The key role of sulfation and branching on fucoidan antitumor activity

Detalhes bibliográficos
Autor(a) principal: Oliveira, Catarina
Data de Publicação: 2017
Outros Autores: Ferreira, Andreia S., Novoa-Carballal, R., Nunes, Cláudia, Pashkuleva, I., Neves, N. M., Coimbra, Manuel A., Reis, R. L., Martins, Albino, Silva, Tiago José Quinteiros Lopes Henriques
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
Texto Completo: http://hdl.handle.net/1822/44382
Resumo: There is an urgent need for antitumor bioactive agents with minimal or no side effects over normal adjacent cells. Fucoidan is a marine-origin polymer with known antitumor activity. However, there are still some concerns about its application due to the inconsistent experimental results, specifically its toxicity over normal cells and the mechanism behind its action. Herein, three fucoidan extracts (FEs) have been tested over normal and breast cancer cell lines. From cytotoxicity results, only one of the extracts shows selective antitumor behavior (at 0.2 mg mLâ 1), despite similarities in sulfation degree and carbohydrates composition. Although the three FEs present different molecular weights, depolymerization of selected samples discarded Mw as the key factor in the antitumor activity. Significant differences in sulfates position and branching are observed, presenting FE 2 the higher branching degree. Based on all these experimental data, it is believed that these last two properties are the ones that influence the cytotoxic effects of fucoidan extracts.
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spelling The key role of sulfation and branching on fucoidan antitumor activityAntitumor activityFucoidanPolymer branchingStructure–activity relationshipSulfationScience & TechnologyThere is an urgent need for antitumor bioactive agents with minimal or no side effects over normal adjacent cells. Fucoidan is a marine-origin polymer with known antitumor activity. However, there are still some concerns about its application due to the inconsistent experimental results, specifically its toxicity over normal cells and the mechanism behind its action. Herein, three fucoidan extracts (FEs) have been tested over normal and breast cancer cell lines. From cytotoxicity results, only one of the extracts shows selective antitumor behavior (at 0.2 mg mLâ 1), despite similarities in sulfation degree and carbohydrates composition. Although the three FEs present different molecular weights, depolymerization of selected samples discarded Mw as the key factor in the antitumor activity. Significant differences in sulfates position and branching are observed, presenting FE 2 the higher branching degree. Based on all these experimental data, it is believed that these last two properties are the ones that influence the cytotoxic effects of fucoidan extracts.The authors would like to thank the funding from projects 0687_NOVOMAR_1_P, cofunded by INTERREG 2007-2013/POCTEP, CarbPol_u_Algae (EXPL/MAR-BIO/0165/2013), and IF/00376/2014/CP1212/CT0015, funded by the Portuguese Foundation for Science and Technology, FCT, and ComplexiTE (ERC-2012-ADG 20120216-321266), funded by the European Research Council under the European Union's Seventh Framework Programme for Research and Development. The authors would also like to thank FCT, Portugal, for the scholarship of A.S.F. (SFRH/BD/102471/2014), fellowship of C.N. (SFRH/BPD/100627/2014), Investigator grants of A.M. (IF/00376/2014), R.N.-C. (IF/00373/2014), and I.P. (IF/00032/2013) and the financial support to CICECO-Aveiro Institute of Materials (POCI-01-0145-FEDER-007679, FCT UID/CTM/50011/2013) and OOPNA (UID/OUI/00062/2013), through national founds and cofinanced by the FEDER, within the PT2020 Partnership Agreement.John Wiley and SonsUniversidade do MinhoOliveira, CatarinaFerreira, Andreia S.Novoa-Carballal, R.Nunes, CláudiaPashkuleva, I.Neves, N. M.Coimbra, Manuel A.Reis, R. L.Martins, AlbinoSilva, Tiago José Quinteiros Lopes Henriques20172017-01-01T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/1822/44382engOliveira C., Ferreira A. S., Novoa-Carballal R., Nunes C., Pashkuleva I., Neves N. M., Coimbra M. A., Reis R. L., Martins A., Silva T. H. The Key Role of Sulfation and Branching on Fucoidan Antitumor Activity, Macromolecular Bioscience, doi:10.1002/mabi.201600340, 20161616-519510.1002/mabi.20160034027996198http://dx.doi.org/10.1002/mabi.201600340info:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2023-07-21T12:17:44Zoai:repositorium.sdum.uminho.pt:1822/44382Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-19T19:10:24.704752Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse
dc.title.none.fl_str_mv The key role of sulfation and branching on fucoidan antitumor activity
title The key role of sulfation and branching on fucoidan antitumor activity
spellingShingle The key role of sulfation and branching on fucoidan antitumor activity
Oliveira, Catarina
Antitumor activity
Fucoidan
Polymer branching
Structure–activity relationship
Sulfation
Science & Technology
title_short The key role of sulfation and branching on fucoidan antitumor activity
title_full The key role of sulfation and branching on fucoidan antitumor activity
title_fullStr The key role of sulfation and branching on fucoidan antitumor activity
title_full_unstemmed The key role of sulfation and branching on fucoidan antitumor activity
title_sort The key role of sulfation and branching on fucoidan antitumor activity
author Oliveira, Catarina
author_facet Oliveira, Catarina
Ferreira, Andreia S.
Novoa-Carballal, R.
Nunes, Cláudia
Pashkuleva, I.
Neves, N. M.
Coimbra, Manuel A.
Reis, R. L.
Martins, Albino
Silva, Tiago José Quinteiros Lopes Henriques
author_role author
author2 Ferreira, Andreia S.
Novoa-Carballal, R.
Nunes, Cláudia
Pashkuleva, I.
Neves, N. M.
Coimbra, Manuel A.
Reis, R. L.
Martins, Albino
Silva, Tiago José Quinteiros Lopes Henriques
author2_role author
author
author
author
author
author
author
author
author
dc.contributor.none.fl_str_mv Universidade do Minho
dc.contributor.author.fl_str_mv Oliveira, Catarina
Ferreira, Andreia S.
Novoa-Carballal, R.
Nunes, Cláudia
Pashkuleva, I.
Neves, N. M.
Coimbra, Manuel A.
Reis, R. L.
Martins, Albino
Silva, Tiago José Quinteiros Lopes Henriques
dc.subject.por.fl_str_mv Antitumor activity
Fucoidan
Polymer branching
Structure–activity relationship
Sulfation
Science & Technology
topic Antitumor activity
Fucoidan
Polymer branching
Structure–activity relationship
Sulfation
Science & Technology
description There is an urgent need for antitumor bioactive agents with minimal or no side effects over normal adjacent cells. Fucoidan is a marine-origin polymer with known antitumor activity. However, there are still some concerns about its application due to the inconsistent experimental results, specifically its toxicity over normal cells and the mechanism behind its action. Herein, three fucoidan extracts (FEs) have been tested over normal and breast cancer cell lines. From cytotoxicity results, only one of the extracts shows selective antitumor behavior (at 0.2 mg mLâ 1), despite similarities in sulfation degree and carbohydrates composition. Although the three FEs present different molecular weights, depolymerization of selected samples discarded Mw as the key factor in the antitumor activity. Significant differences in sulfates position and branching are observed, presenting FE 2 the higher branching degree. Based on all these experimental data, it is believed that these last two properties are the ones that influence the cytotoxic effects of fucoidan extracts.
publishDate 2017
dc.date.none.fl_str_mv 2017
2017-01-01T00:00:00Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://hdl.handle.net/1822/44382
url http://hdl.handle.net/1822/44382
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv Oliveira C., Ferreira A. S., Novoa-Carballal R., Nunes C., Pashkuleva I., Neves N. M., Coimbra M. A., Reis R. L., Martins A., Silva T. H. The Key Role of Sulfation and Branching on Fucoidan Antitumor Activity, Macromolecular Bioscience, doi:10.1002/mabi.201600340, 2016
1616-5195
10.1002/mabi.201600340
27996198
http://dx.doi.org/10.1002/mabi.201600340
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv John Wiley and Sons
publisher.none.fl_str_mv John Wiley and Sons
dc.source.none.fl_str_mv reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron:RCAAP
instname_str Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
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reponame_str Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
collection Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
repository.name.fl_str_mv Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
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