0249357info:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2024-03-11T04:59:09Zoai:run.unl.pt:10362/116520Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-20T03:43:06.988497Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse |
dc.title.none.fl_str_mv |
Prevalence of pfk13 and pfmdr1 polymorphisms in Bounkiling, Southern Senegal |
title |
Prevalence of pfk13 and pfmdr1 polymorphisms in Bounkiling, Southern Senegal |
spellingShingle |
Prevalence of pfk13 and pfmdr1 polymorphisms in Bounkiling, Southern Senegal Ahouidi, Ambroise Genetics Parasitology Infectious Diseases SDG 3 - Good Health and Well-being SDG 10 - Reduced Inequalities SDG 12 - Responsible Consumption and Production |
title_short |
Prevalence of pfk13 and pfmdr1 polymorphisms in Bounkiling, Southern Senegal |
title_full |
Prevalence of pfk13 and pfmdr1 polymorphisms in Bounkiling, Southern Senegal |
title_fullStr |
Prevalence of pfk13 and pfmdr1 polymorphisms in Bounkiling, Southern Senegal |
title_full_unstemmed |
Prevalence of pfk13 and pfmdr1 polymorphisms in Bounkiling, Southern Senegal |
title_sort |
Prevalence of pfk13 and pfmdr1 polymorphisms in Bounkiling, Southern Senegal |
author |
Ahouidi, Ambroise |
author_facet |
Ahouidi, Ambroise Oliveira, Rafael Lobo, Lis Diedhiou, Cyrille Mboup, Souleymane Nogueira, Fatima |
author_role |
author |
author2 |
Oliveira, Rafael Lobo, Lis Diedhiou, Cyrille Mboup, Souleymane Nogueira, Fatima |
author2_role |
author author author author author |
dc.contributor.none.fl_str_mv |
Global Health and Tropical Medicine (GHTM) Instituto de Higiene e Medicina Tropical (IHMT) Vector borne diseases and pathogens (VBD) RUN |
dc.contributor.author.fl_str_mv |
Ahouidi, Ambroise Oliveira, Rafael Lobo, Lis Diedhiou, Cyrille Mboup, Souleymane Nogueira, Fatima |
dc.subject.por.fl_str_mv |
Genetics Parasitology Infectious Diseases SDG 3 - Good Health and Well-being SDG 10 - Reduced Inequalities SDG 12 - Responsible Consumption and Production |
topic |
Genetics Parasitology Infectious Diseases SDG 3 - Good Health and Well-being SDG 10 - Reduced Inequalities SDG 12 - Responsible Consumption and Production |
description |
BACKGROUND: Delayed Plasmodium falciparum parasite clearance has been associated with Single Nucleotide Polymorphisms (SNPs) in the kelch protein propeller domain (coded by pfk13 gene). SNPs in the Plasmodium falciparum multidrug resistance gene 1 (pfmdr1) are associated with multi-drug resistance including the combination artemether-lumefantrine. To our knowledge, this is the first work providing information on the prevalence of k13-propeller and pfmdr1 mutations from Sédhiou, a region in the south of Senegal. METHODS: 147 dried blood spots on filter papers were collected from symptomatic patients attending a hospital located in Bounkiling City, Sédhiou Region, Southern Senegal. All samples were collected between 2015-2017 during the malaria transmission season. Specific regions of the gene pfk13 and pfmdr1 were analyzed using PCR amplification and Sanger sequencing. RESULTS: The majority of parasites (92.9%) harboured the pfk13 wild type sequence and 6 samples harboured synonymous changes. Regarding pfmdr1, wild-type alleles represented the majority except at codon 184. Overall, prevalence of 86Y was 11.9%, 184F was 56.3% and 1246Y was 1.5%. The mutant allele 184F decreased from 73.7% in 2015 to 40.7% in 2017. The prevalence of haplotype NFD decreased from 71.4% in 2015 to 20.8% in 2017. CONCLUSIONS: This study provides the first description of pfk13 and pfmdr1 genes variations in Bounkiling, a city in the Sédhiou Region of Senegal, contributing to closing the gap of information on anti-malaria drug resistance molecular markers in southern Senegal. |
publishDate |
2021 |
dc.date.none.fl_str_mv |
2021-04-30T22:44:26Z 2021-03-26 2021-03-26T00:00:00Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://hdl.handle.net/10362/116520 |
url |
http://hdl.handle.net/10362/116520 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
1932-6203 PURE: 28935520 https://doi.org/10.1371/journal.pone.0249357 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
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11 application/pdf |
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Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
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RCAAP |
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RCAAP |
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Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
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Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
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Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
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