Prevalence of pfk13 and pfmdr1 polymorphisms in Bounkiling, Southern Senegal

Detalhes bibliográficos
Autor(a) principal: Ahouidi, Ambroise
Data de Publicação: 2021
Outros Autores: Oliveira, Rafael, Lobo, Lis, Diedhiou, Cyrille, Mboup, Souleymane, Nogueira, Fatima
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
Texto Completo: http://hdl.handle.net/10362/116520
Resumo: BACKGROUND: Delayed Plasmodium falciparum parasite clearance has been associated with Single Nucleotide Polymorphisms (SNPs) in the kelch protein propeller domain (coded by pfk13 gene). SNPs in the Plasmodium falciparum multidrug resistance gene 1 (pfmdr1) are associated with multi-drug resistance including the combination artemether-lumefantrine. To our knowledge, this is the first work providing information on the prevalence of k13-propeller and pfmdr1 mutations from Sédhiou, a region in the south of Senegal. METHODS: 147 dried blood spots on filter papers were collected from symptomatic patients attending a hospital located in Bounkiling City, Sédhiou Region, Southern Senegal. All samples were collected between 2015-2017 during the malaria transmission season. Specific regions of the gene pfk13 and pfmdr1 were analyzed using PCR amplification and Sanger sequencing. RESULTS: The majority of parasites (92.9%) harboured the pfk13 wild type sequence and 6 samples harboured synonymous changes. Regarding pfmdr1, wild-type alleles represented the majority except at codon 184. Overall, prevalence of 86Y was 11.9%, 184F was 56.3% and 1246Y was 1.5%. The mutant allele 184F decreased from 73.7% in 2015 to 40.7% in 2017. The prevalence of haplotype NFD decreased from 71.4% in 2015 to 20.8% in 2017. CONCLUSIONS: This study provides the first description of pfk13 and pfmdr1 genes variations in Bounkiling, a city in the Sédhiou Region of Senegal, contributing to closing the gap of information on anti-malaria drug resistance molecular markers in southern Senegal.
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spelling Prevalence of pfk13 and pfmdr1 polymorphisms in Bounkiling, Southern SenegalGeneticsParasitologyInfectious DiseasesSDG 3 - Good Health and Well-beingSDG 10 - Reduced InequalitiesSDG 12 - Responsible Consumption and ProductionBACKGROUND: Delayed Plasmodium falciparum parasite clearance has been associated with Single Nucleotide Polymorphisms (SNPs) in the kelch protein propeller domain (coded by pfk13 gene). SNPs in the Plasmodium falciparum multidrug resistance gene 1 (pfmdr1) are associated with multi-drug resistance including the combination artemether-lumefantrine. To our knowledge, this is the first work providing information on the prevalence of k13-propeller and pfmdr1 mutations from Sédhiou, a region in the south of Senegal. METHODS: 147 dried blood spots on filter papers were collected from symptomatic patients attending a hospital located in Bounkiling City, Sédhiou Region, Southern Senegal. All samples were collected between 2015-2017 during the malaria transmission season. Specific regions of the gene pfk13 and pfmdr1 were analyzed using PCR amplification and Sanger sequencing. RESULTS: The majority of parasites (92.9%) harboured the pfk13 wild type sequence and 6 samples harboured synonymous changes. Regarding pfmdr1, wild-type alleles represented the majority except at codon 184. Overall, prevalence of 86Y was 11.9%, 184F was 56.3% and 1246Y was 1.5%. The mutant allele 184F decreased from 73.7% in 2015 to 40.7% in 2017. The prevalence of haplotype NFD decreased from 71.4% in 2015 to 20.8% in 2017. CONCLUSIONS: This study provides the first description of pfk13 and pfmdr1 genes variations in Bounkiling, a city in the Sédhiou Region of Senegal, contributing to closing the gap of information on anti-malaria drug resistance molecular markers in southern Senegal.Global Health and Tropical Medicine (GHTM)Instituto de Higiene e Medicina Tropical (IHMT)Vector borne diseases and pathogens (VBD)RUNAhouidi, AmbroiseOliveira, RafaelLobo, LisDiedhiou, CyrilleMboup, SouleymaneNogueira, Fatima2021-04-30T22:44:26Z2021-03-262021-03-26T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article11application/pdfhttp://hdl.handle.net/10362/116520eng1932-6203PURE: 28935520https://doi.org/10.1371/journal.pone.0249357info:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2024-03-11T04:59:09Zoai:run.unl.pt:10362/116520Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-20T03:43:06.988497Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse
dc.title.none.fl_str_mv Prevalence of pfk13 and pfmdr1 polymorphisms in Bounkiling, Southern Senegal
title Prevalence of pfk13 and pfmdr1 polymorphisms in Bounkiling, Southern Senegal
spellingShingle Prevalence of pfk13 and pfmdr1 polymorphisms in Bounkiling, Southern Senegal
Ahouidi, Ambroise
Genetics
Parasitology
Infectious Diseases
SDG 3 - Good Health and Well-being
SDG 10 - Reduced Inequalities
SDG 12 - Responsible Consumption and Production
title_short Prevalence of pfk13 and pfmdr1 polymorphisms in Bounkiling, Southern Senegal
title_full Prevalence of pfk13 and pfmdr1 polymorphisms in Bounkiling, Southern Senegal
title_fullStr Prevalence of pfk13 and pfmdr1 polymorphisms in Bounkiling, Southern Senegal
title_full_unstemmed Prevalence of pfk13 and pfmdr1 polymorphisms in Bounkiling, Southern Senegal
title_sort Prevalence of pfk13 and pfmdr1 polymorphisms in Bounkiling, Southern Senegal
author Ahouidi, Ambroise
author_facet Ahouidi, Ambroise
Oliveira, Rafael
Lobo, Lis
Diedhiou, Cyrille
Mboup, Souleymane
Nogueira, Fatima
author_role author
author2 Oliveira, Rafael
Lobo, Lis
Diedhiou, Cyrille
Mboup, Souleymane
Nogueira, Fatima
author2_role author
author
author
author
author
dc.contributor.none.fl_str_mv Global Health and Tropical Medicine (GHTM)
Instituto de Higiene e Medicina Tropical (IHMT)
Vector borne diseases and pathogens (VBD)
RUN
dc.contributor.author.fl_str_mv Ahouidi, Ambroise
Oliveira, Rafael
Lobo, Lis
Diedhiou, Cyrille
Mboup, Souleymane
Nogueira, Fatima
dc.subject.por.fl_str_mv Genetics
Parasitology
Infectious Diseases
SDG 3 - Good Health and Well-being
SDG 10 - Reduced Inequalities
SDG 12 - Responsible Consumption and Production
topic Genetics
Parasitology
Infectious Diseases
SDG 3 - Good Health and Well-being
SDG 10 - Reduced Inequalities
SDG 12 - Responsible Consumption and Production
description BACKGROUND: Delayed Plasmodium falciparum parasite clearance has been associated with Single Nucleotide Polymorphisms (SNPs) in the kelch protein propeller domain (coded by pfk13 gene). SNPs in the Plasmodium falciparum multidrug resistance gene 1 (pfmdr1) are associated with multi-drug resistance including the combination artemether-lumefantrine. To our knowledge, this is the first work providing information on the prevalence of k13-propeller and pfmdr1 mutations from Sédhiou, a region in the south of Senegal. METHODS: 147 dried blood spots on filter papers were collected from symptomatic patients attending a hospital located in Bounkiling City, Sédhiou Region, Southern Senegal. All samples were collected between 2015-2017 during the malaria transmission season. Specific regions of the gene pfk13 and pfmdr1 were analyzed using PCR amplification and Sanger sequencing. RESULTS: The majority of parasites (92.9%) harboured the pfk13 wild type sequence and 6 samples harboured synonymous changes. Regarding pfmdr1, wild-type alleles represented the majority except at codon 184. Overall, prevalence of 86Y was 11.9%, 184F was 56.3% and 1246Y was 1.5%. The mutant allele 184F decreased from 73.7% in 2015 to 40.7% in 2017. The prevalence of haplotype NFD decreased from 71.4% in 2015 to 20.8% in 2017. CONCLUSIONS: This study provides the first description of pfk13 and pfmdr1 genes variations in Bounkiling, a city in the Sédhiou Region of Senegal, contributing to closing the gap of information on anti-malaria drug resistance molecular markers in southern Senegal.
publishDate 2021
dc.date.none.fl_str_mv 2021-04-30T22:44:26Z
2021-03-26
2021-03-26T00:00:00Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
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dc.identifier.uri.fl_str_mv http://hdl.handle.net/10362/116520
url http://hdl.handle.net/10362/116520
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv 1932-6203
PURE: 28935520
https://doi.org/10.1371/journal.pone.0249357
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
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application/pdf
dc.source.none.fl_str_mv reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
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reponame_str Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
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